 Beta-strand mimetics and method relating thereto
专利摘要:
公开号:AU2003249672A1 申请号:U2003249672 申请日:2003-05-30 公开日:2005-01-04 发明作者:Jae Uk Chung；Michael Kahn；Eguchi Masakatsu；Sung Hwan Moon 申请人:Choongwae Pharmaceutical Co Ltd； IPC主号:A61K31-519
专利说明:
WO 2004/108731 PCT/US2003/017188 BETA-STRAND MIMETICS AND METHODS RELATING THERETO BACKGROUND OF THE INVENTION Field of the Invention The present invention relates generally to P-strand mimetic 5 structures, to a chemical library relating thereto, and to uses thereof. Description of the Related Art Random screening of molecules for possible activity as therapeutic agents has occurred for many years and resulted in a number of important drug discoveries. While advances in molecular biology and 10 computational chemistry have led to increased interest in what has been termed "rational drug design," such techniques have not proven as fast or reliable as initially predicted. Thus, in recent years there has been a renewed interest and return to random drug screening. To this end, particular strides having been made in new technologies based on the development of 15 combinatorial chemistry libraries, and the screening of such libraries in search for biologically active members. In general, combinatorial chemistry libraries are simply a collection of molecules. Such libraries vary by the chemical species within the library, as well as the methods employed to both generate the library members 20 and identify which members interact with biological targets of interest. While this field is still young, methods for generating and screening libraries have already become quite diverse and sophisticated. For example, a recent review of various combinatorial chemical libraries has identified a number of such techniques (Dolle, J. Com. Chem., 2(3): 383-433, 2000), including the 25 use of both tagged and untagged library members (Janda, Proc. Natl. Acad. Sci. USA 91:10779-10785, 1994). Initially, combinatorial chemistry libraries were generally limited to members of peptide or nucleotide origin. To this end, the techniques of Houghten et al. illustrate an example of what is termed a "dual-defined 30 iterative" method to assemble soluble combinatorial peptide libraries via split synthesis techniques (Nature (London) 354:84-86, 1991; Biotechniques 13:412-421, 1992; Bioorg. Med. Chem. Lett. 3:405-412, 1993). By this technique, soluble peptide libraries containing tens of millions of members 1 WO 2004/108731 PCT/US2003/017188 have been obtained. Such libraries have been shown to be effective in the identification of opioid peptides, such as methionine- and leucine-enkephalin (Dooley and Houghten, Life Sci. 52, 1509-1517, 1993), and a N-acylated peptide library has been used to identify acetalins, which are potent opioid 5 antagonists (Dooley et al., Proc. Natl. Acad. Sci. USA 90:10811-10815, 1993. More recently, an all D-amino acid opioid peptide library has been constructed and screened for analgesic activity against the mu ("p") opioid receptor (Dooley et al, Science 266:2019-2022, 1994). While combinatorial libraries containing members of peptide and 10 nucleotide origin are of significant value, there is still a need in the art for libraries containing members of different origin. For example, traditional peptide libraries to a large extent merely vary the amino acid sequence to generate library members. While it is well recognized that the secondary structures of peptides are important to biological activity, such peptide libraries 15 do not impart a constrained secondary structure to its library members. To this end, some researchers have cyclized peptides with disulfide bridges in an attempt to provide a more constrained secondary structure (Tumelty et al., J. Chem. Soc. 1067-68, 1994; Eichler et al., Peptide Res. 7:300-306, 1994). However, such cyclized peptides are generally still 20 quite flexible and are poorly bioavailable, and thus have met with only limited success. More recently, non-peptide compounds have been developed which more closely mimic the secondary structure of reverse-turns found in biologically active proteins or peptides. For example, U.S. Pat. No. 5,440,013 25 to Kahn and published PCT WO94/03494 to Kahn both disclose conformationally constrained, non-peptidic compounds, which mimic the three dimensional structure of reverse-turns. While significant advances have been made in the synthesis and identification of conformationally constrained, peptide mimetics, there remains 30 a need in the art for small molecules, which mimic the secondary structure of peptides. There has been also a need in the art for libraries containing such members, as well as techniques for synthesizing and screening the library members against targets of interest, particularly biological targets, to identify bioactive library members. For example U.S. Pat. No. 5,929,237 and its 35 continuation-in-part U.S. Pat. No. 6,013,458 to Kahn also discloses 2 WO 2004/108731 PCT/US2003/017188 conformationally constrained compounds that mimic the secondary structure of reverse-turn regions of biologically active peptides and proteins. The present invention also fulfills these needs, and provides further related advantages by providing confomationally constrained 5 compounds which mimic the secondary structure of the P-strand structures of biologically active peptides and proteins. BRIEF SUMMARY OF THE INVENTION In brief, the present invention is directed to conformationally constrained compounds that mimic the secondary structure of the P-strand 10 structures of biologically active peptides and proteins. This invention also discloses libraries containing such compounds, as well as the synthesis and screening thereof. The compounds of the present invention have the following general structure (I): R 1 W / z R 2 X /B N N y N n R4 Y 15 L (I) wherein A is -(CH)-, -N- or -CH 2 -N-, B is -(C=O)- or -(CH 2 ) m-, W is -(C=O) -Y(C=O)-, -NH(C=O)- or nothing, X is -NH-, -NH(C=O)- or nothing, Y is oxygen or sulfur, Z is oxygen or hydrogen, L is hydrogen, R 5 , -C(O)NHR 3 or its 20 equivalents, n=0 or I and m=1 or 2; R 1 , R 2 , R 3 , R 4 and R 5 are the same or different and independently selected from hydrogen, an amino acid side chain moiety or derivative thereof, the remainder of the molecule, a linker and a solid support, and stereoisomers thereof. In one embodiment of the invention, X is absent, A is -N-, B 25 is -(C=O)-, L is -C(O)NHR 3 , and other groups are as defined above in structure (I), so that the compounds of the invention have the following structure (1'): 3 WO 2004/108731 PCT/US2003/017188 R1 O W /z N R 2 -N NR4 n Y 0 N R H (') Optionally, W is absent and Z is oxygen. In one embodiment of the invention, X is absent, A is -N-, B is -(CH 2 ) m-, L is -C(O)NHR 3 , and other groups are as defined above in 5 connection with structure (I), so that the compounds of the invention have the following structure (1"): R 1 W /z R 2 -N Y N R Y O N R H (i") Optionally, W is absent and Z is oxygen. In one embodiment of the invention, X is -NH-, A is -(CH)-, B 10 is -(CH 2 ) m-, L is -C(O)NHR 3 , and the other groups are as defined in connection with structure (I), so that the compounds of the invention have the following structure (1"'): 4 WO 2004/108731 PCT/US2003/017188 RI w / z R2N m N H NR4 n Y 0 1N R3 H ('") Optionally, when Z is oxygen, then W is absent. In one embodiment of the invention, A is -CH 2 -N-, B is -(CH 2 ) m-, L is -C(O)NHR 3 , and the other groups are as defined above in connection with 5 structure (I), so that the compounds of the invention have the following structure (1""): R1 W R2'R' )m l..._ N Y H ("') Optionally, Y is oxygen, and/or W is absent, and/or Z is oxygen. The present invention is also directed to libraries containing 10 compounds of structures (I), (I)'), (1"), (1'"), and (1"") above, as well as methods for synthesizing such libraries and methods for screening the same to identify biologically active compounds. Compositions containing a compound of this invention in combination with a pharmaceutically acceptable carrier or diluent are also disclosed. 15 These and other aspects of this invention will be apparent upon reference to the following detailed description and the drawings. BRIEF DESCRIPTION OF THE SEVERAL VIEWS OF THE DRAWINGS Figures 1 and 2 illustrate synthetic methodology for preparing libraries of the present invention, and compounds of the present invention. 5 WO 2004/108731 PCT/US2003/017188 Figure 3 illustrates synthetic methodology for preparing a library of the present invention, and compounds of the present invention, as more thoroughly described in Example 9. Figure 4 illustrates synthetic methodology for preparing a library 5 of the present invention, and compounds of the present invention, as more thoroughly described in Example 10. DETAILED DESCRIPTION OF THE INVENTION Conformationally constrained compounds which mimic the secondary structure of P-strand regions of biologically active peptides and 10 proteins are disclosed. Such B-strand mimetic structures have utility over a wide range of fields, including use as diagnostic and therapeutic agents. Libraries containing the P-strand mimetic structures of this invention are also disclosed as well as methods for screening the same to identify biologically active members. 15 In one aspect, the present invention is directed to 1-strand mimetic structures and chemical libraries containing P-strand mimetic structures. The 1-strand mimetic structures of the present invention are useful as bioactive agents, including (but not limited to) use as diagnostic, prophylactic and/or therapeutic agents. The B-strand mimetic structure 20 libraries of this invention are useful in the identification of such bioactive agents. In the practice of the present invention, the libraries may contain from tens to hundreds to thousands (or greater) of individual P-strand mimetic structures (also referred to herein as "members"). In one aspect of the present invention, a P-strand mimetic 25 structure is disclosed having the following structure (I): R1 W w / z. R /B N R4 X-A/ Y , L (I) wherein A is -(CH)-, -N- or -CH 2 -N-, B is -(C=O)- or -(CH 2 )m-, W is -(C=O) 6 WO 2004/108731 PCT/US2003/017188 -Y(C=O)-, -NH(C=O)- or nothing, X is -NH-, -NH(C=O)- or nothing, Y is oxygen or sulfur, Z is oxygen or hydrogen (when Z is hydrogen, then C=Z represents CH 2 ), L is hydrogen, R 5 , -C(O)NHR 3 or its equivalents, n=0 or 1 and m=1 or 2; R 1 , R 2 , R 3 , R 4 and R 5 are the same or different and independently 5 selected from hydrogen, an amino acid side chain moiety or derivative thereof, the remainder of the molecule, a linker and a solid support, and stereoisomers thereof In one aspect of the invention, R 1 , R 2 , R 3 , R 4 and R 5 are independently selected from the group consisting of aminoC 2 - 5 alkyl, 10 guanidineC 2 .salkyl, C 1 - 4 alkylguanidinoC 2 - 5 alkyl, diC 1 - 4 alkylguanidino-C 2 -5alkyl, amidinoC 2 -5alkyl, C 1 - 4 alkylamidinoC 2 -5alkyl, diC 1 - 4 alkylamidinoC 2 -5alkyl, C1. 3 alkoxy, Phenyl, substituted phenyl (where the substituents are independently selected from one or more of amino, amidino, guanidine, hydrazine, amidrazonyl, C1- 4 alkylamino, C 1 -4dialkylamino, halogen, 15 perfluoroC 1 - 4 alkyl, CI- 4 alkyl, C 1 - 3 alkoxy, nitro, carboxy, cyano, sulfuryl, or hydroxyl), benzyl, substituted benzyl (where the substituents on the benzyl are independently selected from one or more of amino, amidino, guanidino, hydrazino, amidrazonyl, C1- 4 alkylamino, C1- 4 dialkylamino, halogen, perfluoroCi- 4 alkyl, C 1 .- 3 alkoxy, nitro, carboxy, cyano, sulfuryl, or hydroxyl), 20 naphthyl, substituted naphthyl (where the substituents are independently selected from one or more of amino, amidino, guanidine, hydrazine, amidrazonyl, C 1 -4alkylamino, C 1 - 4 dialkylamino, halogen, perfluoro C1-4alkyl, C1- 4 alkyl, C1- 3 alkoxy, nitro, carboxy, cyano, sulfuryl, or hydroxyl), bis-phenyl methyl, substituted bis-phenyl methyl(where the substituents are independently 25 selected from one or more of amino, amidino, guanidine, hydrazine, amidrazonyl, C1-4alkylamino, C 1 - 4 dialkylamino, halogen, perfluoro C1-4alkyl, C 1 - 4 alkyl, C1- 3 alkoxy, nitro, carboxy, cyano, sulfuryl, or hydroxyl), pyridyl, substituted pyridyl, (where the substituents are independently selected from one or more of amino amidino, guanidino, hydrazino, amidrazonyl, 30 C 14 alkylamino, C 1 4 dialkylamino, halogen, perfluoro C1- 4 alkyl, C1. 4 alkyl, C1-3alkoxy, nitro, carboxy, cyano, sulfuryl, or hydroxyl), pyridylC 1 - 4 alkyl, substituted pyridylC 1 _ 4 alkyl (where the pyridine substituents are independently selected from one or more of amino, amidino, guanidino, hydrazino, amidrazonyl, C1-4alkylamino, C- 4 dialkylamino, halogen, perfluoro C 1 - 4 alkyl, 35 C 1 - 4 alkyl, C 1 . 3 alkoxy, nitro, carboxy, cyano, sulfuryl, or hydroxyl), pyrimidylCl. 4 alkyl, substituted pyrimidylC 1 - 4 alkyl (where the pyrimidine substituents are 7 WO 2004/108731 PCT/US2003/017188 independently selected from one or more of amino, amidino, guanidine, hydrazine, amidrazonyl, C 1 . 4 alkylamino, CI- 4 dialkylamino, halogen, perfluoroC 1 - 4 alkyl, C1- 4 alkyl, C 1 - 3 alkoxy or nitro, carboxy, cyano, sulfuryl, or hydroxyl), triazin-2-y-C 1 - 4 alkyl, substituted triazin-2-yl-C 1 - 4 alkyl (where the 5 triazine substituents are independently selected from one or more of amino, amidino, guanidine, hydrazine, amidrazonyl, C 1 i- 4 alkylamino, C1- 4 dialkylamino, halogen, perfluoro CI- 4 alkyl, C1-4alkyl, C1- 3 alkoxy, nitro, carboxy, cyano, sulfuryl, or hydroxyl), imidazoC 1 - 4 alkyl, substituted imidazol C1- 4 alkl (where the imidazole substituents are independently selected from one or more of amino, 10 amidino, guanidine, hydrazine, amidrazonyl, Ci- 4 alkylamino, C 1 - 4 dialkylamino, halogen, perfluoro C1- 4 alkyl, C1- 4 alkyl, C1-3alkoxy, nitro, carboxy, cyano, sulfuryl, or hydroxyl), imidazolinylC 1 - 4 alkyl, N-amidinopiperazinyl-N-CO- 4 alkyl, hydroxyC 2 -Salkyl, C 1 - 5 alkylaminoC 2 -salkyl, hydroxyC 2 -5alkyl, C 1 . -salkylaminoC 2 - 5 alkyl, Cl 1 5 dialkylaminoC2-5alkyl, N-amidinopiperidinylC 1 .- 4 alkyl 15 and 4-aminocyclohexylCO- 2 alkyl. In one embodiment, R 1 , R 2 and R 3 are the same or different and represent the remainder of the compound, and R 4 is selected from an amino acid side chain moiety or derivative thereof. In another embodiment, L represents -C(=O)NHR 3 , and R 1 , R 2 and R 3 are the same or different and 20 represent the remainder of the compound or an amino acid side chain moiety or derivative thereof, and R 4 is hydrogen. As used herein, the term "amino acid side chain moiety" represents any amino acid side chain moiety present in naturally occurring proteins including (but not limited to) the naturally occurring amino acid side 25 chain moieties identified in Table 1. Other naturally occurring amino acid side chain moieties of this invention include (but are not limited to) the side chain moieties of 3,5-dibromotyrosine, 3,5-diiodotyrosine, hydroxylysine, y-carboxyglutamate, phosphotyrosine and phosphoserine. In addition, glycosylated amino acid side chains may also be used in the practice of this 30 invention, including (but not limited to) glycosylated threonine, serine and asparagine. 8 WO 2004/108731 PCT/US2003/017188 Table 1 Amino Acid Side Chain Moieties Amino Acid Side Chain Moiety Amino Acid -H Glycine -CH 3 Alanine -CH(CH 3 ) 2 Valine -CH 2 CH(CH 3 ) 2 Leucine -CH(CH 3 )CH 2 CH 3 Isoleucine - (CH 2 ) 4 NH 3 + Lysine - (CH 2 ) 3 NHC(NH 2 )NH 2 Arginine --CH 2 N Histidine N NH -CH 2 COO~ Aspartic acid -CH 2 CH 2 COO Glutamic acid -CH 2 CONH 2 Asparagine -CH 2 CH 2 CONH 2 Glutamine Phenylalanine -CH 2 0 Tyrosine -CH 2 K-O)OH 2Tryptophan N H -CH 2 SH Cysteine -CH 2 CH 2 SCH 3 Methionine -CH 2 OH Serine -CH(OH)CH 3 Threonine -HN Proline N- Hydroxyproline OH 9 WO 2004/108731 PCT/US2003/017188 In addition to naturally occurring amino acid side chain moieties, the amino acid side chain moieties of the present invention also include various derivatives thereof. As used herein, a "derivative" of an amino acid side chain moiety includes modifications and/or variations to naturally 5 occurring amino acid side chain moieties. For example, the amino acid side chain moieties of alanine, valine, leucine, isoleucine and phenylalanine may generally be classified as lower chain alkyl, aryl, or arylalkyl moieties. Derivatives of amino acid side chain moieties include other straight chain or branched, cyclic or noncyclic, substituted or unsubstituted, saturated or 10 unsaturated lower chain alkyl, aryl or arylalkyl moieties. As used herein, the terms "remainder of the compound" and "remainder of the molecule" are used to mean any moiety, agent, compound, support, molecule, linker, amino acid, peptide or protein covalently attached to the P-strand mimetic structure. The attachment is preferably at either the R 1 15 and/or R 2 and/or R 3 positions. This term also includes amino acid side chain moieties and derivatives thereof. As used herein, "lower chain alkyl moieties" contain from 1-12 carbon atoms, "lower chain aryl moieties" contain from 6-12 carbon atoms and "lower chain aralkyl moieties" contain from 7-12 carbon atoms. Thus, in one 20 embodiment, the amino acid side chain derivative is selected from a C1-. 12 alkyl, a C 6 - 12 aryl and a C 7 12 arylalkyl, and in a more preferred embodiment, from a C1-7 alkyl, a C 6 - 10 aryl and a C 7 - 11 arylalkyl. Amino side chain derivatives of this invention further include substituted derivatives of lower chain alkyl, aryl, and arylalkyl moieties, wherein 25 the substituent is selected from (but are not limited to) one or more of the following chemical moieties: -OH, -OR, -COOH, -COOR, -CONH 2 , -NH 2 , -NHR, -NRR, -SH, -SR, -SO 2 R, -SO 2 H, -SOR and halogen (including F, CI, Br and I), wherein each occurrence of R is independently selected from straight chain or branched, cyclic or 30 noncyclic, substituted or unsubstituted, saturated or unsaturated lower chain alkyl, aryl and aralkyl moieties. In one aspect the substituent has less than 18 carbon atoms. Moreover, cyclic lower chain alkyl, aryl and arylalkyl moieties of this invention include naphthalene, as well as heterocyclic compounds such as thiophene, pyrrole, furan, imidazole, oxazole, thiazole, pyrazole, 3-pyrroline, 35 pyrrolidine, pyridine, pyrimidine, purine, quinoline, isoquinoline and carbazole. Amino acid side chain derivatives further include heteroalkyl derivatives of the 10 WO 2004/108731 PCT/US2003/017188 alkyl portion of the lower chain alkyl and aralkyl moieties, including (but not limited to) alkyl and aralkyl phosphonates and silanes. In one aspect of he invention, R 1 , R 2 and R 3 moieties are selected from -OH, -OR, -COR, -COOR, -CONH 2 , -CONR, -CONRR, -NH 2 , 5 NHR,-NRR, -SO 2 R and -COSR, wherein each occurrence of R is as defined above. In a further embodiment, and in addition to being an amino acid side chain moiety or derivative thereof (or the remainder of the compound in the case of R 1 , R 2 and R 3 ), R 1 , R 2 or R 3 may be a linker facilitating the linkage 10 of the compound to another moiety or compound. For example, the compounds of this invention may be linked to one or more known compounds, such as biotin, for use in diagnostic or screening assay. Furthermore, R 1 , R 2 or R 3 may be a linker joining the compound to a solid support (such as a support used in solid phase peptide synthesis) or alternatively, may be the 15 support itself. In this embodiment, linkage to another moiety or compound, or to a solid support, is preferable at the R 1 , R 2 or R 3 position, and more preferably at the R 3 position. In the embodiment wherein X is absent, A is N, B is -(C=O)- and L is -C(O)NHR 3 , the -strand compounds of this invention have the following 20 structure (1'): R1 0 W / z R 2 -N R4 N R 4 Y 0 N H (I') wherein R 1 , R 2 , R 3 , R 4 , W, Y, Z and n are as defined above. In a preferred embodiment, R 2 and R 3 represent the remainder of the compound, R 1 and R 4 are selected from amino acid side chain moieties. 25 In the embodiment wherein X is absent, A is N, B is -(CH 2 ) m- and L is -C(O)NHR 3 , the -strand mimetic structures of this invention include the following structure (1"): 11 WO 2004/108731 PCT/US2003/017188 R, w I z R 2 -N R4 N Y n O N/R3 0 N H (I") wherein Ri, R 2 , R 3 , R 4 , W, Y, Z, m and n are as defined above. In a preferred embodiment, R 2 and R 3 represent the remainder of the compound, and Ri and R 4 are selected from amino acid side chain moieties. 5 In a more specific embodiment wherein X is -NH-, A is -(CH)-, and B is -(CH 2 ) m- and L is -C(O)NHR 3 , the P-strand mimetic structure has the following structure (1"'): R 1 W / z m N R2,-N -, H N R4 n y H (j"') wherein R 1 , R 2 , R 3 , R 4 , W, Y, Z, m and n are as defined above. 10 In a more specific embodiment wherein A is -CH 2 -N-, B is.-(CH 2 ) m- and L is -C(O)NHR 3 , the compounds of this invention have the following structure (I""): 12 WO 2004/108731 PCT/US2003/017188 X ( . ) m RR2w RKR4 N YR 3 Optionally wherein R 1 , R 2 , R 3 , R 4 , W, X, Y, Z, m and n are as defined above, W is absent, Z is oxygen, and Y is oxygen. The 1-strand mimetic structures of the present invention may be 5 prepared by utilizing appropriate starting component molecules (hereinafter referred to as "component pieces"). Briefly, in the synthesis of 3-strand mimetic structures having structure (1'), first and second component pieces are coupled to form a combined first-second intermediate, and if necessary, third and/or fourth component pieces are coupled to form a combined third-fourth 10 intermediate (or, if commercially available, a single third intermediate may be used), the combined first-second intermediate and third-fourth intermediate (or third intermediate) are then coupled to provide a first-second-third-fourth intermediate (or first-second-third intermediate) which is cyclized to yield the 3 strand mimetic structures of this invention. Alternatively, the p-strand mimetic 15 structures of structure (1') may be prepared by sequential coupling of the individual component pieces either stepwise in solution or by solid phase synthesis as commonly practiced in solid phase peptide synthesis. Within the context of the present invention, a "first component piece" has the following structure 1: RO " OR B .R 2 20 BAR2 wherein R 2 , A and B are as defined above, and R is a protective group suitable for use in peptide synthesis. Suitable R groups include alkyl groups and, in a preferred embodiment, R is a methyl group. Such first component pieces may 13 WO 2004/108731 PCT/US2003/017188 be readily synthesized by reductive amination by mating CH(OR) 2 -(CH 2 )m CHO with H 2 N-R 2 , or by displacement from CH(OR) 2 -(CH 2 )m-Br. A "second component piece" of this invention has the following structure 2: O II XC R4 P-N )n H L 5 where L and R 4 are as defined above, P is an amino protective group suitable for use in peptide synthesis, and X represents the leaving group of the activated carboxylic acid group. Preferred protective groups include t-butyl dimethylsilyl (TBDMS), BOC, FMOC, and Alloc(allyloxycarbonyl). When L 10 is -C(O)NHR 3 , -NHR 3 may be an carboxyl protective group. N-Protected amino acids are commercially available, for example, FMOC amino acids are available from a variety of sources. The conversion of these N-protected amino acids to the second component pieces of this invention may be readily achieved by activation of the carboxylic acid group of the N-protected amino 15 acid. Suitable activated carboxylic acid groups include acid halides where X is a halide such as chloride or bromide, acid anhydrides where X is an acyl group such as acetyl, reactive esters such as an N-hydroxysuccinimide esters and pentafluorophenyl esters, and other activated intermediates such as the active intermediate formed in a coupling reaction using a carbodiimide such as 20 dicyclohexylcarbodiimide (DCC). In the case of the azido derivative of an amino acid serving as the second component piece, such compounds may be prepared from the corresponding amino acid by the reaction disclosed by Zaloom et al. (J. Org. Chem. 46:5173-76, 1981). 25 A "third component piece" of this invention has the following structure 3: R 1 -NH 2 or R 3 -NH 2 14 WO 2004/108731 PCT/US2003/017188 where R 1 and R 3 are as defined above. Suitable third component pieces are commercially available from a variety of sources, or may be readily prepared by standard organic synthetic techniques commonly utilized for the synthesis of primary amines. 5 More specifically, the P-strand mimetic structures of this invention of structure (1') are synthesized by reacting a first component piece with a second component piece to yield a combined first-second intermediate, followed by either reacting the combined first-second intermediate with third component pieces sequentially, or third and fourth component pieces, to 10 provide a combined first-second-third-fourth intermediate, and then cyclizing this intermediate to yield the P-strand mimetic structure. The general synthesis of a p-strand mimetic structure having structure I' may be accomplished by the following technique. A first component piece 1 is coupled with a second component piece 2 by using a 15 coupling reagent such as phosgene to yield, after N-deprotection, a combined first-second intermediate 1-2 as illustrated below: 0 OR 0 RO OR 4 OR B R4 BX RR 4 N B"A 2 :XX R A 1YL L S Y L wherein, A, B, L, R, R 2 , R 4 , P, X and n are as defined above. X 2 C(=S) is an example of a coupling agent, and other type of coupling agents can be 20 employed. The syntheses of representative component pieces of this invention are described in the Examples. The P-strand mimetic compounds of structures (1") through (I"') may be made by techniques analogous to the modular component synthesis disclosed above, but with appropriate modifications to the component pieces. 25 In another aspect of this invention, libraries containing the P3 strand mimetic structures of the present invention are disclosed. Once assembled, the libraries of the present invention may be screened to identify individual members having bioactivity. Such screening of the libraries for bioactive members may involve; for example, evaluating the binding activity of 30 the members of the library or evaluating the effect the library members have on 15 WO 2004/108731 PCT/US2003/017188 a functional assay. Screening is normally accomplished by contacting the library members (or a subset of library members) with a target of interest, such as, for example, an antibody, enzyme, receptor or cell line. Library members that are capable of interacting with the target of interest are referred to herein 5 as "bioactive library members" or "bioactive mimetics". For example, a bioactive mimetic may be a library member which is capable of binding to an antibody or receptor, which is capable of inhibiting an enzyme, or which is capable of eliciting or antagonizing a functional response associated, for example, with a cell line. In other words, the screening of the libraries of the 10 present invention determines which library members are capable of interacting with one or more specific biological targets of interest. When interaction does occur, the interacting bioactive mimetic (or mimetics) may be identified from the library members. The identification of a single (or limited number) of bioactive mimetic(s) from the library yields -strand mimetic structures which 15 are themselves biologically active, and thus useful as diagnostic, prophylactic or therapeutic agents, and may further be used to significantly advance identification of lead compounds in these fields. Synthesis of the peptide mimetics of the library of the present invention may be accomplished using known peptide synthesis techniques, in 20 combination with the first, second, third, and optionally fourth component pieces of this invention. More specifically, any amino acid sequence may be added to the N-terminal and/or C-terminal of the conformationally constrained compound. To this end, the mimetics may be synthesized on a solid support (such as PAM resin) by known techniques (see, e.g., John M. Stewart and 25 Janis D. Young, Solid Phase Peptide Synthesis, 1984, Pierce Chemical Comp., Rockford, Ill.) or on a silyl-linked resin by alcohol attachment (see Randolph et al., J. Am Chem. Soc. 117:5712-14, 1995). In addition, a combination of both solution and solid phase synthesis techniques may be utilized to synthesize the peptide mimetics of this 30 invention. For example, a solid support may be utilized to synthesize the linear peptide sequence up to the point that the conformationally constrained 1-strand is added to the sequence. A suitable conformationally constrained 3 strand mimetic structure which has been previously synthesized by solution synthesis techniques may then be added as the next "amino acid" to the solid 35 phase synthesis (i.e., the conformationally constrained P-strand mimetic, which has both an N-terminus and a C-terminus, may be utilized as the next amino 16 WO 2004/108731 PCT/US2003/017188 acid to be added to the linear peptide). Upon incorporation of the conformationally constrained p-strand mimetic structure into the sequence, additional amino acids may then be added to complete the peptide bound to the solid support. Alternatively, the linear N-terminus and C-terminus 5 protected peptide sequences may be synthesized on a solid support, removed from the support, and then coupled to the conformationally constrained P-strand mimetic structures in solution using known solution coupling techniques. In another aspect of this invention, methods for constructing the 10 libraries are disclosed. Traditional combinatorial chemistry techniques (see, e.g., Gallop et al., J. Med. Chem. 37:1233-1251, 1994) permit a vast number of compounds to be rapidly prepared by the sequential combination of reagents to a basic molecular scaffold. Combinatorial techniques have been used to construct peptide libraries derived from the naturally occurring amino acids. 15 For example, by taking 20 mixtures of 20 suitably protected and different amino acids and coupling each with one of the 20 amino acids, a library of 400 (i.e., 202) dipeptides is created. Repeating the procedure seven times results in the preparation of a peptide library comprised of about 26 billion (i.e., 208) octapeptides. 20 In a further aspect of this invention, methods for screening the libraries for bioactivity and isolating bioactive library members are disclosed. The libraries of the present invention may be screened for bioactivity by a variety of techniques and methods. Generally, the screening assay may be performed by (1) contacting a library with a biological target of interest, such as 25 a receptor, and allowing binding to occur between the mimetics of the library and the target, and (2) detecting the binding event by an appropriate assay, such as by the calorimetric assay disclosed by Lam et al. (Nature 354:82-84, 1991) or Griminski et al. (Biotechnology 12:1008-1011, 1994) (both of which are incorporated herein by reference). In a preferred embodiment, the library 30 members are in solution and the target is immobilized on a solid phase. Alternatively, the library may be immobilized on a solid phase and may be probed by contacting it with the target in solution. The synthesis of the peptide mimetics of a library of the present invention may be accomplished using the general scheme for preparing a 35 p-strand mimetics library as shown in Figure 1. The synthesis of selected peptide mimetics of the bicyclic template libraries of the present invention was 17 WO 2004/108731 PCT/US2003/017188 accomplished using a FlexChem Reactor Block which has a 96 well plate. In the above scheme 'Pol' represents 2-chlorotrityl chloride resin (Novabiochem) and a detailed procedure is provided below. Step 1 The 2-chlorotrityl chloride resin (1 mmol/g) and a 5 solution Fmoc-R 1 -Amino Acid (1.5 equiv.) and DIEA (2 equiv.) in DCE were placed in a 96 well Robinson block (Flexchem). The reaction mixture was shaken for 12 hours at room temperature. The resin washed with DMF, MeOH, and the DCM. Step2 To the resin swollen by DMF before reaction was 10 added 25% piperidine in DMF. Thereafter, the reaction mixture was shaken for 30 min at room temperature. The deprotection step was repeated and the product mixture was washed with DMF, MeOH, and then DCM. A solution of 4-R 2 -amino-2-Fmoc-aminobutyric acid (1.5 equiv.), DIC (1.5 equiv.), and HOBT (1.5 equiv.) in NMP was added to the resin. After the reaction mixture was 15 shaken for 12 hours at room temperature, the resin was washed with DMF, MeOH, and then DCM. Step 3 To the resin swollen by DMF before reaction was added 25% piperidine in DMF. Thereafter, the reaction mixture was shaken for 30 min at room temperature. The deprotection step was repeated and the 20 product mixture was washed with DMF, MeOH, and then DCM. A solution of 2-(9H-fluoren-9-ylmethoxycarbonylamino)-5,5-dimethoxy-pentanoic acid (1.5 equiv.), DIC (1.5 equiv.), and HOBT (1.5 equiv.) in NMP was added to the resin. The reaction mixture was shaken for 12 hours at room temperature, and then the resin was washed with DMF, MeOH, and then DCM. 25 Step 4 To the resin swollen by DMF before reaction was added 25% piperidine in DMF. Thereafter, the reaction mixture was shaken for 30 min at room temperature. The deprotection step was repeated and the product mixture was washed with DMF, MeOH, and then DCM. A solution of commercially available R 3 -acid (1.5 equiv.), DIC (1.5 equiv.), and HOBT 30 (1.5 equiv.) in NMP was added to the resin. The reaction mixture was, shaken for 12 hours at room temperature, and then the resin was washed with DMF, MeOH, and then DCM. Step 5 The resin was treated with formic acid (1.2 mL each well) for 18 hours at room temperature. Thereafter, the resin was removed by 35 filtration, the filtrate was condensed under reduced pressure using SpeedVac 18 WO 2004/108731 PCT/US2003/017188 (Servant) to give the product as oil. These products were diluted with 50% water/acetonitrile and then lyophilized after freezing. Table 2 shows a p-strand mimetics library that may be prepared according to the present invention, of which representative preparation is given 5 in Example 9. Compounds of Table 2 illustrate one aspect of the invention, namely compounds wherein A is -(CH)-, B is -(CH 2 ) m- with m = 1, W is -(C=O)-, X is-NH(C=O)-, Y is oxygen, Z is hydrogen so that C=Z represents CH 2 , L is -C(=O)NHR 3 , n=0, R 4 is hydrogen, and R 1 , R 2 , and R 3 are the same or different and are independently selected from an amino acid side chain 10 moiety or derivative thereof, the remainder of the molecule, a linker and a solid support, and stereoisomers thereof. In various embodiments of this aspect of the invention, R 1 , R 2 , and R 3 are independently selected from relatively low molecular weight moieties, i.e., organic groups having molecular weights of between 15 (methyl) and 1,000 g/mol; and/or at least one of R 1 , R 2 , and R 3 15 represents an amino acid side chain or derivative thereof. For example, in the compounds of Table 2, R 3 represents aspartic acid derivatives. In one aspect, the compounds of the present invention have a molecular weight within the range of about 440 to 750 g/mol, where the compounds of Table 2 provide numerous illustrations of such compounds. 20 19 WO 2004/108731 PCT/US2003/017188 TABLE 2 THE BETA-STRAND MIMETICS LIBRARY O R b N Rc N Ra O 0 0 N OH ON H 0 5 No. Ra R b Rc Mol. M+H Weight 1 Methyl Phenyl 4-Methoxybenzyl 522 522 2 Methyl Phenyl 3,4-CI2-benzyl 547 547 3 Methyl Phenyl 1-Naphthyl 528 528 4 Methyl Phenyl Piperonyl 522 522 5 Methyl Phenyl 2,4,5-Trimethoxyphenyl 568 568 6 Methyl Phenyl 2-Thienylmethyl 498 498 7 Methyl Phenyl 1-Naphthylmethyl 542 542 8 Methyl Phenyl Phenethyl 506 506 9 Methyl Phenyl 3-Methoxyphenyl 508 508 10 Methyl Phenyl N-Benzoylaminoethyl 535 535 11 Methyl Phenyl Benzyl 492 492 12 Methyl Phenyl 4-Nitrobenzyl 537 537 13 Isopropyl Phenyl 4-Methoxybenzyl 550 550 14 Isopropyl Phenyl 3,4-CI2-benzyl 575 575 15 Isopropyl Phenyl 1-Naphthyl 556 556 16 Isopropyl Phenyl Piperonyl 550 550 17 Isopropyl Phenyl 2,4,5-Trimethoxyphenyl 596 596 18 Isopropyl Phenyl 2-Thienylmethyl 526 526 19 Isopropyl Phenyl 1-Naphthylmethyl 570 570 20 Isopropyl Phenyl Phenethyl 534 534 21 Isopropyl Phenyl 3-Methoxyphenyl 536 536 22 Isopropyl Phenyl N-Benzoylaminoethyl 563 563 23 Isopropyl Phenyl Benzyl 520 520 24 Isopropyl Phenyl 4-Nitrobenzyl 565 565 25 Isobutyl Phenyl 4-Methoxybenzyl 564 564 26 Isobutyl Phenyl 3,4-Cl 2 -benzyl 589 589 20 WO 2004/108731 PCT/US2003/017188 No. R a R b R c Mol. M+H Weight 27 Isobutyl Phenyl 1-Naphthyl 570 570 28 Isobutyl Phenyl Piperonyl 564 564 29 Isobutyl Phenyl 2,4,5-Trimethoxyphenyl 610 610 30 Isobutyl Phenyl 2-Thienylmethyl 540 540 31 Isobutyl Phenyl 1-Naphthylmethyl 584 584 32 Isobutyl Phenyl Phenethyl 548 548 33 Isobutyl Phenyl 3-Methoxyphenyl 550 550 34 Isobutyl Phenyl N-Benzoylaminoethyl 577 577 35 Isobutyl Phenyl Benzyl 534 534 36 Isobutyl Phenyl 4-Nitrobenzyl 579 579 37 Benzyl Phenyl 4-Methoxybenzyl 598 598 38 Benzyl Phenyl 3,4-CI 2 -benzyl 623 623 39 Benzyl Phenyl 1-Naphthyl 604 604 40 Benzyl Phenyl Piperonyl 598 598 41 Benzyl Phenyl 2,4,5-Trimethoxyphenyl 644 644 42 Benzyl Phenyl 2-Thienylmethyl 574 574 43 Benzyl Phenyl 1-Naphthylmethyl 618 618 44 Benzyl Phenyl Phenethyl 582 582 45 Benzyl Phenyl 3-Methoxyphenyl 584 584 46 Benzyl Phenyl N-Benzoylaminoethyl 611 611 47 Benzyl Phenyl Benzyl 568 568 48 Benzyl Phenyl 4-Nitrobenzyl 613 613 49 Methyl Methoxy 4-Methoxybenzyl 476 476 50 Methyl Methoxy 3,4-CI 2 -benzyl 501 501 51 Methyl Methoxy 1-Naphthyl 482 482 52 Methyl Methoxy Piperonyl 476 476 53 Methyl Methoxy 2,4,5-Trimethoxyphenyl 522 522 54 Methyl Methoxy 2-Thienylmethyl 452 452 55 Methyl Methoxy 1-Naphthylmethyl 496 496 56 Methyl Methoxy Phenethyl 460 460 57 Methyl Methoxy 3-Methoxyphenyl 462 462 58 Methyl Methoxy N-Benzoylaminoethyl 489 489 59 Methyl Methoxy Benzyl 446 446 60 Methyl Methoxy 4-Nitrobenzyl 491 491 61 Isopropyl Methoxy 4-Methoxybenzyl 504 504 62 Isopropyl Methoxy 3,4-CI 2 -benzyl 529 529 63 Isopropyl Methoxy 1-Naphthyl 510 510 64 Isopropyl Methoxy Piperonyl 504 504 65 Isopropyl Methoxy 2,4,5-Trimethoxyphenyl 550 550 21 WO 2004/108731 PCT/US2003/017188 No. Ra R b Rc Mol. M+H Weight 66 Isopropyl Methoxy 2-Thienylmethyl 480 480 67 Isopropyl Methoxy 1-Naphthylmethyl 524 524 68 Isopropyl Methoxy Phenethyl 488 488 69 Isopropyl Methoxy 3-Methoxyphenyl 490 490 70 Isopropyl Methoxy N-Benzoylaminoethyl 517 517 71 Isopropyl Methoxy Benzyl 474 474 72 Isopropyl Methoxy 4-Nitrobenzyl 519 519 73 Isobutyl Methoxy 4-Methoxybenzyl 518 518 74 Isobutyl Methoxy 3,4-CI 2 -benzyl 543 543 75 Isobutyl Methoxy 1-Naphthyl 524 524 76 Isobutyl Methoxy Piperonyl 518 518 77 Isobutyl Methoxy 2,4,5-Trimethoxyphenyl 564 564 78 Isobutyl Methoxy 2-Thienylmethyl 494 494 79 Isobutyl Methoxy 1-Naphthylmethyl 538 538 80 Isobutyl Methoxy Phenethyl 502 502 81 Isobutyl Methoxy 3-Methoxyphenyl 504 504 82 Isobutyl Methoxy N-Benzoylaminoethyl 531 531 83 Isobutyl Methoxy Benzyl 488 488 84 Isobutyl Methoxy 4-Nitrobenzyl 533 533 85 Benzyl Methoxy 4-Methoxybenzyl 552 552 86 Benzyl Methoxy 3,4-CI 2 -benzyl 577 577 87 Benzyl Methoxy 1-Naphthyl 558 558 88 Benzyl Methoxy Piperonyl 552 552 89 Benzyl Methoxy 2,4,5-Trimethoxyphenyl 598 598 90 Benzyl Methoxy 2-Thienylmethyl 528 528 91 Benzyl Methoxy 1-Naphthylmethyl 572 572 92 Benzyl Methoxy Phenethyl 536 536 93 Benzyl Methoxy 3-Methoxyphenyl 538 538 94 Benzyl Methoxy N-Benzoylaminoethyl 565 565 95 Benzyl Methoxy Benzyl 522 522 96 Benzyl Methoxy 4-Nitrobenzyl 567 567 97 2-Methylpropyl Phenyl 4-Methoxybenzyl 564 564 98 2-Methylpropyl Phenyl 3,4-CI 2 -benzyl 589 589 99 2-Methylpropyl Phenyl 1-Naphthyl 570 570 100 2-Methylpropyl Phenyl Piperonyl 564 564 101 2-Methylpropyl Phenyl 2,4,5-Trimethoxyphenyl 610 610 102 2-Methylpropyl Phenyl 2-Thienylmethyl 550 550 103 2-Methylpropyl Phenyl 1-Naphthylmethyl 584 584 104 2-Methylpropyl Phenyl Phenethyl 548 548 22 WO 2004/108731 PCT/US2003/017188 No. R a R b R c Mol. M+H Weight 105 2-Methylpropyl Phenyl 3-Methoxyphenyl 550 550 106 2-Methylpropyl Phenyl N-Benzoylaminoethyl 577 577 107 2-Methylpropyl Phenyl Benzyl 534 534 108 2-Methylpropyl Phenyl 4-Nitrobenzyl 579 579 109 Methylthioethyl Phenyl 4-Methoxybenzyl 582 582 110 Methylthioethyl Phenyl 3,4-CI 2 -benzyl 607 607 111 Methylthioethyl Phenyl 1-Naphthyl 588 588 112 Methylthioethyl Phenyl Piperonyl 582 582 113 Methylthioethyl Phenyl 2,4,5-Trimethoxyphenyl 628 628 114 Methylthioethyl Phenyl 2-Thienylmethyl 568 568 115 Methylthioethyl Phenyl 1-Naphthylmethyl 602 602 116 Methylthioethyl Phenyl Phenethyl 566 566 117 Methylthioethyl Phenyl 3-Methoxyphenyl 568 568 118 Methylthioethyl Phenyl N-Benzoylaminoethyl 595 595 119 Methylthioethyl Phenyl Benzyl 552 552 120 Methylthioethyl Phenyl 4-Nitrobenzyl 597 597 121 4-Hydroxybenzyl Phenyl 4-Methoxybenzyl 614 614 122 4-Hydroxybenzyl Phenyl 3,4-CI 2 -benzyl 639 639 123 4-Hydroxybenzyl Phenyl 1-Naphthyl 620 620 124 4-Hydroxybenzyl Phenyl Piperonyl 614 614 125 4-Hydroxybenzyl Phenyl 2,4,5-Trimethoxyphenyl 660 660 126 4-Hydroxybenzyl Phenyl 2-Thienylmethyl 600 600 127 4-Hydroxybenzyl Phenyl 1-Naphthylmethyl 634 634 128 4-Hydroxybenzyl Phenyl Phenethyl 598 598 129 4-Hydroxybenzyl Phenyl 3-Methoxyphenyl 600 600 130 4-Hydroxybenzyl Phenyl N-Benzoylaminoethyl 627 627 131 4-Hydroxybenzyl Phenyl Benzyl 584 584 132 4-Hydroxybenzyl Phenyl 4-Nitrobenzyl 629 629 133 Cyclohexylmethyl Phenyl 4-Methoxybenzyl 604 604 134 Cyclohexylmethyl Phenyl 3,4-CI 2 -benzyl 629 629 135 Cyclohexylmethyl Phenyl 1-Naphthyl 610 610 136 Cyclohexylmethyl Phenyl Piperonyl 604 604 137 Cyclohexylmethyl Phenyl 2,4,5-Trimethoxyphenyl 650 650 138 Cyclohexylmethyl Phenyl 2-Thienylmethyl 590 590 139 Cyclohexylmethyl Phenyl 1-Naphthylmethyl 624 624 140 Cyclohexylmethyl Phenyl Phenethyl 588 588 141 Cyclohexylmethyl Phenyl 3-Methoxyphenyl 590 590 142 Cyclohexylmethyl Phenyl N-Benzoylaminoethyl 617 617 143 Cyclohexylmethyl Phenyl Benzyl 574 574 23 WO 2004/108731 PCT/US2003/017188 No. R a Rb R c Mol. M+H Weight 144 Cyclohexylmethyl Phenyl 4-Nitrobenzyl 619 619 145 2-Methylpropyl Methoxy 4-Methoxybenzyl 518 518 146 2-Methylpropyl Methoxy 3,4-CI 2 -benzyl 543 543 147 2-Methylpropyl Methoxy 1-Naphthyl 524 524 148 2-Methylpropyl Methoxy Piperonyl 518 518 149 2-Methylpropyl Methoxy 2,4,5-Trimethoxyphenyl 564 564 150 2-Methylpropyl Methoxy 2-Tthienylmethyl 504 504 151 2-Methylpropyl Methoxy 1-Naphthylmethyl 538 538 152 2-Methylpropyl Methoxy Phenethyl 502 502 153 2-Methylpropyl Methoxy 3-Methoxyphenyl 504 504 154 2-Methylpropyl Methoxy N-Benzoylaminoethyl 531 531 155 2-Methylpropyl Methoxy Benzyl 488 488 156 2-Methylpropyl Methoxy 4-Nitrobenzyl 533 533 157 Methylthioethyl Methoxy 4-Methoxybenzyl 536 536 158 Methylthioethyl Methoxy 3,4-CI 2 -benzyl 561 561 159 Methylthioethyl Methoxy 1-Naphthyl 542 542 160 Methylthioethyl Methoxy Piperonyl 536 536 161 Methylthioethyl Methoxy 2,4,5-Trimethoxyphenyl 582 582 162 Methylthioethyl Methoxy 2-Tthienylmethyl 522 522 163 Methylthioethyl Methoxy 1-Naphthylmethyl 556 556 164 Methylthioethyl Methoxy Phenethyl 520 520 165 Methylthioethyl Methoxy 3-Methoxyphenyl 522 522 166 Methylthioethyl Methoxy N-Benzoylaminoethyl 549 549 167 Methylthioethyl Methoxy Benzyl 506 506 168 Methylthioethyl Methoxy 4-Nitrobenzyl 551 551 169 4-Hydroxybenzyl Methoxy 4-Methoxybenzyl 568 568 170 4-Hydroxybenzyl Methoxy 3,4-CI 2 -benzyl 593 593 171 4-Hydroxybenzyl Methoxy 1-Naphthyl 574 574 172 4-Hydroxybenzyl Methoxy Piperonyl 568 568 173 4-Hydroxybenzyl Methoxy 2,4,5-Trimethoxyphenyl 614 614 174 4-Hydroxybenzyl Methoxy 2-Tthienylmethyl 554 554 175 4-Hydroxybenzyl Methoxy 1-Naphthylmethyl 588 588 176 4-Hydroxybenzyl Methoxy Phenethyl 552 552 177 4-Hydroxybenzyl Methoxy 3-Methoxyphenyl 554 554 178 4-Hydroxybenzyl Methoxy N-Benzoylaminoethyl 581 581 179 4-Hydroxybenzyl Methoxy Benzyl 538 538 180 4-Hydroxybenzyl Methoxy 4-Nitrobenzyl 583 583 181 Cyclohexylmethyl Methoxy 4-Methoxybenzyl 558 558 182 Cyclohexylmethyl Methoxy 3,4-Cl 2 -benzyl 583 583 24 WO 2004/108731 PCT/US2003/017188 No. Ra R b Rc Mol. M+H Weight 183 Cyclohexylmethyl Methoxy 1-Naphthyl 54 564 184 Cyclohexylmethyl Methoxy Piperonyl 558 558 185 Cyclohexylmethyl Methoxy 2,4,5-Trimethoxyphenyl 604 604 186 Cyclohexylmethyl Methoxy 2-Thienylmethyl 544 544 187 Cyclohexylmethyl Methoxy 1-Naphthylmethyl 578 578 188 Cyclohexylmethyl Methoxy Phenethyl 542 542 189 Cyclohexylmethyl Methoxy 3-Methoxyphenyl 544 544 190 Cyclohexylmethyl Methoxy N-Benzoylaminoethyl 571 571 191 Cyclohexylmethyl Methoxy Benzyl 528 528 192 Cyclohexylmethyl Methoxy 4-Nitrobenzyl 573 573 193 Methyl Phenyl 4-Methoxybenzyl 521 521 194 Methyl Phenyl 3,4-CI 2 -benzyl 546 546 195 Methyl Phenyl 1-Naphthyl 527 527 196 Methyl Phenyl Piperonyl 521 521 197 Methyl Phenyl 2,4,5-Trimethoxyphenyl 567 567 198 Methyl Phenyl 3-Hydroxybenzyl 507 507 199 Methyl Phenyl 1-Naphthylmethyl 541 541 200 Methyl Phenyl Phenethyl 505 505 201 Methyl Phenyl 3-Methoxyphenyl 507 507 202 Methyl Phenyl N-Benzoylaminoethyl 534 534 203 Methyl Phenyl Benzyl 491 491 204 Methyl Phenyl 4-Nitrobenzyl 536 536 205 Isopropyl Phenyl 4-Methoxybenzyl 549 549 206 Isopropyl Phenyl 3,4-CI 2 -benzyl 574 574 207 Isopropyl Phenyl 1-Naphthyl 555 555 208 Isopropyl Phenyl Piperonyl 549 549 209 Isopropyl Phenyl 2,4,5-Trimethoxyphenyl 595 595 210 Isopropyl Phenyl 3-Hydroxybenzyl 535 535 211 Isopropyl Phenyl 1-Naphthylmethyl 569 569 212 Isopropyl Phenyl Phenethyl 533 533 213 Isopropyl Phenyl 3-Methoxyphenyl 535 535 214 Isopropyl Phenyl N-Benzoylaminoethyl 562 562 215 Isopropyl Phenyl Benzyl 519 519 216 Isopropyl Phenyl 4-Nitrobenzyl 564 564 217 Isobutyl Phenyl 4-Methoxybenzyl 563 563 218 Isobutyl Phenyl 3,4-CI 2 -benzyl 588 588 219 Isobutyl Phenyl 1-Naphthyl 569 569 220 Isobutyl Phenyl Piperonyl 563 563 221 Isobutyl Phenyl 2,4,5-Trimethoxyphenyl 609 609 25 WO 2004/108731 PCT/US2003/017188 No. R a Rb Rc Mol. M+H Weight 222 Isobutyl Phenyl 3-Hydroxybenzyl 549 549 223 Isobutyl Phenyl 1-Naphthylmethyl 583 583 224 Isobutyl Phenyl Phenethyl 547 547 225 Isobutyl Phenyl 3-Methoxyphenyl 549 549 226 Isobutyl Phenyl N-Benzoylaminoethyl 576 576 227 Isobutyl Phenyl Benzyl 533 533 228 Isobutyl Phenyl 4-Nitrobenzyl 578 578 229 Benzyl Phenyl 4-Methoxybenzyl 597 597 230 Benzyl Phenyl 3,4-CI2-benzyl 622 622 231 Benzyl Phenyl 1-Naphthyl 603 603 232 Benzyl Phenyl Piperonyl 597 597 233 Benzyl Phenyl 2,4,5-Trimethoxyphenyl 643 643 234 Benzyl Phenyl 3-Hydroxybenzyl 583 583 235 Benzyl Phenyl 1-Naphthylmethyl 617 617 236 Benzyl Phenyl Phenethyl 581 581 237 Benzyl Phenyl 3-Methoxyphenyl 583 583 238 Benzyl Phenyl N-Benzoylaminoethyl 610 610 239 Benzyl Phenyl Benzyl 567 567 240 Benzyl Phenyl 4-Nitrobenzyl 612 612 241 2-Methylpropyl Phenyl 4-Methoxybenzyl 563 563 242 2-Methylpropyl Phenyl 3,4-Cl 2 -benzyl 588 588 243 2-Methylpropyl Phenyl 1-Naphthyl 569 569 244 2-Methylpropyl Phenyl Piperonyl 563 563 245 2-Methylpropyl Phenyl 2,4,5-Trimethoxyphenyl 609 609 246 2-Methylpropyl Phenyl 3-Hydroxybenzyl 549 549 247 2-Methylpropyl Phenyl 1-Naphthylmethyl 583 583 248 2-Methylpropyl Phenyl Phenethyl 547 547 249 2-Methylpropyl Phenyl 3-Methoxyphenyl 549 549 250 2-Methylpropyl Phenyl N-Benzoylaminoethyl 576 576 251 2-Methylpropyl Phenyl Benzyl 533 533 252 2-Methylpropyl Phenyl 4-Nitrobenzyl 578 578 253 Methylthioethyl Phenyl 4-Methoxybenzyl 581 581 254 Methylthioethyl Phenyl 3,4-CI 2 -benzyl 606 606 255 Methylthioethyl Phenyl 1-Naphthyl 587 587 256 Methylthioethyl Phenyl Piperonyl 581 581 257 Methylthioethyl Phenyl 2,4,5-Trimethoxyphenyl 627 627 258 Methylthioethyl Phenyl 3-Hydroxybenzyl 567 567 259 Methylthioethyl Phenyl 1-Naphthylmethyl 601 601 260 Methylthioethyl Phenyl Phenethyl 565 565 26 WO 2004/108731 PCT/US2003/017188 No. R a R R c Mol. M+H Weight 261 Methylthioethyl Phenyl 3-Methoxyphenyl 567 567 262 Methylthioethyl Phenyl N-Benzoylaminoethyl 594 594 263 Methylthioethyl Phenyl Benzyl 551 551 264 Methylthioethyl Phenyl 4-Nitrobenzyl 596 596 265 4-Hydroxybenzyl Phenyl 4-Methoxybenzyl 613 613 266 4-Hydroxybenzyl Phenyl 3,4-Cl2-benzyl 638 638 267 4-Hydroxybenzyl Phenyl 1-Naphthyl 619 619 268 4-Hydroxybenzyl Phenyl Piperonyl 613 613 269 4-Hydroxybenzyl Phenyl 2,4,5-Trimethoxyphenyl 659 659 270 4-Hydroxybenzyl Phenyl 3-Hydroxybenzyl 599 599 271 4-Hydroxybenzyl Phenyl 1-Naphthylmethyl 633 633 272 4-Hydroxybenzyl Phenyl Phenethyl 597 597 273 4-Hydroxybenzyl Phenyl 3-Methoxyphenyl 599 599 274 4-Hydroxybenzyl Phenyl N-Benzoylaminoethyl 626 626 275 4-Hydroxybenzyl Phenyl Benzyl 583 583 276 4-Hydroxybenzyl Phenyl 4-Nitrobenzyl 628 628 277 Cyclohexylmethyl Phenyl 4-Methoxybenzyl 603 603 278 Cyclohexylmethyl Phenyl 3,4-CI 2 -benzyl 628 628 279 Cyclohexylmethyl Phenyl 1-Naphthyl 609 609 280 Cyclohexylmethyl Phenyl Piperonyl 603 603 281 Cyclohexylmethyl Phenyl 2,4,5-Trimethoxyphenyl 649 649 282 Cyclohexylmethyl Phenyl 3-Hydroxybenzyl 589 589 283 Cyclohexylmethyl Phenyl 1-Naphthylmethyl 623 623 284 Cyclohexylmethyl Phenyl Phenethyl 587 587 285 Cyclohexylmethyl Phenyl 3-Methoxyphenyl 589 589 286 Cyclohexylmethyl Phenyl N-Benzoylaminoethyl 616 616 287 Cyclohexylmethyl Phenyl Benzyl 573 573 288 Cyclohexylmethyl Phenyl 4-Nitrobenzyl 618 618 289 Methyl Benzyloxy 4-Methoxybenzyl 553 553 290 Methyl Benzyloxy 3,4-CI 2 -benzyl 577 577 291 Methyl Benzyloxy 1-Naphthyl 559 559 292 Methyl Benzyloxy Piperonyl 553 553 293 Methyl Benzyloxy 2,4,5-Trimethoxyphenyl 599 599 294 Methyl Benzyloxy 2-Thienylmethyl 539 539 295 Methyl Benzyloxy 1-Naphthylmethyl 573 573 296 Methyl Benzyloxy Phenethyl 537 537 297 Methyl Benzyloxy 3-Methoxyphenyl 539 539 298 Methyl Benzyloxy N-Benzoylaminoethyl 566 566 299 Methyl Benzyloxy Benzyl 523 523 27 WO 2004/108731 PCT/US2003/017188 No. Ra R b Rc Mol. M+H Weight 300 Methyl Benzyloxy 4-Nitrobenzyl 568 568 301 Isopropyl Benzyloxy 4-Methoxybenzyl 581 581 302 Isopropyl Benzyloxy 3,4-CI2-benzyl 605 605 303 Isopropyl Benzyloxy 1-Naphthyl 587 587 304 Isopropyl Benzyloxy Piperonyl 581 581 305 Isopropyl Benzyloxy 2,4,5-Trimethoxyphenyl 627 627 306 Isopropyl Benzyloxy 2-Thienylmethyl 567 567 307 Isopropyl Benzyloxy 1-Naphthylmethyl 601 601 308 Isopropyl Benzyloxy Phenethyl 565 565 309 Isopropyl Benzyloxy 3-Methoxyphenyl 567 567 310 Isopropyl Benzyloxy N-Benzoylaminoethyl 594 594 311 Isopropyl Benzyloxy Benzyl 551 551 312 Isopropyl Benzyloxy 4-Nitrobenzyl 596 596 313 Isobutyl Benzyloxy 4-Methoxybenzyl 595 595 314 Isobutyl Benzyloxy 3,4-CI 2 -benzyl 620 620 315 Isobutyl Benzyloxy 1-Naphthyl 601 601 316 Isobutyl Benzyloxy Piperonyl 595 595 317 Isobutyl Benzyloxy 2,4,5-Trimethoxyphenyl 641 641 318 Isobutyl Benzyloxy 2-Thienylmethyl 581 581 319 Isobutyl Benzyloxy 1-Naphthylmethyl 615 615 320 Isobutyl Benzyloxy Phenethyl 579 579 321 Isobutyl Benzyloxy 3-Methoxyphenyl 581 581 322 Isobutyl Benzyloxy N-Benzoylaminoethyl 608 608 323 Isobutyl Benzyloxy Benzyl 565 565 324 Isobutyl Benzyloxy 4-Nitrobenzyl 610 610 325 Benzyl Benzyloxy 4-Methoxybenzyl 629 629 326 Benzyl Benzyloxy 3,4-Cl 2 -benzyl 654 654 327 Benzyl Benzyloxy 1-Naphthyl 635 635 328 Benzyl Benzyloxy Piperonyl 629 629 329 Benzyl Benzyloxy 2,4,5-Trimethoxyphenyl 675 675 330 Benzyl Benzyloxy 2-Thienylmethyl 615 615 331 Benzyl Benzyloxy 1-Naphthylmethyl 649 649 332 Benzyl Benzyloxy Phenethyl 613 613 333 Benzyl Benzyloxy 3-Methoxyphenyl 615 615 334 Benzyl Benzyloxy N-Benzoylaminoethyl 642 642 335 Benzyl Benzyloxy Benzyl 599 599 336 Benzyl Benzyloxy 4-Nitrobenzyl 644 644 337 2-Methylpropyl Benzyloxy 4-Methoxybenzyl 595 595 338 2-Methylpropyl Benzyloxy 3,4-Cl 2 -benzyl 620 620 28 WO 2004/108731 PCT/US2003/017188 No. Ra R b Rc Mol. M+H Weight 339 2-Methylpropyl Benzyloxy 1-Naphthyl 601 601 340 2-Methylpropyl Benzyloxy Piperonyl 595 595 341 2-Methylpropyl Benzyloxy 2,4,5-Trimethoxyphenyl 641 641 342 2-Methylpropyl Benzyloxy 2-Thienylmethyl 581 581 343 2-Methylpropyl Benzyloxy 1-Naphthylmethyl 615 615 344 2-Methylpropyl Benzyloxy Phenethyl 579 579 345 2-Methylpropyl Benzyloxy 3-Methoxyphenyl 581 581 346 2-Methylpropyl Benzyloxy N-Benzoylaminoethyl 608 608 347 2-Methylpropyl Benzyloxy Benzyl 565 565 348 2-Methylpropyl Benzyloxy 4-Nitrobenzyl 610 610 349 Methylthioethyl Benzyloxy 4-Methoxybenzyl 613 613 350 Methylthioethyl Benzyloxy 3,4-CI 2 -benzyl 638 638 351 Methylthioethyl Benzyloxy 1-Naphthyl 619 619 352 Methylthioethyl Benzyloxy Piperonyl 613 613 353 Methylthioethyl Benzyloxy 2,4,5-Trimethoxyphenyl 659 659 354 Methylthioethyl Benzyloxy 2-Tthienylmethyl 599 599 355 Methylthioethyl Benzyloxy 1-Naphthylmethyl 633 633 356 Methylthioethyl Benzyloxy Phenethyl 597 597 357 Methylthioethyl Benzyloxy 3-Methoxyphenyl 599 599 358 Methylthioethyl Benzyloxy N-Benzoylaminoethyl 626 626 359 Methylthioethyl Benzyloxy Benzyl 583 583 360 Methylthioethyl Benzyloxy 4-Nitrobenzyl 628 628 361 4-Hydroxybenzyl Benzyloxy 4-Methoxybenzyl 645 645 362 4-Hydroxybenzyl Benzyloxy 3,4-CI2-benzyl 670 670 363 4-Hydroxybenzyl Benzyloxy 1-Naphthyl 651 651 364 4-Hydroxybenzyl Benzyloxy Piperonyl 645 645 365 4-Hydroxybenzyl Benzyloxy 2,4,5-Trimethoxyphenyl 691 691 366 4-Hydroxybenzyl Benzyloxy 2-Thienylmethyl 631 631 367 4-Hydroxybenzyl Benzyloxy 1-Naphthylmethyl 665 665 368 4-Hydroxybenzyl Benzyloxy Phenethyl 629 629 369 4-Hydroxybenzyl Benzyloxy 3-Methoxyphenyl 631 631 370 4-Hydroxybenzyl Benzyloxy N-Benzoylaminoethyl 658 658 371 4-Hydroxybenzyl Benzyloxy Benzyl 615 615 372 4-Hydroxybenzyl Benzyloxy 4-Nitrobenzyl 660 660 373 Cyclohexylmethyl Benzyloxy 4-Methoxybenzyl 635 635 374 Cyclohexylmethyl Benzyloxy 3,4-CI 2 -benzyl 660 660 375 Cyclohexylmethyl Benzyloxy 1-Naphthyl 641 641 376 Cyclohexylmethyl Benzyloxy Piperonyl 635 635 377 Cyclohexylmethyl Benzyloxy 2,4,5-Trimethoxyphenyl 681 681 29 WO 2004/108731 PCT/US2003/017188 No. Ra Rb Rc Mol. M+H Weight 378 Cyclohexylmethyl Benzyloxy 2-Thienylmethyl 621 621 379 Cyclohexylmethyl Benzyloxy 1-Naphthylmethyl 655 655 380 Cyclohexylmethyl Benzyloxy Phenethyl 619 619 381 Cyclohexylmethyl Benzyloxy 3-Methoxyphenyl 621 621 382 Cyclohexylmethyl Benzyloxy N-Benzoylaminoethyl 648 648 383 Cyclohexylmethyl Benzyloxy Benzyl 605 605 384 Cyclohexylmethyl Benzyloxy 4-Nitrobenzyl 650 650 385 Methyl Methoxy Acetoxymethyl 422 422 386 Methyl Methoxy 4-(2,5-CI2pyridyl)methyl 502 502 387 Methyl Methoxy Chromen-2-one-3-methyl 500 500 388 Methyl Methoxy Methoxymethyl 400 400 389 Methyl Methoxy Pyran-2-one-5-methyl 450 450 390 Methyl Methoxy Ethyl 384 384 391 Methyl Methoxy 2-Ethyldecanyl 510 510 392 Methyl Methoxy Pyrazine-2-methyl 434 434 393 Methyl Methoxy 4-Pyridylmethyl 433 433 394 Methyl Methoxy 1-Butenyl 410 410 395 Methyl Methoxy 2-Nitro-5-Chlorophenyl 511 511 396 Methyl Methoxy Cyanomethyl 395 395 397 Isopropyl Methoxy Acetoxymethyl 450 450 398 Isopropyl Methoxy 4-(2,5-Cl2pyridyl)methyl 530 530 399 Isopropyl Methoxy Chromen-2-one-3-methyl 528 528 400 Isopropyl Methoxy Methoxymethyl 428 428 401 Isopropyl Methoxy Pyran-2-one-5-methyl 478 478 402 Isopropyl Methoxy Ethyl 412 412 403 Isopropyl Methoxy 2-Ethyldecanyl 538 538 404 Isopropyl Methoxy Pyrazine-2-methyl 462 462 405 Isopropyl Methoxy 4-Pyridylmethyl 461 461 406 Isopropyl Methoxy 1-Butenyl 438 438 407 Isopropyl Methoxy 2-Nitro-5-Chlorophenyl 539 539 408 Isopropyl Methoxy Cyanomethyl 423 423 409 Isobutyl Methoxy Acetoxymethyl 464 464 410 Isobutyl Methoxy 4-(2,5-Cl2pyridyl)methyl 544 544 411 Isobutyl Methoxy Chromen-2-one-3-methyl 542 542 412 Isobutyl Methoxy Methoxymethyl 442 442 413 Isobutyl Methoxy Pyran-2-one-5-methyl 492 492 414 Isobutyl Methoxy Ethyl 426 426 415 Isobutyl Methoxy 2-Ethyldecanyl 552 552 416 Isobutyl Methoxy Pyrazine-2-methyl 476 476 30 WO 2004/108731 PCT/US2003/017188 No. Ra R b Rc Mol. M+H Weight 417 Isobutyl Methoxy 4-Pyridylmethyl 475 475 418 Isobutyl Methoxy 1-Butenyl 452 452 419 Isobutyl Methoxy 2-Nitro-5-Chlorophenyl 553 553 420 Isobutyl Methoxy Cyanomethyl 437 437 421 Benzyl Methoxy Acetoxymethyl 498 498 422 Benzyl Methoxy 4-(2,5-CI2pyridyl)methyl 578 578 423 Benzyl Methoxy Chromen-2-one-3-methyl 576 576 424 Benzyl Methoxy Methoxymethyl 476 476 425 Benzyl Methoxy Pyran-2-one-5-methyl 526 526 426 Benzyl Methoxy Ethyl 460 460 427 Benzyl Methoxy 2-Ethyldecanyl 586 586 428 Benzyl Methoxy Pyrazine-2-methyl 510 510 429 Benzyl Methoxy 4-Pyridylmethyl 509 509 430 Benzyl Methoxy 1-Butenyl 486 486 431 Benzyl Methoxy 2-Nitro-5-Chlorophenyl 587 587 432 Benzyl Methoxy Cyanomethyl 471 471 433 2-Methylpropyl Methoxy Acetoxymethyl 464 464 434 2-Methylpropyl Methoxy 4-(2,5-CI2pyridyl)methyl 544 544 435 2-Methylpropyl Methoxy Chromen-2-one-3-methyl 542 542 436 2-Methylpropyl Methoxy Methoxymethyl 442 442 437 2-Methylpropyl Methoxy Pyran-2-one-5-methyl 492 492 438 2-Methylpropyl Methoxy Ethyl 426 426 439 2-Methylpropyl Methoxy 2-Ethyldecanyl 552 552 440 2-Methylpropyl Methoxy Pyrazine-2-methyl 476 476 441 2-Methylpropyl Methoxy 4-Pyridylmethyl 475 475 442 2-Methylpropyl Methoxy 1-Butenyl 452 452 443 2-Methylpropyl Methoxy 2-Nitro-5-Chlorophenyl 553 553 444 2-Methylpropyl Methoxy Cyanomethyl 437 437 445 Methylthioethyl Methoxy Acetoxymethyl 482 482 446 Methylthioethyl Methoxy 4-(2,5-Cl2pyridyl)methyl 562 562 447 Methylthioethyl Methoxy Chromen-2-one-3-methyl 560 560 448 Methylthioethyl Methoxy Methoxymethyl 460 460 449 Methylthioethyl Methoxy Pyran-2-one-5-methyl 510 510 450 Methylthioethyl Methoxy Ethyl 444 444 451 Methylthioethyl Methoxy 2-Ethyldecanyl 570 570 452 Methylthioethyl Methoxy Pyrazine-2-methyl 494 494 453 Methylthioethyl Methoxy 4-Pyridylmethyl 493 493 454 Methylthioethyl Methoxy 1-Butenyl 470 470 455 Methylthioethyl Methoxy 2-Nitro-5-Chlorophenyl 571 571 31 WO 2004/108731 PCT/US2003/017188 No. R a R R c Mol. M+H Weight 456 Methylthioethyl Methoxy Cyanomethyl 455 455 457 4-Hydroxybenzyl Methoxy Acetoxymethyl 514 514 458 4-Hydroxybenzyl Methoxy 4-(2,5-Cl2pyridyl)methyl 594 594 459 4-Hydroxybenzyl Methoxy Chromen-2-one-3-methyl 592 592 460 4-Hydroxybenzyl Methoxy Methoxymethyl 492 492 461 4-Hydroxybenzyl Methoxy Pyran-2-one-5-methyl 542 542 462 4-Hydroxybenzyl Methoxy Ethyl 476 476 463 4-Hydroxybenzyl Methoxy 2-Ethyldecanyl 602 602 464 4-Hydroxybenzyl Methoxy Pyrazine-2-methyl 526 526 465 4-Hydroxybenzyl Methoxy 4-Pyridylmethyl 525 525 466 4-Hydroxybenzyl Methoxy 1-Butenyl 502 502 467 4-Hydroxybenzyl Methoxy 2-Nitro-5-Chlorophenyl 603 603 468 4-Hydroxybenzyl Methoxy Cyanomethyl 487 487 469 2-Hydroxyethyl Methoxy Acetoxymethyl 452 452 470 2-Hydroxyethyl Methoxy 4-(2,5-Cl2pyridyl)methyl 532 532 471 2-Hydroxyethyl Methoxy Chromen-2-one-3-methyl 530 530 472 2-Hydroxyethyl Methoxy Methoxymethyl 430 430 473 2-Hydroxyethyl Methoxy Pyran-2-one-5-methyl 480 480 474 2-Hydroxyethyl Methoxy Ethyl 414 414 475 2-Hydroxyethyl Methoxy 2-Ethyldecanyl 540 540 476 2-Hydroxyethyl Methoxy Pyrazine-2-methyl 464 464 477 2-Hydroxyethyl Methoxy 4-Pyridylmethyl 463 463 478 2-Hydroxyethyl Methoxy 1-Butenyl 440 440 479 2-Hydroxyethyl Methoxy 2-Nitro-5-Chlorophenyl 541 541 480 2-Hydroxyethyl Methoxy Cyanomethyl 425 425 481 Methyl Phenyl 2,4-Pentadienyl 469 469 482 Methyl Phenyl 4-(2,5-Cl2pyridyl)methyl 548 548 483 Methyl Phenyl Chromen-2-one-3-methyl 547 547 484 Methyl Phenyl Methoxymethyl 446 446 485 Methyl Phenyl Pyran-2-one-5-methyl 496 496 486 Methyl Phenyl Ethyl 430 430 487 Methyl Phenyl 2-Ethyldecanyl 501 501 488 Methyl Phenyl Pyrazine-2-methyl 480 480 489 Methyl Phenyl 4-Pyridylmethyl 479 479 490 Methyl Phenyl 1-Butenyl 457 457 491 Methyl Phenyl 2-Nitro-5-Chlorophenyl 558 558 492 Methyl Phenyl Cyanomethyl 441 441 493 Isopropyl Phenyl 2,4-Pentadienyl 497 497 494 Isopropyl Phenyl 4-(2,5-Cl2pyridyl)methyl 576 576 32 WO 2004/108731 PCT/US2003/017188 NO. R a R b c Mol. M+H Weight 495 Isopropyl Phenyl Chromen-2-one-3-methyl 575 575 496 Isopropyl Phenyl Methoxymethyl 475 475 497 Isopropyl Phenyl Pyran-2-one-5-methyl 525 525 498 Isopropyl Phenyl Ethyl 459 459 499 Isopropyl Phenyl 2-Ethyldecanyl 529 529 500 Isopropyl Phenyl Pyrazine-2-methyl 509 509 501 Isopropyl Phenyl 4-Pyridylmethyl 508 508 502 Isopropyl Phenyl 1-Butenyl 485 485 503 Isopropyl Phenyl 2-Nitro-5-Chlorophenyl 586 586 504 Isopropyl Phenyl Cyanomethyl 470 470 505 Isobutyl Phenyl 2,4-Pentadienyl 511 511 506 Isobutyl Phenyl 4-(2,5-Cl2pyridyl)methyl 590 590 507 Isobutyl Phenyl Chromen-2-one-3-methyl 589 589 508 Isobutyl Phenyl Methoxymethyl 489 489 509 Isobutyl Phenyl Pyran-2-one-5-methyl 539 539 510 Isobutyl Phenyl Ethyl 473 473 511 Isobutyl Phenyl 2-Ethyldecanyl 543 543 512 Isobutyl Phenyl Pyrazine-2-methyl 523 523 513 Isobutyl Phenyl 4-Pyridylmethyl 522 522 514 Isobutyl Phenyl 1-Butenyl 499 499 515 Isobutyl Phenyl 2-Nitro-5-Chlorophenyl 600 600 516 Isobutyl Phenyl Cyanomethyl 484 484 517 Benzyl Phenyl 2,4-Pentadienyl 545 545 518 Benzyl Phenyl 4-(2,5-Cl2pyridyl)methyl 624 624 519 Benzyl Phenyl Chromen-2-one-3-methyl 623 623 520 Benzyl Phenyl Methoxymethyl 523 523 521 Benzyl Phenyl Pyran-2-one-5-methyl 573 573 522 Benzyl Phenyl Ethyl 507 507 523 Benzyl Phenyl 2-Ethyldecanyl 577 577 524 Benzyl Phenyl Pyrazine-2-methyl 557 557 525 Benzyl Phenyl 4-Pyridylmethyl 556 556 526 Benzyl Phenyl 1-Butenyl 533 533 527 Benzyl Phenyl 2-Nitro-5-Chlorophenyl 634 634 528 Benzyl Phenyl Cyanomethyl 518 518 529 2-Methylpropyl Phenyl 2,4-Pentadienyl 511 511 530 2-Methylpropyl Phenyl 4-(2,5-Cl2pyridyl)methyl 590 590 531 2-Methylpropyl Phenyl Chromen-2-one-3-methyl 589 589 532 2-Methylpropyl Phenyl Methoxymethyl 489 489 533 2-Methylpropyl Phenyl Pyran-2-one-5-methyl 539 539 33 WO 2004/108731 PCT/US2003/017188 No. Ra R b Rc Mol. M+H Weight 534 2-Methylpropyl Phenyl Ethyl 473 473 535 2-Methylpropyl Phenyl 2-Ethyldecanyl 543 543 536 2-Methylpropyl Phenyl Pyrazine-2-methyl 523 523 537 2-Methylpropyl Phenyl 4-Pyridylmethyl 522 522 538 2-Methylpropyl Phenyl 1-Butenyl 499 499 539 2-Methylpropyl Phenyl 2-Nitro-5-Chlorophenyl 600 600 540 2-Methylpropyl Phenyl Cyanomethyl 484 484 541 Methylthioethyl Phenyl 2,4-Pentadienyl 529 529 542 Methylthioethyl Phenyl 4-(2,5-Cl2pyridyl)methyl 609 609 543 Methylthioethyl Phenyl Chromen-2-one-3-methyl 607 607 544 Methylthioethyl Phenyl Methoxymethyl 507 507 545 Methylthioethyl Phenyl Pyran-2-one-5-methyl 557 557 546 Methylthioethyl Phenyl Ethyl 491 491 547 Methylthioethyl Phenyl 2-Ethyldecanyl 561 561 548 Methylthioethyl Phenyl Pyrazine-2-methyl 541 541 549 Methylthioethyl Phenyl 4-Pyridylmethyl 540 540 550 Methylthioethyl Phenyl 1-Butenyl 517 517 551 Methylthioethyl Phenyl 2-Nitro-5-Chlorophenyl 618 618 552 Methylthioethyl Phenyl Cyanomethyl 502 502 553 4-Hydroxybenzyl Phenyl 2,4-Pentadienyl 561 561 554 4-Hydroxybenzyl Phenyl 4-(2,5-Cl2pyridyl)methyl 640 640 555 4-Hydroxybenzyl Phenyl Chromen-2-one-3-methyl 639 639 556 4-Hydroxybenzyl Phenyl Methoxymethyl 539 539 557 4-Hydroxybenzyl Phenyl Pyran-2-one-5-methyl 589 589 558 4-Hydroxybenzyl Phenyl Ethyl 523 523 559 4-Hydroxybenzyl Phenyl 2-Ethyldecanyl 593 593 560 4-Hydroxybenzyl Phenyl Pyrazine-2-methyl 573 573 561 4-Hydroxybenzyl Phenyl 4-Pyridylmethyl 572 572 562 4-Hydroxybenzyl Phenyl 1-Butenyl 549 549 563 4-Hydroxybenzyl Phenyl 2-Nitro-5-Chlorophenyl 650 650 564 4-Hydroxybenzyl Phenyl Cyanomethyl 534 534 565 2-Hydroxyethyl Phenyl 2,4-Pentadienyl 499 499 566 2-Hydroxyethyl Phenyl 4-(2,5-Cl2pyridyl)methyl 578 578 567 2-Hydroxyethyl Phenyl Chromen-2-one-3-methyl 577 577 568 2-Hydroxyethyl Phenyl Methoxymethyl 476 476 569 2-Hydroxyethyl Phenyl Pyran-2-one-5-methyl 527 527 570 2-Hydroxyethyl Phenyl Ethyl 460 460 571 2-Hydroxyethyl Phenyl 2-Ethyldecanyl 531 531 572 2-Hydroxyethyl Phenyl Pyrazine-2-methyl 511 511 34 WO 2004/108731 PCT/US2003/017188 No. R a R b Rc Mol. M+H Weight 573 2-Hydroxyethyl Phenyl 4-Pyridylmethyl 510 510 574 2-Hydroxyethyl Phenyl 1-Butenyl 487 487 575 2-Hydroxyethyl Phenyl 2-Nitro-5-Chlorophenyl 588 588 576 2-Hydroxyethyl Phenyl Cyanomethyl 471 471 577 Methyl Methyl 2,4-Pentadienyl 406 406 578 Methyl Methyl 4-(2,5-Cl2pyridyl)methyl 486 486 579 Methyl Methyl Chromen-2-one-3-methyl 484 484 580 Methyl Methyl Methoxymethyl 384 384 581 Methyl Methyl Pyran-2-one-5-methyl 434 434 582 Methyl Methyl Ethyl 368 388 583 Methyl Methyl 2-Ethyldecanyl 438 438 584 Methyl Methyl Pyrazine-2-methyl 418 418 585 Methyl Methyl 4-Pyridylmethyl 417 417 586 Methyl Methyl 1-Butenyl 394 394 587 Methyl Methyl 2-Nitro-5-Chlorophenyl 495 495 588 Methyl Methyl Cyanomethyl 434 434 589 Isopropyl Methyl 2,4-Pentadienyl 434 434 590 Isopropyl Methyl 4-(2,5-Cl2pyridyl)methyl 514 514 591 Isopropyl Methyl Chromen-2-one-3-methyl 512 512 592 Isopropyl Methyl Methoxymethyl 412 412 593 Isopropyl Methyl Pyran-2-one-5-methyl 462 462 594 Isopropyl Methyl Ethyl 396 396 595 Isopropyl Methyl 2-Ethyldecanyl 466 466 596 Isopropyl Methyl Pyrazine-2-methyl 446 446 597 Isopropyl Methyl 4-Pyridylmethyl 445 445 598 Isopropyl Methyl 1-Butenyl 422 422 599 Isopropyl Methyl 2-Nitro-5-Chlorophenyl 523 523 600 Isopropyl Methyl Cyanomethyl 462 462 601 Isobutyl Methyl 2,4-Pentadienyl 448 448 602 Isobutyl Methyl 4-(2,5-CI2pyridyl)methyl 528 528 603 Isobutyl Methyl Chromen-2-one-3-methyl 526 526 604 Isobutyl Methyl Methoxymethyl 426 426 605 Isobutyl Methyl Pyran-2-one-5-methyl 476 476 606 Isobutyl Methyl Ethyl 410 410 607 Isobutyl Methyl 2-Ethyldecanyl 480 480 608 Isobutyl Methyl Pyrazine-2-methyl 460 460 609 Isobutyl Methyl 4-Pyridylmethyl 459 459 610 Isobutyl Methyl 1-Butenyl 436 436 611 Isobutyl Methyl 2-Nitro-5-Chlorophenyl 537 537 35 WO 2004/108731 PCT/US2003/017188 No. Ra R b R c Mol. M+H Weight 612 Isobutyl Methyl Cyanomethyl 476 476 613 Benzyl Methyl 2,4-Pentadienyl 482 482 614 Benzyl Methyl 4-(2,5-CI2pyridyl)methyl 562 562 615 Benzyl Methyl Chromen-2-one-3-methyl 560 560 616 Benzyl Methyl Methoxymethyl 460 460 617 Benzyl Methyl Pyran-2-one-5-methyl 510 510 618 Benzyl Methyl Ethyl 444 444 619 Benzyl Methyl 2-Ethyldecanyl 514 514 620 Benzyl Methyl Pyrazine-2-methyl 494 494 621 Benzyl Methyl 4-Pyridylmethyl 493 493 622 Benzyl Methyl 1-Butenyl 470 470 623 Benzyl Methyl 2-Nitro-5-Chlorophenyl 571 571 624 Benzyl Methyl Cyanomethyl 510 510 625 2-Methylpropyl Methyl 2,4-Pentadienyl 448 448 626 2-Methylpropyl Methyl 4-(2,5-Cl2pyridyl)methyl 528 528 627 2-Methylpropyl Methyl Chromen-2-one-3-methyl 526 526 628 2-Methylpropyl Methyl Methoxymethyl 426 426 629 2-Methylpropyl Methyl Pyran-2-one-5-methyl 476 476 630 2-Methylpropyl Methyl Ethyl 410 410 631 2-Methylpropyl Methyl 2-Ethyldecanyl 480 480 632 2-Methylpropyl Methyl Pyrazine-2-methyl 460 460 633 2-Methylpropyl Methyl 4-Pyridylmethyl 459 459 634 2-Methylpropyl Methyl 1-Butenyl 436 436 635 2-Methylpropyl Methyl 2-Nitro-5-Chlorophenyl 537 537 636 2-Methylpropyl Methyl Cyanomethyl 476 476 637 Methylthioethyl Methyl 2,4-Pentadienyl 466 466 638 Methylthioethyl Methyl 4-(2,5-CI2pyridyl)methyl 546 546 639 Methylthioethyl Methyl Chromen-2-one-3-methyl 544 544 640 Methylthioethyl Methyl Methoxymethyl 444 444 641 Methylthioethyl Methyl Pyran-2-one-5-methyl 494 494 642 Methylthioethyl Methyl Ethyl 428 428 643 Methylthioethyl Methyl 2-Ethyldecanyl 498 498 644 Methylthioethyl Methyl Pyrazine-2-methyl 478 478 645 Methylthioethyl Methyl 4-Pyridylmethyl 477 477 646 Methylthioethyl Methyl 1-Butenyl 454 454 647 Methylthioethyl Methyl 2-Nitro-5-Chlorophenyl 555 555 648 Methylthioethyl Methyl Cyanomethyl 494 494 649 4-Hydroxybenzyl Methyl 2,4-Pentadienyl 498 498 650 4-Hydroxybenzyl Methyl 4-(2,5-Cl2pyridyl)methyl 578 578 36 WO 2004/108731 PCT/US2003/017188 No. Ra R b R c Mol. M+H Weight 651 4-Hydroxybenzyl Methyl Chromen-2-one-3-methyl 576 576 652 4-Hydroxybenzyl Methyl Methoxymethyl 476 476 653 4-Hydroxybenzyl Methyl Pyran-2-one-5-methyl 526 526 654 4-Hydroxybenzyl Methyl Ethyl 460 460 655 4-Hydroxybenzyl Methyl 2-Ethyldecanyl 530 530 656 4-Hydroxybenzyl Methyl Pyrazine-2-methyl 510 510 657 4-Hydroxybenzyl Methyl 4-Pyridylmethyl 509 509 658 4-Hydroxybenzyl Methyl 1-Butenyl 486 486 659 4-Hydroxybenzyl Methyl 2-Nitro-5-Chlorophenyl 587 587 660 4-Hydroxybenzyl Methyl Cyanomethyl 526 526 661 2-Hydroxyethyl Methyl 2,4-Pentadienyl 436 436 662 2-Hydroxyethyl Methyl 4-(2,5-Cl2pyridyl)methyl 516 516 663 2-Hydroxyethyl Methyl Chromen-2-one-3-methyl 514 514 664 2-Hydroxyethyl Methyl Methoxymethyl 414 414 665 2-Hydroxyethyl Methyl Pyran-2-one-5-methyl 464 464 666 2-Hydroxyethyl Methyl Ethyl 398 398 667 2-Hydroxyethyl Methyl 2-Ethyldecanyl 468 468 668 2-Hydroxyethyl Methyl Pyrazine-2-methyl 448 448 669 2-Hydroxyethyl Methyl 4-Pyridylmethyl 447 447 670 2-Hydroxyethyl Methyl 1-Butenyl 424 424 671 2-Hydroxyethyl Methyl 2-Nitro-5-Chlorophenyl 525 525 672 2-Hydroxyethyl Methyl Cyanomethyl 464 464 673 Cyclohexylmethyl Methoxy 4-Methoxybenzyl 559 559 674 4-Phenylbenzyl Methoxy 4-Methoxybenzyl 629 629 675 4-NO 2 -benzyl Methoxy 4-Methoxybenzyl 598 598 676 3,4-Cl 2 -benzyl Methoxy 4-Methoxybenzyl 621 621 677 Cyclopentyl(spiro) Methoxy 4-Methoxybenzyl 531 531 678 4-Methylbenzyl Methoxy 4-Methoxybenzyl 567 567 679 1-Naphthylmethyl Methoxy 4-Methoxybenzyl 603 603 680 4-F-benzyl Methoxy 4-Methoxybenzyl 571 571 681 3,4-F 2 -Benzyl Methoxy 4-Methoxybenzyl 589 589 682 Cyclohexyl Methoxy 4-Methoxybenzyl 545 545 683 2-CI-benzyl Methoxy 4-Methoxybenzyl 587 587 684 4-CI-benzyl Methoxy 4-Methoxybenzyl 587 587 685 Cyclohexylmethyl Methoxy 3,4-CI2-phenyl 583 583 686 4-Phenylbenzyl Methoxy 3,4-CI 2 -phenyl 654 654 687 4-NO 2 -benzyl Methoxy 3,4-CI 2 -phenyl 622 622 688 3,4-CI 2 -benzyl Methoxy 3,4-CI 2 -phenyl 646 646 689 Cyclopentyl(spiro) Methoxy 3,4-CI 2 -phenyl 555 555 37 WO 2004/108731 PCT/US2003/017188 No. R a R b Rc Mol. M+H Weight 690 4-Methylbenzyl Methoxy 3,4-Cf 2 -phenyl 591 591 691 1-Naphthylmethyl Methoxy 3,4-CI2-phenyl 627 627 692 4-F-benzyl Methoxy 3,4-Cl 2 -phenyl 595 595 693 3,4-F 2 -Benzyl Methoxy 3,4-CI 2 -phenyl 613 613 694 Cyclohexyl Methoxy 3,4-CI 2 -phenyl 569 569 695 2-CI-benzyl Methoxy 3,4-CI 2 -phenyl 612 612 696 4-CI-benzyl Methoxy 3,4-CI 2 -phenyl 612 612 697 Cyclohexylmethyl Methoxy 1-Naphthyl 565 565 698 4-Phenylbenzyl Methoxy 1-Naphthyl 635 835 699 4-NO 2 -benzyl Methoxy 1-Naphthyl 604 604 700 3,4-Cl 2 -benzyl Methoxy 1-Naphthyl 627 627 701 Cyclopentyl(spiro) Methoxy 1-Naphthyl 537 537 702 4-Methylbenzyl Methoxy 1-Naphthyl 573 573 703 1-Naphthylmethyl Methoxy 1-Naphthyl 609 609 704 4-F-benzyl Methoxy 1-Naphthyl 577 577 705 3,4-F 2 -Benzyl Methoxy 1-Naphthyl 595 595 706 Cyclohexyl Methoxy 1-Naphthyl 551 551 707 2-CI-benzyl Methoxy 1-Naphthyl 593 593 708 4-Cl-benzyl Methoxy 1-Naphthyl 593 593 709 Cyclohexylmethyl Methoxy Piperonyl 559 559 710 4-Phenylbenzyl Methoxy Piperonyl 629 629 711 4-NO2-benzyl Methoxy Piperonyl 598 598 712 3,4-CI 2 -benzyl Methoxy Piperonyl 621 621 713 Cyclopentyl(spiro) Methoxy Piperonyl 531 531 714 4-Methylbenzyl Methoxy Piperonyl 567 567 715 1-Naphthylmethyl Methoxy Piperonyl 603 603 716 4-F-benzyl Methoxy Piperonyl 571 571 717 3,4-F 2 -Benzyl Methoxy Piperonyl 589 589 718 Cyclohexyl Methoxy Piperonyl 545 545 719 2-CI-benzyl Methoxy Piperonyl 587 587 720 4-CI-benzyl Methoxy Piperonyl 587 587 721 Cyclohexylmethyl Methoxy 2,4,5-Trimethoxyphenyl 605 605 722 4-Phenylbenzyl Methoxy 2,4,5-Trimethoxyphenyl 675 675 723 4-NOz-benzyl Methoxy 2,4,5-Trimethoxyphenyl 644 644 724 3,4-CI 2 -benzyl Methoxy 2,4,5-Trimethoxyphenyl 668 668 725 Cyclopentyl(spiro) Methoxy 2,4,5-Trimethoxyphenyl 577 577 726 4-Methylbenzyl Methoxy 2,4,5-Trimethoxyphenyl 613 613 727 1-Naphthylmethyl Methoxy 2,4,5-Trimethoxyphenyl 649 649 728 4-F-benzyl Methoxy 2,4,5-Trimethoxyphenyl 617 617 38 WO 2004/108731 PCT/US2003/017188 No. Ra R b c Mol. M+H Weight 729 3,4-F 2 -Benzyl Methoxy 2,4,5-Trimethoxyphenyl 635 635 730 Cyclohexyl Methoxy 2,4,5-Trimethoxyphenyl 591 591 731 2-Cl-benzyl Methoxy 2,4,5-Trimethoxyphenyl 633 633 732 4-Cl-benzyl Methoxy 2,4,5-Trimethoxyphenyl 633 633 733 Cyclohexylmethyl Methoxy 3-Hydroxybenzyl 545 545 734 4-Phenylbenzyl Methoxy 3-Hydroxybenzyl 615 615 735 4-NO 2 -benzyl Methoxy 3-Hydroxybenzyl 584 584 736 3,4-CI 2 -benzyl Methoxy 3-Hydroxybenzyl 607 607 737 Cyclopentyl(spiro) Methoxy 3-Hydroxybenzyl 517 517 738 4-Methylbenzyl Methoxy 3-Hydroxybenzyl 553 553 739 1-Naphthylmethyl Methoxy 3-Hydroxybenzyl 589 589 740 4-F-benzyl Methoxy 3-Hydroxybenzyl 557 557 741 3,4-F 2 -Benzyl Methoxy 3-Hydroxybenzyl 575 575 742 Cyclohexyl Methoxy 3-Hydroxybenzyl 531 531 743 2-CI-benzyl Methoxy 3-Hydroxybenzyl 573 573 744 4-CI-benzyl Methoxy 3-Hydroxybenzyl 573 573 745 Cyclohexylmethyl Methoxy 1-Naphthylmethyl 579 579 746 4-Phenylbenzyl Methoxy 1-Naphthylmethyl 649 649 747 4-NO 2 -benzyl Methoxy 1-Naphthylmethyl 618 618 748 3,4-CI 2 -benzyl Methoxy 1-Naphthylmethyl 642 642 749 Cyclopentyl(spiro) Methoxy 1-Naphthylmethyl 551 551 750 4-Methylbenzyl Methoxy 1-Naphthylmethyl 587 587 751 1-Naphthylmethyl Methoxy 1-Naphthylmethyl 623 623 752 4-F-benzyl Methoxy 1-Naphthylmethyl 591 591 753 3,4-F 2 -Benzyl Methoxy 1-Naphthylmethyl 609 609 754 Cyclohexyl Methoxy 1-Naphthylmethyl 565 565 755 2-Cl-benzyl Methoxy 1-Naphthylmethyl 607 607 756 4-CI-benzyl Methoxy 1-Naphthylmethyl 607 607 757 Cyclohexylmethyl Methoxy Phenethyl 543 543 758 4-Phenylbenzyl Methoxy Phenethyl 613 613 759 4-NO 2 -benzyl Methoxy Phenethyl 582 582 760 3,4-Cl 2 -benzyl Methoxy Phenethyl 605 605 761 Cyclopentyl(spiro) Methoxy Phenethyl 515 515 762 4-Methylbenzyl Methoxy Phenethyl 551 551 763 1-Naphthylmethyl Methoxy Phenethyl 587 587 764 4-F-benzyl Methoxy Phenethyl 555 555 765 3,4-F 2 -Benzyl Methoxy Phenethyl 573 573 766 Cyclohexyl Methoxy Phenethyl 529 529 767 2-Cl-benzyl Methoxy Phenethyl 571 571 39 WO 2004/108731 PCT/US2003/017188 No. R a R b Rc Mol. M+H Weight 768 4-CI-benzyl Methoxy Phenethyl 571 571 769 Cyclohexylmethyl Methoxy 3-Methoxyphenyl 545 545 770 4-Phenylbenzyl Methoxy 3-Methoxyphenyl 615 615 771 4-NO 2 -benzyl Methoxy 3-Methoxyphenyl 584 584 772 3,4-CI2-benzyl Methoxy 3-Methoxyphenyl 607 607 773 Cyclopentyl(spiro) Methoxy 3-Methoxyphenyl 517 517 774 4-Methylbenzyl Methoxy 3-Methoxyphenyl 553 553 775 1-Naphthylmethyl Methoxy 3-Methoxyphenyl 589 589 776 4-F-benzyl Methoxy 3-Methoxyphenyl 557 557 777 3,4-F 2 -Benzyl Methoxy 3-Methoxyphenyl 575 575 778 Cyclohexyl Methoxy 3-Methoxyphenyl 531 531 779 2-Cl-benzyl Methoxy 3-Methoxyphenyl 573 573 780 4-CI-benzyl Methoxy 3-Methoxyphenyl 573 573 781 Cyclohexylmethyl Methoxy N-Benzoylaminoethyl 572 572 782 4-Phenylbenzyl Methoxy N-Benzoylaminoethyl 642 642 783 4-NO 2 -benzyl Methoxy N-Benzoylaminoethyl 611 611 784 3,4-CI 2 -benzyl Methoxy N-Benzoylaminoethyl 634 634 785 Cyclopentyl(spiro) Methoxy N-Benzoylaminoethyl 544 544 786 4-Methylbenzyl Methoxy N-Benzoylaminoethyl 580 580 787 1-Naphthylmethyl Methoxy N-Benzoylaminoethyl 616 616 788 4-F-benzyl Methoxy N-Benzoylaminoethyl 584 584 789 3,4-F 2 -Benzyl Methoxy N-Benzoylaminoethyl 602 602 790 Cyclohexyl Methoxy N-Benzoylaminoethyl 558 558 791 2-Cl-benzyl Methoxy N-Benzoylaminoethyl 600 600 792 4-CI-benzyl Methoxy N-Benzoylaminoethyl 600 600 793 Cyclohexylmethyl Methoxy Benzyl 529 529 794 4-Phenylbenzyl Methoxy Benzyl 599 599 795 4-NO 2 -benzyl Methoxy Benzyl 568 568 796 3,4-CI 2 -benzyl Methoxy Benzyl 591 591 797 Cyclopentyl(spiro) Methoxy Benzyl 501 501 798 4-Methylbenzyl Methoxy Benzyl 537 537 799 1-Naphthylmethyl Methoxy Benzyl 573 573 800 4-F-benzyl Methoxy Benzyl 541 541 801 3,4-F 2 -Benzyl Methoxy Benzyl 559 559 802 Cyclohexyl Methoxy Benzyl 515 515 803 2-CI-benzyl Methoxy Benzyl 557 557 804 4-CI-benzyl Methoxy Benzyl 557 557 805 Cyclohexylmethyl Methoxy 4-NO 2 -benzyl 574 574 806 4-Phenylbenzyl Methoxy 4-NO 2 -benzyl 644 644 40 WO 2004/108731 PCT/US2003/017188 No. Ra R Rc Mol. M+H Weight 807 4-NO 2 -benzyl Methoxy 4-NO 2 -benzyl 613 613 808 3,4-CI2-benzyl Methoxy 4-NO2-benzyl 636 636 809 Cyclopentyl(spiro) Methoxy 4-NO 2 -benzyl 546 546 810 4-Methylbenzyl Methoxy 4-NO 2 -benzyl 582 582 811 1-Naphthylmethyl Methoxy 4-NO 2 -benzyl 618 618 812 4-F-benzyl Methoxy 4-NO 2 -benzyl 586 586 813 3,4-F 2 -Benzyl Methoxy 4-NO 2 -benzyl 604 604 814 Cyclohexyl Methoxy 4-NO2-benzyl 560 560 815 2-Cl-benzyl Methoxy 4-NO2-benzyl 602 602 816 4-CI-benzyl Methoxy 4-NO2-benzyl 602 602 817 Cyclohexylmethyl Methoxy 2,4-Pentadienyl 505 505 818 4-Phenylbenzyl Methoxy 2,4-Pentadienyl 575 575 819 4-NO 2 -benzyl Methoxy 2,4-Pentadienyl 544 544 820 3,4-Cl 2 -benzyl Methoxy 2,4-Pentadienyl 567 567 821 Cyclopentyl(spiro) Methoxy 2,4-Pentadienyl 477 477 822 4-Methylbenzyl Methoxy 2,4-Pentadienyl 513 513 823 1-Naphthylmethyl Methoxy 2,4-Pentadienyl 549 549 824 4-F-benzyl Methoxy 2,4-Pentadienyl 517 517 825 3,4-F 2 -Benzyl Methoxy 2,4-Pentadienyl 535 535 826 Cyclohexyl Methoxy 2,4-Pentadienyl 491 491 827 2-Cl-benzyl Methoxy 2,4-Pentadienyl 533 533 828 4-CI-benzyl Methoxy 2,4-Pentadienyl 533 533 829 Cyclohexylmethyl Methoxy 3-(2,6-CI 2 -pyridyl)methyl 584 584 830 4-Phenylbenzyl Methoxy 3-(2,6-Cl 2 -pyridyl)methyl 655 655 831 4-NO 2 -benzyl Methoxy 3-(2,6-Cl 2 -pyridyl)methyl 623 623 832 3,4-CI 2 -benzyl Methoxy 3-(2,6-Cl 2 -pyridyl)methyl 647 647 833 Cyclopentyl(spiro) Methoxy 3-(2,6-Cl 2 -pyridyl)methyl 556 556 834 4-Methylbenzyl Methoxy 3-(2,6-Cl 2 -pyridyl)methyl 592 592 835 1-Naphthylmethyl Methoxy 3-(2,6-Cl 2 -pyridyl)methyl 628 628 836 4-F-benzyl Methoxy 3-(2,6-Cl 2 -pyridyl)methyl 596 596 837 3,4-F 2 -Benzyl Methoxy 3-(2,6-Cl 2 -pyridyl)methyl 614 614 838 Cyclohexyl Methoxy 3-(2,6-Cl2-pyridyl)methyl 570 570 839 2-CI-benzyl Methoxy 3-(2,6-Cl 2 -pyridyl)methyl 613 613 840 4-CI-benzyl Methoxy 3-(2,6-Cl 2 -pyridyl)methyl 613 613 841 Cyclohexylmethyl Methoxy Chromen-2-one-3-methyl 583 583 842 4-Phenylbenzyl Methoxy Chromen-2-one-3-methyl 653 653 843 4-NO 2 -benzyl Methoxy Chromen-2-one-3-methyl 622 622 844 3,4-CI2-benzyl Methoxy Chromen-2-one-3-methyl 645 645 845 Cyclopentyl(spiro) Methoxy Chromen-2-one-3-methyl 555 555 41 WO 2004/108731 PCT/US2003/017188 No. R a R b Rc Mof. M+H Weight 846 4-Methylbenzyl Methoxy Chromen-2-one-3-methyl 591 591 847 1-Naphthylmethyl Methoxy Chromen-2-one-3-methyl 627 627 848 4-F-benzyl Methoxy Chromen-2-one-3-methyl 595 595 849 3,4-F 2 -Benzyl Methoxy Chromen-2-one-3-methyl 613 613 850 Cyclohexyl Methoxy Chromen-2-one-3-methyl 569 569 851 2-CI-benzyl Methoxy Chromen-2-one-3-methyl 611 611 852 4-CI-benzyl Methoxy Chromen-2-one-3-methyl 611 611 853 Cyclohexylmethyl Methoxy Methoxymethyl 483 483 854 4-Phenylbenzyl Methoxy Methoxymethyl 553 553 855 4-NO 2 -benzyl Methoxy Methoxymethyl 521 521 856 3,4-CI 2 -benzyl Methoxy Methoxymethyl 545 545 857 Cyclopentyl(spiro) Methoxy Methoxymethyl 454 454 858 4-Methylbenzyl Methoxy Methoxymethyl 491 491 859 1-Naphthylmethyl Methoxy Methoxymethyl 527 527 860 4-F-benzyl Methoxy Methoxymethyl 494 494 861 3,4-F 2 -Benzyl Methoxy Methoxymethyl 512 512 862 Cyclohexyl Methoxy Methoxymethyl 469 469 863 2-Cl-benzyl Methoxy Methoxymethyl 511 511 864 4-CI-benzyl Methoxy Methoxymethyl 511 511 The synthesis of peptide mimetics in a library of the present invention may be accomplished using the general scheme of P-strand mimetics 5 library as shown in Figure 2. The synthesis of selected peptide mimetics of a bicyclic template library of the present invention was accomplished using a FlexChem Reactor Block which has a 96 well plate. In the above scheme 'Pol' represents 2-chlorotrityl chloride resin (Novabiochem) and a detailed procedure is provided below. 10 Step I The 2-chlorotrityl chloride resin (lmmol/g) and a solution Fmoc-Rl-beta-Amino Acid (1.5 equiv.) and DIEA (2 equiv.) in DCE were placed in a 96 well Robinson block (Flexchem). The reaction mixture was shaken for 12 hours at room temperature. The resin was washed with DMF, MeOH, and then DCM. 15 Step2 To the resin swollen by DMF before reaction was added 25% piperidine in DMF. Thereafter, the reaction mixture was shaken for 30 min at room temperature. The deprotection step was repeated and then the product mixture was washed with DMF, MeOH, and then DCM. A solution of 4-R 2 -amino-2-Fmoc-aminobutyric acid (1.5 equiv.), DIC (1.5 equiv.), 20 HOBT (1.5 equiv.) in NMP was added to the resin. After the reaction mixture 42 WO 2004/108731 PCT/US2003/017188 was shaken for 12 hours at room temperature, the resin was washed with DMF, MeOH, and then DCM. Step 3 To the resin swollen by DMF before reaction was added 25% piperidine in DMF. Thereafter, the reaction mixture was shaken 5 for 30 min at room temperature. The deprotection step was repeated and the product mixture was washed with DMF, MeOH, and then DCM. A solution of 2-(9H-fluoren-9-ylmethoxycarbonylamino)-5,5-dimethoxy-pentanoic acid (1.5 equiv.), DIC (1.5 equiv.), and HOBT (1.5 equiv.) in NMP was added to the resin. After the reaction mixture was shaken for 12 hours at room 10 temperature, the resin was washed with DMF, MeOH, and then DCM. Step 4 To the resin swollen by DMF before reaction was added 25% piperidine in DMF. Thereafter, the reaction mixture was shaken for 30 min at room temperature. The deprotection step was repeated and then the product mixture was washed with DMF, MeOH, and then DCM. A 15 solution of commercially available R 3 -acid (1.5 equiv.), DIC (1.5 equiv.), and HOBT (1.5 equiv.) in NMP was added to the resin. After the reaction mixture was shaken for 12 hours at room temperature, the resin was washed with DMF, MeOH, and then DCM. Step 5 The resin was treated with formic acid (1.2 mL each 20 well) for 18 hours at room temperature. Thereafter, the resin was removed by filtration, and the filtrate was condensed under reduced pressure using SpeedVac (Servant) to give the product as oil. These products were diluted with 50% water/acetonitrile and then lyophilized after freezing. Table 3 shows a P-strand mimetics library which can be prepared 25 according to the present invention, of which representative preparation is given in Example 10. Compounds of Table 3 illustrate one aspect of the invention, namely compounds wherein A is -(CH)-, B is -(CH 2 ) m- with m = 1, W is nothing, i.e., it is a direct bond between Rb and N of the heterocyclic ring, X is -NH(C=O)-, Y is oxygen, Z is hydrogen so that C=Z represents CH 2 , L is 30 -C(=O)NHR 3 , n=0, R 4 is hydrogen, and R 1 , R 2 , and R 3 are the same or different and are independently selected from an amino acid side chain moiety or derivative thereof, the remainder of the molecule, a linker and a solid support, and stereoisomers thereof. In various embodiments of this aspect of the invention, R 1 , R 2 , and R 3 are independently selected from relatively low 35 molecular weight moieties, i.e., organic groups having molecular weights of between 15 (methyl) and 1,000 g/mol; and/or at least one of R 1 , R 2 , and R 3 43 WO 2004/108731 PCT/US2003/017188 represents an amino acid side chain or derivative thereof. For example, in the compounds of Table 3, R 3 represents glutaric acid derivatives. In one aspect, the compounds of the present invention have a molecular weight within the range of about 450 to 800 g/mol, where the compounds of Table 3 provide 5 numerous illustrations of such compounds. 44 WO 2004/108731 PCT/US2003/017188 TABLE 3 THE BETA-STRAND MIMETICS LIBRARY Rb I N NN 0 0 O OH 5 H Ra 5 No. R a R RO Mol. M+H Weight 865 Propyl Phenyl 4-Methoxybenzyl 565 565 866 Propyl Phenyl 3,4-CI 2 -benzyl 585 585 867 Propyl Phenyl 1-Naphthyl 589 589 868 Propyl Phenyl Piperonyl 549 549 869 Propyl Phenyl 2,4,5-Trimethoxyphenyl 571 571 870 Propyl Phenyl 3-Hydroxybenzyl 551 551 871 Propyl Phenyl 1-Naphthylmethyl 565 565 872 Propyl Phenyl Phenethyl 578 578 873 Propyl Phenyl 3-Methoxyphenyl 611 611 874 Propyl Phenyl N-Benzoylaminoethyl 535 535 875 Propyl Phenyl Benzyl 551 551 876 Propyl Phenyl 4-NO 2 -benzyl 580 580 877 Propyl Methoxy 4-Methoxybenzyl 519 519 878 Propyl Methoxy 3,4-Cl 2 -benzyl 539 539 879 Propyl Methoxy 1-Naphthyl 543 543 880 Propyl Methoxy Piperonyl 503 503 881 Propyl Methoxy 2,4,5-Trimethoxyphenyl 525 525 882 Propyl Methoxy 3-Hydroxybenzyl 505 505 883 Propyl Methoxy 1-Naphthylmethyl 519 519 884 Propyl Methoxy Phenethyl 532 532 885 Propyl Methoxy 3-Methoxyphenyl 565 565 886 Propyl Methoxy N-Benzoylaminoethyl 489 489 887 Propyl Methoxy Benzyl 505 505 888 Propyl Methoxy 4-NO2-benzyl 534 534 889 Isobutyl Phenyl 4-Methoxybenzyl 593 593 890 Isobutyl Phenyl 3,4-Cl2-benzyl 613 613 45 WO 2004/108731 PCT/US2003/017188 No. Ra R b Rc Mol. M+H Weight 891 Isobutyl Phenyl 1-Naphthyl 618 618 892 Isobutyl Phenyl Piperonyl 577 577 893 Isobutyl Phenyl 2,4,5-Trimethoxyphenyl 599 599 894 Isobutyl Phenyl 3-Hydroxybenzyl 579 579 895 Isobutyl Phenyl 1-Naphthylmethyl 593 593 896 Isobutyl Phenyl Phenethyl 606 606 897 Isobutyl Phenyl 3-Methoxyphenyl 639 639 898 Isobutyl Phenyl N-Benzoylaminoethyl 563 563 899 Isobutyl Phenyl Benzyl 579 579 900 Isobutyl Phenyl 4-NO 2 -benzyl 608 608 901 Isobutyl Methoxy 4-Methoxybenzyl 547 547 902 Isobutyl Methoxy 3,4-Cl2-benzyl 567 567 903 Isobutyl Methoxy 1-Naphthyl 571 571 904 Isobutyl Methoxy Piperonyl 531 531 905 Isobutyl Methoxy 2,4,5-Trimethoxyphenyl 553 553 906 Isobutyl Methoxy 3-Hydroxybenzyl 533 533 907 Isobutyl Methoxy 1-Naphthylmethyl 547 547 908 Isobutyl Methoxy Phenethyl 560 580 909 Isobutyl Methoxy 3-Methoxyphenyl 593 593 910 Isobutyl Methoxy N-Benzoylaminoethyl 517 517 911 Isobutyl Methoxy Benzyl 533 533 912 Isobutyl Methoxy 4-NO 2 -benzyl 562 562 913 4-Br-benzyl Phenyl 4-Methoxybenzyl 692 692 914 4-Br-benzyl Phenyl 3,4-C 2 -benzyl 712 712 915 4-Br-benzyl Phenyl 1-Naphthyl 716 716 916 4-Br-benzyl Phenyl Piperonyl 676 676 917 4-Br-benzyl Phenyl 2,4,5-Trimethoxyphenyl 698 698 918 4-Br-benzyl Phenyl 3-Hydroxybenzyl 678 678 919 4-Br-benzyl Phenyl 1-Naphthylmethyl 692 692 920 4-Br-benzyl Phenyl Phenethyl 705 705 921 4-Br-benzyl Phenyl 3-Methoxyphenyl 738 738 922 4-Br-benzyl Phenyl N-Benzoylaminoethyl 662 662 923 4-Br-benzyl Phenyl Benzyl 678 678 924 4-Br-benzyl Phenyl 4-NO 2 -benzyl 707 707 925 4-Br-benzyl Methoxy 4-Methoxybenzyl 646 646 926 4-Br-benzyl Methoxy 3,4-Cl 2 -benzyl 666 666 927 4-Br-benzyl Methoxy 1-Naphthyl 670 670 928 4-Br-benzyl Methoxy Piperonyl 630 630 929 4-Br-benzyl Methoxy 2,4,5-Trimethoxyphenyl 652 652 46 WO 2004/108731 PCT/US2003/017188 No. R a Rb R c Mol. M+H Weight 930 4-Br-benzyl Methoxy 3-Hydroxybenzyl 631 631 931 4-Br-benzyl Methoxy 1-Naphthylmethyl 645 645 932 4-Br-benzyl Methoxy Phenethyl 659 659 933 4-Br-benzyl Methoxy 3-Methoxyphenyl 692 692 934 4-Br-benzyl Methoxy N-Benzoylaminoethyl 615 615 935 4-Br-benzyl Methoxy Benzyl 631 631 936 4-Br-benzyl Methoxy 4-NO 2 -benzyl 660 660 937 Benzyl Phenyl 4-Methoxybenzyl 613 613 938 Benzyl Phenyl 3,4-Cl 2 -benzyl 633 633 939 Benzyl Phenyl 1-Naphthyl 638 638 940 Benzyl Phenyl Piperonyl 597 597 941 Benzyl Phenyl 2,4,5-Trimethoxyphenyl 619 619 942 Benzyl Phenyl 3-Hydroxybenzyl 599 599 943 Benzyl Phenyl 1-Naphthylmethyl 613 613 944 Benzyl Phenyl Phenethyl 626 626 945 Benzyl Phenyl 3-Methoxyphenyl 659 659 946 Benzyl Phenyl N-Benzoylaminoethyl 583 583 947 Benzyl Phenyl Benzyl 599 599 948 Benzyl Phenyl 4-NO 2 -benzyl 628 628 949 Benzyl Methoxy 4-Methoxybenzyl 567 567 950 Benzyl Methoxy 3,4-CI 2 -benzyl 587 587 951 Benzyl Methoxy 1-Naphthyl 591 591 952 Benzyl Methoxy Piperonyl 551 551 953 Benzyl Methoxy 2,4,5-Trimethoxyphenyl 573 573 954 Benzyl Methoxy 3-Hydroxybenzyl 553 553 955 Benzyl Methoxy 1-Naphthylmethyl 567 567 956 Benzyl Methoxy Phenethyl 580 580 957 Benzyl Methoxy 3-Methoxyphenyl 613 613 958 Benzyl Methoxy N-Benzoylaminoethyl 537 537 959 Benzyl Methoxy Benzyl 553 553 960 Benzyl Methoxy 4-NO 2 -benzyl 582 582 961 Propyl Benzyloxy 2,4-Pentadienyl 541 541 962 Propyl Benzyloxy 3-(2,6-CI 2 -pyridyl)methyl 620 620 963 Propyl Benzyloxy Chromen-2-one-3- 619 619 methyl 619__19 964 Propyl Benzyloxy Methoxymethyl 519 519 965 Propyl Benzyloxy Pyran-2-one-4-methyl 569 569 966 Propyl Benzyloxy Ethyl 503 503 967 Propyl Benzyloxy 2-Ethyldecanyl 629 629 968 Propyl Benzyloxy Pyrazin-2-methyl 553 553 47 WO 2004/108731 PCT/US2003/017188 No. Ra R R c Mol. M+H Weight 969 Propyl Benzyloxy 4-Pyridylmethyl 552 552 970 Propyl Benzyloxy 4-Butenyl 529 529 971 Propyl Benzyloxy 2-NO 2 -5-CI-phenyl 630 630 972 Propyl Benzyloxy Cyanomethyl 514 514 973 Propyl Methoxy 2,4-Pentadienyl 465 465 974 Propyl Methoxy 3-(2,6-Cl 2 -pyridyl)methyl 544 544 975 Propyl Methoxy Chromen-2-one-3- 543 543 976 Propyl Methoxy Methoxymethyl 442 442 977 Propyl Methoxy Pyran-2-one-4-methyl 492 492 978 Propyl Methoxy Ethyl 426 426 979 Propyl Methoxy 2-Ethyldecanyl 553 553 980 Propyl Methoxy Pyrazin-2-methyl 476 476 981 Propyl Methoxy 4-Pyridylmethyl 476 476 982 Propyl Methoxy 4-Butenyl 453 453 983 Propyl Methoxy 2-NO2-5-CI-phenyl 554 554 984 Propyl Methoxy Cyanomethyl 437 437 985 Isobutyl Benzyloxy 2,4-Pentadienyl 569 569 986 Isobutyl Benzyloxy 3-(2,6-CI2-pyridyl)methyl 649 649 987 Isobutyl Benzyloxy Chromen-2-one-3- 647 647 methyl 988 Isobutyl Benzyloxy Methoxymethyl 547 547 989 Isobutyl Benzyloxy Pyran-2-one-4-methyl 597 597 990 Isobutyl Benzyloxy Ethyl 531 531 991 Isobutyl Benzyloxy 2-Ethyldecanyl 657 657 992 Isobutyl Benzyloxy Pyrazin-2-methyl 581 581 993 Isobutyl Benzyloxy 4-Pyridylmethyl 580 580 994 Isobutyl Benzyloxy 4-Butenyl 557 557 995 Isobutyl Benzyloxy 2-NO 2 -5-Cl-phenyl 658 658 996 Isobutyl Benzyloxy Cyanomethyl 542 542 997 Isobutyl Methoxy 2,4-Pentadienyl 493 493 998 Isobutyl Methoxy 3-(2,6-Cl 2 -pyridyl)methyl 572 572 999 Isobutyl Methoxy Chromen-2-one-3- 571 571 1000 Isobutyl Methoxy Methoxymethyl 471 471 1001 Isobutyl Methoxy Pyran-2-one-4-methyl 521 521 1002 Isobutyl Methoxy Ethyl 455 455 1003 Isobutyl Methoxy 2-Ethyldecanyl 581 581 1004 Isobutyl Methoxy Pyrazin-2-methyl 505 505 1005 Isobutyl Methoxy 4-Pyridylmethyl 504 504 1006 Isobutyl Methoxy 4-Butenyl 481 481 1007 Isobutyl Methoxy 2-NO 2 -5-CI-phenyl 582 582 48 WO 2004/108731 PCT/US2003/017188 No. Ra R b Rc Mol. M+H Weight 1008 Isobutyl Methoxy Cyanomethyl 466 466 1009 Benzyl Benzyloxy 2,4-Pentadienyl 589 589 1010 Benzyl Benzyloxy 3-(2,6-Cl 2 -pyridyl)methyl 669 669 1011 Benzyl Benzyloxy Chromen-2-one-3- 667 667 Benzyloxy methyl 1012 Benzyl Benzyloxy Methoxymethyl 567 567 1013 Benzyl Benzyloxy Pyran-2-one-4-methyl 617 617 1014 Benzyl Benzyloxy Ethyl 551 551 1015 Benzyl Benzyloxy 2-Ethyldecanyl 677 677 1016 Benzyl Benzyloxy Pyrazin-2-methyl 601 601 1017 Benzyl Benzyloxy 4-Pyridylmethyl 600 600 1018 Benzyl Benzyloxy 4-Butenyl 577 577 1019 Benzyl Benzyloxy 2-NO 2 -5-Cl-phenyl 678 678 1020 Benzyl Benzyloxy Cyanomethyl 562 562 1021 Benzyl Methoxy 2,4-Pentadienyl 513 513 1022 Benzyl Methoxy 3-(2,6-CI 2 -pyridyl)methyl 592 592 1023 Benzyl Methoxy Chromen-2-one-3- 591 591 methyl 1024 Benzyl Methoxy Methoxymethyl 491 491 1025 Benzyl Methoxy Pyran-2-one-4-methyl 541 541 1026 Benzyl Methoxy Ethyl 475 475 1027 Benzyl Methoxy 2-Ethyldecanyl 601 601 1028 Benzyl Methoxy Pyrazin-2-methyl 525 525 1029 Benzyl Methoxy 4-Pyridylmethyl 524 524 1030 Benzyl Methoxy 4-Butenyl 501 501 1031 Benzyl Methoxy 2-NO 2 -5-Cl-phenyl 602 602 1032 Benzyl Methoxy Cyanomethyl 486 486 1033 Phenylpropyl Benzyloxy 2,4-Pentadienyl 617 617 1034 Phenylpropyl Benzyloxy 3-(2,6-Cl 2 -pyridyl)methyl 697 697 1035 Phenylpropyl Benzyloxy Chromen-2-one-3- 695 695 methyl_____ 1036 Phenylpropyl Benzyloxy Methoxymethyl 595 595 1037 Phenylpropyl Benzyloxy Pyran-2-one-4-methyl 645 645 1038 Phenylpropyl Benzyloxy Ethyl 579 579 1039 Phenylpropyl Benzyloxy 2-Ethyldecanyl 705 705 1040 Phenylpropyl Benzyloxy Pyrazin-2-methyl 629 629 1041 Phenylpropyl Benzyloxy 4-Pyridylmethyl 628 628 1042 Phenylpropyl Benzyloxy 4-Butenyl 605 605 1043 Phenylpropyl Benzyloxy 2-NO 2 -5-Cl-phenyl 706 706 1044 Phenylpropyl Benzyloxy Cyanomethyl 590 590 1045 Phenylpropyl Methoxy 2,4-Pentadienyl 541 541 1046 Phenylpropyl Methoxy 3-(2,6-Cl 2 -pyridyl)methyl 620 620 49 WO 2004/108731 PCT/US2003/017188 No. R a R Rc Mol. M+H Weight 1047 Phenylpropyl Methoxy Chromen-2-one-3- 619 619 Methyl 1048 Phenylpropyl Methoxy Methoxymethyl 519 519 1049 Phenylpropyl Methoxy Pyran-2-one-4-methyl 569 569 1050 Phenylpropyl Methoxy Ethyl 503 503 1051 Phenylpropyl Methoxy 2-Ethyldecanyl 629 629 1052 Phenylpropyl Methoxy Pyrazin-2-methyl 553 553 1053 Phenylpropyl Methoxy 4-Pyridylmethyl 552 552 1054 Phenylpropyl Methoxy 4-Butenyl 529 529 1055 Phenylpropyl Methoxy 2-NO 2 -5-Cl-phenyl 630 630 1056 Phenylpropyl Methoxy Cyanomethyl 514 514 1057 Methyl Methoxy 4-Methoxybenzyl 491 491 1058 Methyl Methoxy 3,4-CI 2 -benzyl 515 515 1059 Methyl Methoxy 1-Naphthyl 497 497 1060 Methyl Methoxy Piperonyl 490 490 1061 Methyl Methoxy 2,4,5-Trimethoxyphenyl 537 537 1062 Methyl Methoxy 3-Hydroxybenzyl 476 476 1063 Methyl Methoxy 1-Naphthylmethyl 511 511 1064 Methyl Methoxy Phenethyl 475 475 1065 Methyl Methoxy 3-Methoxyphenyl 476 476 1066 Methyl Methoxy N-Benzoylaminoethyl 504 504 1067 Methyl Methoxy Benzyl 460 460 1068 Methyl Methoxy 4-NO 2 -benzyl 505 505 1069 Amino Methoxy 4-Methoxybenzyl 492 492 1070 Amino Methoxy 3,4-CI 2 -benzyl 516 516 1071 Amino Methoxy 1-Naphthyl 498 498 1072 Amino Methoxy Piperonyl 491 491 1073 Amino Methoxy 2,4,5-Trimethoxyphenyl 538 538 1074 Amino Methoxy 3-Hydroxybenzyl 477 477 1075 Amino Methoxy 1-Naphthylmethyl 512 512 1076 Amino Methoxy Phenethyl 476 476 1077 Amino Methoxy 3-Methoxyphenyl 477 477 1078 Amino Methoxy N-Benzoylaminoethyl 505 505 1079 Amino Methoxy Benzyl 461 461 1080 Amino Methoxy 4-NO 2 -benzyl 506 506 1081 3-Propenyl Methoxy 4-Methoxybenzyl 517 517 1082 3-Propenyl Methoxy 3,4-Cl 2 -benzyl 541 541 1083 3-Propenyl Methoxy 1-Naphthyl 523 523 1084 3-Propenyl Methoxy Piperonyl 517 517 1085 3-Propenyl Methoxy 2,4,5-Trimethoxyphenyl 563 563 50 WO 2004/108731 PCT/US2003/017188 No. Ra Rb Re Mol. M+H Weight 1086 3-Propenyl Methoxy 3-Hydroxybenzyl 503 503 1087 3-Propenyl Methoxy 1-Naphthylmethyl 537 537 1088 3-Propenyl Methoxy Phenethyl 501 501 1089 3-Propenyl Methoxy 3-Methoxyphenyl 503 503 1090 3-Propenyl Methoxy N-Benzoylaminoethyl 530 530 1091 3-Propenyl Methoxy Benzyl 487 487 1092 3-Propenyl Methoxy 4-NO2-benzyl 532 532 1093 Ethanoic acid Methoxy 4-Methoxybenzyl 535 535 1094 Ethanoic acid Methoxy 3,4-CI 2 -benzyl 559 559 1095 Ethanoic acid Methoxy 1-Naphthyl 541 541 1096 Ethanoic acid Methoxy Piperonyl 534 534 1097 Ethanoic acid Methoxy 2,4,5-Trimethoxyphenyl 581 581 1098 Ethanoic acid Methoxy 3-Hydroxybenzyl 521 521 1099 Ethanoic acid Methoxy 1-Naphthylmethyl 555 555 1100 Ethanoic acid Methoxy Phenethyl 519 519 1101 Ethanoic acid Methoxy 3-Methoxyphenyl 521 521 1102 Ethanoic acid Methoxy N-Benzoylaminoethyl 548 548 1103 Ethanoic acid Methoxy Benzyl 505 505 1104 Ethanoic acid Methoxy 4-NO 2 -benzyl 549 549 1105 Propionic acid Methoxy 4-Methoxybenzyl 549 549 1106 Propionic acid Methoxy 3,4-Cl 2 -benzyl 573 573 1107 Propionic acid Methoxy 1-Naphthyl 555 555 1108 Propionic acid Methoxy Pipemronyl 549 549 1109 Propionic acid Methoxy 2,4,5-Trimethoxyphenyl 595 595 1110 Propionic acid Methoxy 3-Hydroxybenzyl 535 535 1111 Propionic acid Methoxy 1-Naphthylmethyl 569 569 1112 Propionic acid Methoxy Phenethyl 533 533 1113 Propionic acid Methoxy 3-Methoxyphenyl 535 535 1114 Propionic acid Methoxy N-Benzoylaminoethyl 562 562 1115 Propionic acid Methoxy Benzyl 519 519 1116 Propionic acid Methoxy 4-NO2-benzyl 564 564 1117 4-Vinylbenzyl Methoxy 4-Methoxybenzyl 593 593 1118 4-Vinylbenzyl Methoxy 3,4-Ci 2 -benzyl 617 617 1119 4-Vinylbenzyl Methoxy 1-Naphthyl 599 599 1120 4-Vinylbenzyl Methoxy Piperonyl 593 593 1121 4-Vinylbenzyl Methoxy 2,4,5-Trimethoxyphenyl 639 639 1122 4-Vinylbenzyl Methoxy 3-Hydroxybenzyl 579 579 1123 4-Vinylbenzyl Methoxy 1-Naphthylmethyl 613 613 1124 4-Vinylbenzyl Methoxy Phenethyl 577 577 51 WO 2004/108731 PCT/US2003/017188 No. Ra R b R Mol. M+H Weight 1125 4-Vinylbenzyl Methoxy 3-Methoxyphenyl 579 579 1126 4-Vinylbenzyl Methoxy N-Benzoylaminoethyl 606 606 1127 4-Vinylbenzyl Methoxy Benzyl 563 563 1128 4-Vinylbenzyl Methoxy 4-NO 2 -benzyl 608 608 1129 Piperonylmethyl Methoxy 4-Methoxybenzyl 611 611 1130 Piperonylmethyl Methoxy 3,4-Cl2-benzyl 635 635 1131 Piperonylmethyl Methoxy 1-Naphthyl 617 617 1132 Piperonylmethyl Methoxy Piperonyl 611 611 1133 Piperonylmethyl Methoxy 2,4,5-Trimethoxyphenyl 657 657 1134 Piperonylmethyl Methoxy 3-Hydroxybenzyl 597 597 1135 Piperonylmethyl Methoxy 1-Naphthylmethyl 631 631 1136 Piperonylmethyl Methoxy Phenethyl 595 595 1137 Piperonylmethyl Methoxy 3-Methoxyphenyl 597 597 1138 Piperonylmethyl Methoxy N-Benzoylaminoethyl 624 624 1139 Piperonylmethyl Methoxy Benzyl 581 581 1140 Piperonylmethyl Methoxy 4-NO 2 -benzyl 626 626 1141 4-F-benzyl Methoxy 4-Methoxybenzyl 585 585 1142 4-F-benzyl Methoxy 3,4-CI 2 -benzyl 609 609 1143 4-F-benzyl Methoxy 1-Naphthyl 591 591 1144 4-F-benzyl Methoxy Piperonyl 585 585 1145 4-F-benzyl Methoxy 2,4,5-Trimethoxyphenyl 631 631 1146 4-F-benzyl Methoxy 3-Hydroxybenzyl 571 571 1147 4-F-benzyl Methoxy 1-Naphthylmethyl 605 605 1148 4-F-benzyl Methoxy Phenethyl 569 569 1149 4-F-benzyl Methoxy 3-Methoxyphenyl 571 571 1150 4-F-benzyl Methoxy N-Benzoylaminoethyl 598 598 1151 4-F-benzyl Methoxy Benzyl 555 555 1152 4-F-benzyl Methoxy 4-NO 2 -benzyl 600 600 1153 Methyl Benzyloxy 4-Methoxybenzyl 567 567 1154 Methyl Benzyloxy 3,4-Cl 2 -benzyl 591 591 1155 Methyl Benzyloxy 1-Naphthyl 573 573 1156 Methyl Benzyloxy Piperonyl 567 567 1157 Methyl Benzyloxy 2,4,5-Trimethoxyphenyl 613 613 1158 Methyl Benzyloxy 3-Hydroxybenzyl 553 553 1159 Methyl Benzyloxy 1-Naphthylmethyl 587 587 1160 Methyl Benzyloxy Phenethyl 551 551 1161 Methyl Benzyloxy 3-Methoxyphenyl 553 553 1162 Methyl Benzyloxy N-Benzoylaminoethyl 580 580 1163 Methyl Benzyloxy Benzyl 537 537 52 WO 2004/108731 PCT/US2003/017188 No. Ra R b Rc Mol. M+H Weight 1164 Methyl Benzyloxy 4-NO2-benzyl 582 582 1165 Amino Benzyloxy 4-Methoxybenzyl 568 568 1166 Amino Benzyloxy 3,4-CI2-benzyl 592 592 1167 Amino Benzyloxy 1-Naphthyl 574 574 1168 Amino Benzyloxy Piperonyl 568 568 1169 Amino Benzyloxy 2,4,5-Trimethoxyphenyl 614 614 1170 Amino Benzyloxy 3-Hydroxybenzyl 554 554 1171 Amino Benzyloxy 1-Naphthylmethyl 588 588 1172 Amino Benzyloxy Phenethyl 552 552 1173 Amino Benzyloxy 3-Methoxyphenyl 554 554 1174 Amino Benzyloxy N-Benzoylaminoethyl 581 581 1175 Amino Benzyloxy Benzyl 538 538 1176 Amino Benzyloxy 4-NO 2 -benzyl 583 583 1177 3-Propenyl Benzyloxy 4-Methoxybenzyl 593 593 1178 3-Propenyl Benzyloxy 3,4-Cl 2 -benzyl 617 617 1179 3-Propenyl Benzyloxy 1-Naphthyl 599 599 1180 3-Propenyl Benzyloxy Piperonyl 593 593 1181 3-Propenyl Benzyloxy 2,4,5-Trimethoxyphenyl 639 639 1182 3-Propenyl Benzyloxy 3-Hydroxybenzyl 579 579 1183 3-Propenyl Benzyloxy 1-Naphthylmethyl 613 613 1184 3-Propenyl Benzyloxy Phenethyl 577 577 1185 3-Propenyl Benzyloxy 3-Methoxyphenyl 579 579 1186 3-Propenyl Benzyloxy N-Benzoylaminoethyl 606 606 1187 3-Propenyl Benzyloxy Benzyl 563 563 1188 3-Propenyl Benzyloxy 4-NO 2 -benzyl 608 608 1189 Ethanoic acid Benzyloxy 4-Methoxybenzyl 611 611 1190 Ethanoic acid Benzyloxy 3,4-Ci 2 -benzyl 635 635 1191 Ethanoic acid Benzyloxy 1-Naphthyl 617 617 1192 Ethanoic acid Benzyloxy Piperonyl 611 611 1193 Ethanoic acid Benzyloxy 2,4,5-Trimethoxyphenyl 657 657 1194 Ethanoic acid Benzyloxy 3-Hydroxybenzyl 597 597 1195 Ethanoic acid Benzyloxy 1-Naphthylmethyl 631 631 1196 Ethanoic acid Benzyloxy Phenethyl 595 595 1197 Ethanoic acid Benzyloxy 3-Methoxyphenyl 597 597 1198 Ethanoic acid Benzyloxy N-Benzoylaminoethyl 624 624 1199 Ethanoic acid Benzyloxy Benzyl 581 581 1200 Ethanoic acid Benzyloxy 4-NO 2 -benzyl 626 626 1201 Propionic acid Benzyloxy 4-Methoxybenzyl 625 625 1202 Propionic acid Benzyloxy 3,4-Cl2-benzyl 649 649 53 WO 2004/108731 PCT/US2003/017188 No. Ra R b Rc Mol. M+H Weight 1203 Propionic acid Benzyloxy 1-Naphthyl 631 631 1204 Propionic acid Benzyloxy Piperonyl 625 625 1205 Propionic acid Benzyloxy 2,4,5-Trimethoxyphenyl 671 671 1206 Propionic acid Benzyloxy 3-Hydroxybenzyl 611 611 1207 Propionic acid Benzyloxy 1-Naphthylmethyl 645 645 1208 Propionic acid Benzyloxy Phenethyl 609 609 1209 Propionic acid Benzyloxy 3-Methoxyphenyl 611 611 1210 Propionic acid Benzyloxy N-Benzoylaminoethyl 638 638 1211 Propionic acid Benzyloxy Benzyl 595 595 1212 Propionic acid Benzyloxy 4-NO 2 -benzyl 640 640 1213 4-Vinylbenzyl Benzyloxy 4-Methoxybenzyl 669 669 1214 4-Vinylbenzyl Benzyloxy 3,4-CI 2 -benzyl 694 694 1215 4-Vinylbenzyl Benzyloxy 1-Naphthyl 675 675 1216 4-Vinylbenzyl Benzyloxy Piperonyl 669 669 1217 4-Vinylbenzyl Benzyloxy 2,4,5-Trimethoxyphenyl 715 715 1218 4-Vinylbenzyl Benzyloxy 3-Hydroxybenzyl 655 655 1219 4-Vinylbenzyl Benzyloxy 1-Naphthylmethyl 689 689 1220 4-Vinylbenzyl Benzyloxy Phenethyl 653 653 1221 4-Vinylbenzyl Benzyloxy 3-Methoxyphenyl 655 655 1222 4-Vinylbenzyl Benzyloxy N-Benzoylaminoethyl 682 682 1223 4-Vinylbenzyl Benzyloxy Benzyl 639 639 1224 4-Vinylbenzyl Benzyloxy 4-NO 2 -benzyl 684 684 1225 Piperonylmethyl Benzyloxy 4-Methoxybenzyl 687 687 1226 Piperonylmethyl Benzyloxy 3,4-Cl 2 -benzyl 712 712 1227 Piperonylmethyl Benzyloxy 1-Naphthyl 693 693 1228 Piperonylmethyl Benzyloxy Piperonyl 687 687 1229 Piperonylmethyl Benzyloxy 2,4,5-Trimethoxyphenyl 733 733 1230 Piperonylmethyl Benzyloxy 3-Hydroxybenzyl 673 673 1231 Piperonylmethyl Benzyloxy 1-Naphthylmethyl 707 707 1232 Piperonylmethyl Benzyloxy Phenethyl 671 671 1233 Piperonylmethyl Benzyloxy 3-Methoxyphenyl 673 673 1234 Piperonylmethyl Benzyloxy N-Benzoylaminoethyl 700 700 1235 Piperonylmethyl Benzyloxy Benzyl 657 657 1236 Piperonylmethyl Benzyloxy 4-NO 2 -benzyl 702 702 1237 4-F-benzyl Benzyloxy 4-Methoxybenzyl 661 661 1238 4-F-benzyl Benzyloxy 3,4-Cl 2 -benzyl 686 686 1239 4-F-benzyl Benzyloxy 1-Naphthyl 667 667 1240 4-F-benzyl Benzyloxy Piperonyl 661 661 1241 4-F-benzyl Benzyloxy 2,4,5-Trimethoxyphenyl 707 707 54 WO 2004/108731 PCT/US2003/017188 The 1-strand mimetic structures of the present invention may be used as bioactive agents, such as diagnostic, prophylactic, and therapeutic agents. Preferably, the compounds are formulated into a pharmaceutically acceptable form and then administered to a patient in need of treatment by the 5 1-strand mimetic structures of the present invention. Thus, the present invention provides a pharmaceutical composition containing a compound of structures (1") through (1"'). For the preparation of the pharmaceutical composition containing the present compounds, a skilled person in the art can use publicly known knowledge and 10 techniques that are known in the pertinent art. Generally known varieties of carriers and other additives are used for the preparation of the composition of the present invention. The pharmaceutical compositions of this invention may be administered in a standard manner for the disease condition that is desired to be treated, for example by oral, rectal or parenteral administration. 15 For these purposes, the compounds of the present invention may be formulated by means known in the art into a form of, for example, tablets, capsules, aqueous or oily solutions or suspension, (lipid) emulsions, dispersible powders, suppositories, ointments, creams, drops and sterile injectable aqueous or oily solutions or suspensions. 20 A suitable pharmaceutical composition of the present invention is one suitable for oral administration in unit dosage form such as, for example a tablet or capsule that contains from about 1 mg to about 1 g of the compound of this invention. In another aspect, a pharmaceutical composition of the present 25 invention is one suitable for intravenous, subcutaneous or intramuscular injection. A patient may receive, for example, an intravenous, subcutaneous or intramuscular dose of about 1 pg/kg to about 1 g/kg of the compound of the present invention. The intravenous, subcutaneous and intramuscular dose may be given by means of a bolus injection. Alternatively the intravenous 30 dose may be given by continuous infusion over a period of time. Alternatively a patient will receive a daily oral dose which is. approximately equivalent to the daily parenteral dose, the composition being administered 1 to about 4 times per day. The following table illustrates representative pharmaceutical 35 dosage forms containing the compound or pharmaceutically-acceptable salt thereof for therapeutics or prophylactic use in humans: 55 WO 2004/108731 PCT/US2003/017188 Tablet 1 mg/tablet Compound 100 Lactose Ph. Eur. 179 Croscarmellose sodium 12.0 Polyvinylpyrrolidone 6 Magnesium stearate 3.0 Tablet 2 mg/tablet Compound 50 Lactose Ph. Eur. 229 Croscarmellose sodium 12.0 Polyvinylpyrrolidone 6 Magnesium stearate 3.0 Tablet 3 mg/tablet Compound 1.0 Lactose Ph. Eur. 92 Croscarmellose sodium 4.0 Polyvinylpyrrolidone 2.0 Magnesium stearate 1.0 Capsule mg/capsule Compound 10 Lactose Ph. Eur. 389 Croscarmellose sodium 100 Magnesium stearate 1.0 5 Injection I (50mg/mi) Compound 0.5% w/v Isotonic aqueous solution to 100% The pharmaceutical composition containing the compound of general formula (I) can be used for a variety of biologically desirable effects, including inhibiting a protease in a warm-blooded animal, modulating a cell 10 signaling transcription factor related peptide in a warm-blooded animal, and for inhibiting a kinase in a warm-blooded animal. These effects may be achieved by a method comprising administering to the animal in need thereof an effective amount of the compound of formula (I). Furthermore, and as discussed in detail below, the 13-strand 15 mimetic structures of the present invention may also be effective for inhibiting 56 WO 2004/108731 PCT/US2003/017188 MHC-I andlor MHC-II presentation of peptides to T cell receptors in a warm blooded animal; for inhibiting peptide binding to SH2 domains in a warm blooded animal; for inhibiting peptide binding to SH3 domains in a warm blooded animal; for inhibiting peptide binding to PTB domains in a warm 5 blooded animal; for modulating G protein coupled receptor (GPCR) and ion channel in a warm-blooded animal; and for modulating cytokines in a warm blooded animal. Kinase Inhibition (including SH2 and SH3 domain inhibition) In one aspect, the present invention provides a method for 10 inhibiting a kinase in a warm-blooded animal. The method comprises administering to the animal an amount of a compound of the present invention, where the amount is effective to inhibit a kinase. Kinases (also known as protein kinases) are a class of enzymes that catalyze a reaction whereby a biomolecule (typically another enzyme) is phosphorylated. As many as 1000 15 kinases are thought to be encoded in the mammalian genome (Hunter, Cell 50:823-829, 1987). The large number of kinases allow for rapid signal amplification and multiple points of regulation. Phosophorylation is a very common covalent modification found in signal transduction processes, and causes an alteration in the activity of 20 those proteins which become phosphorylated. Kinases are thus a critical component of signaling pathways. Kinases are typically organized into several modular functional regions, or "domains" (Cohen, G. B., et al. Cell 80:237-248, 1995). One domain, known as "SH3," is a region of 55-70 amino acids that binds to proline-rich peptides, particularly extended strand. Another 25 domain, known as "SH2," is a phosphotyrosine binding region of about 100 amino acids in length. These two domains are believed to be involved in recognizing and binding to the protein substrates. These, as well as other domains including myristoylation and palmitoylation sites, are responsible for assembling multiprotein complexes which guide the catalytic domain to the 30 correct targets (Mayer et al. MoL. Cell. Biol. 12:609-618, 1992; and Mayer and Baltimore, Mol. Cell. Biol. 14:2883-2894, 1994). While SH2 and SH3 domains are known to be present in some kinases, these domains are also present in other proteins. The compounds of the present invention may be used to inhibit SH2- or SH3-mediated binding in kinase or other proteins. 57 WO 2004/108731 PCT/US2003/017188 Kinases are used by the body in a vast number of different, but often interrelated, intracellular signal transduction mechanisms. For example, growth factors, transcription factors, hormones, cell cycle regulatory proteins, and many other classes of cellular regulators utilize tyrosine kinases in their 5 signaling cascades (see, e.g., Bolen et al. FASEB J. 6:3403-3409, 1992; and Ullrich and Schlessinger, Cell 61:203-212, 1990). The serine/threonine kinases make up the majority of the remainder of the kinase family. One important approach for determining the role, and understanding the function, of enzymes, both in vitro and in vivo, is the use of 10 specific enzyme inhibitors. If one or more compounds can be found that will inhibit the enzyme, the inhibitor can be used to modulate the enzyme's activity, and the effects of that decrease can be observed. Such approaches have been instrumental in deciphering many of the pathways of intermediary metabolism, and have also been important in learning about enzyme kinetics 15 and determining catalytic mechanisms. The present invention provides such compounds. Regulation of many immune responses is mediated through receptors that transmit signals through tyrosine kinases containing SH2 domains. T-cell activation via the antigen specific T-cell receptor (TCR) 20 initiates a signal transduction cascade leading to lymphokine secretion and cell proliferation. One of the earliest biochemical responses following TCR activation is an increase in tyrosine kinase activity. In particular, T-cell activation and proliferation is controlled through T-cell receptor mediated activation of p56ck and p59yn tyrosine kinases, as well as ZAP-70 and Syk 25 (Weiss and Litman, Cell 76:263-274,1994) which contain SH2 domains. Additional evidence indicates that several src-family kinases (Ick, blk, fyn) participate in signal transduction pathways leading from B-cell antigen receptors and hence may serve to integrate stimuli received from several independent receptor structures. Thus, inhibitors that block interactions of 30 these SH2 domain kinases with their cognate receptors could serve as immunosuppressive agents with utility in autoimmune diseases, transplant rejection or as anti-inflammatory agents as well as anticancer drugs in cases of lymphocytic leukemias. Additionally, non-transmembrane PTPase containing SH2 35 domains are known and nomenclature refers to them as SH-PTP1 and SH PTP2 (Neel, Cell Biology 4:419-432, 1993) SH-PTP1 is identical to PTP1 C, 58 WO 2004/108731 PCT/US2003/017188 HCP or SHP and SH-PTP2 is also known as PTP1D or PTP2C. SH-PTP1 is expressed at high levels in hematopoietic cells of all lineages and all stages of differentiation. Since the SH-PTP1 gene was identified as responsible for the motheaten (me) mouse phenotype, this provides a basis for predicting the 5 effects of inhibitors that would block its interaction with its cellular substrates. Thus, inhibition of SH-PTP1 function would be expected to result in impaired T cell responses to mitogenic stimulation, decreased NK cell function, and depletion of B-cell precursors with potential therapeutic applications as described above. 10 The ability of a compound of the present invention to bind to the SH2 domain of STAT6, or to bind to the SH2 domain of the protein tyrosine phosphatase SH-PTP1, can be demonstrated by the procedures disclosed by Payne et al., P.N.A.S. USA 90:4902-4906, 1993). Libraries of SH2 binding mimetics may be screened by the procedure of Songyang et al., Cell 72:767 15 778, 1993. See also by the procedure of Songyang et al., Current Biology 4:973-982, 1994), to test for the ability of a compound to act as a substrate or inhibitor of protein kinases. Accordingly, in one aspect, the present invention provides a method for inhibiting a phosphatase in a warm-blooded animal, where the 20 method comprises administering t(o the animal an amount of a compound of the present invention, where the amount is effective to inhibit the phosphatase. In Type 2 (non-insulin dependent) diabetes, tyrosine phosphatases (PTP-1 b) counter-balance the effect of activated insulin-receptor kinases and may represent important drug targets. In vitro experiments show 25 that injection of PTPase blocks insulin stimulated-phosphorylation of tyrosyl residues on endogenous proteins. Thus, compounds of the invention may be used to modulate insulin action in diabetes In another aspect, the present invention provides a method for inhibiting the binding of a phosphotyrosine residue in a first protein to an SH2 30 domain of a second protein. The method comprises contacting an amount of a compound of the present invention with a composition comprising the first and second protein. The amount is effective to mitigate the binding between the first and second protein that occurs via the SH2 domain of the second protein and the phosphotyrosine residue of the first protein. 59 WO 2004/108731 PCT/US2003/017188 Protease Inhibition In another aspect, the present invention provides a method for inhibiting a protease in a warm-blooded animal. The method comprises administering to the animal an amount of a compound of the present invention 5 as described herein. The amount is effective to inhibit a protease in the animal. In various embodiments: protease is a serine protease; the protease is a serine protease selected from thrombin, Factor X, Factor IX, Factor VII, Factor XI, urokinase, HCV protease, chymase tryptase and kallikrein; the protease is thrombin; the protease is Factor VII; and the protease is selected 10 from an aspartic, cysteine and metallo protease. With regard to protease inhibition, Cathepsin B is a lysosomal cysteine protease normally involved in proenzyme processing and protein turnover. Elevated levels of activity have been implicated in tumor metastasis (Sloane, B. F. et al., Cancer Metastasis Rev. 9:333-352, 1990), rheumatoid 15 arthritis (Werb, Z. Textbook of Rheumatology, Keller, W. N.; Harris, W. D.; Ruddy, S.; Sledge, C. S., Eds., 1989, W. B. Saunder Co., Philadelphia, Pa., pp. 300-321), and muscular dystrophy (Katunuma and Kominami, Rev. Physiol. Biochem. Pharmacol. 108:1-20, 1987). Calpains are cytosolic or membrane bound Ca + + - activated 20 proteases which are responsible for degradation of cytoskeletal proteins in response to changing calcium levels within the cell. They contribute to tissue degradation in arthritis and muscular dystrophy (see Wang and Yuen Trends Pharmacol. Sci. 15:412-419, 1994). Interleukin Converting Enzyme (ICE) cleaves pro-lL-1 beta to IL 25 1 beta, a key mediator of inflammation, and therefore inhibitors of ICE may prove useful in the treatment of arthritis (see, e.g., Miller B. E. et al., J. Immunol. 154:1331-1338, 1995). ICE or ICE-like proteases may also function in apoptosis (programmed cell death) and therefore play roles in cancer, AIDS, Alzheimer's disease, and other diseases in which disregulated apoptosis is 30 involved (see Barr and Tomei, Biotechnol. 12:487-493, 1994). HIV protease plays a key role in the life cycle of HIV, the AIDS virus. In the final steps of viral maturation it cleaves polyprotein precursors to the functional enzymes and structural proteins of the virion core. HIV protease inhibitors were quickly identified as an excellent therapeutic target for 35 AIDS (see Huff, J. R., J. Med. Chem. 34:2305-2314) and have already proven 60 WO 2004/108731 PCT/US2003/017188 useful in its treatment as evidenced by the recent FDA approval of ritonavir, Crixivan, and saquinavir. Hepatitis C virus (HCV) is the major cause of non-A and non-B hepatitis in the world today. It is estimated to infect up to 50 million people. 5 Currently there is no satisfactory treatment available to halt the progression of this debilitating disease. During the life cycle of the virus, a polyprotein of about 3000 amino acids is produced and is proteolytically cleaved by host and viral proteases to produce the mature viral gene products. A serine proteinase located within the HCV NS3 protein cleaves at four specific sites to 10 produce non-structural proteins considered essential for viral replication. Hence, inhibitors of HCV protease are attractive targets for drug design, and could be of great therapeutic benefit. (Neddermann et al., Biol. Chem. 378:469-476, 1997.) Angiotensin converting enzyme (ACE) is part of the renin 15 angiotensin system which plays a central role in the regulation of blood pressure. ACE cleaves angiotensin I to the octapeptide angiotensin II, a potent pressor agent due to its vasoconstrictor activity. Inhibition of ACE has proved therapeutically useful in the treatment of hypertension (Williams, G. H., N. Engl. J. Med. 319:1517-1525, 1989). 20 Collagenases cleave collagen, the major constituent of the extracellular matrix (e.g., connective tissue, skin, blood vessels). Elevated collagenase activity contributes to arthritis (Krane et al., Ann. N.Y Acad. Sci. 580:340-354, 1990.), tumor metastasis (Flug and Kopf-Maier, Acta Anat. Basel 152:69-84, 1995), and other diseases involving the degradation of connective 25 tissue. Trypsin-like serine proteases form a large and highly selective family of enzymes involved in hemostasis/coagulation (Davie and Fujikawa, Ann. Rev. 799-829, 1975) and complement activation (Muller-Eberhard, Ann. Rev. Biochem. 44:697-724,1975). Sequencing of these proteases has shown 30 the presence of a homologous trypsin-like core with amino acid insertions that modify specificity and which are generally responsible for interactions with other macromolecular components (Magnusson et al., Miami Winter Symposia 11:203-239, 1976). Thrombin, a trypsin-like serine protease, acts to provide limited 35 proteolysis, both in the generation of fibrin from fibrinogen and the activation of the platelet receptor, and thus plays a critical role in thrombosis and 61 WO 2004/108731 PCT/US2003/017188 hemostasis (Mann, K. G., Trends Biochem. Sci. 12:229-233,1987). Thrombin exhibits remarkable specificity in the removal of fibrinopeptides A and B of fibrinogen through the selective cleavage of only two Arg-Gly bonds of the one hundred and eighty-one Arg- or Lys-Xaa sequences in fibrinogen (Blomback, 5 Blood Clotting Enzymology, Seeger, W. H. (ed.), Academic Press, New York, 1967, pp. 143-215). Many significant disease states are related to abnormal hemostasis, including acute coronary syndromes. Aspirin and heparin are widely used in the treatment of patients with acute coronary syndromes. 10 However, these agents have several intrinsic limitations. For example, thrombosis complicating the rupture of atherosclerotic plaque tends to be a thrombin-mediated, platelet-dependent process that is relatively resistant to inhibition by aspirin and heparin (Fuster et al., N. Engl. J. Med. 326:242-50, 1992). 15 Thrombin inhibitors prevent thrombus formation at sites of vascular injury in vivo. Furthermore, since thrombin is also a potent growth factor which initiates smooth muscle cell proliferation at sites of mechanical injury in the coronary artery, inhibitors block this proliferative smooth muscle cell response and reduce restenosis. Thrombin inhibitors would also reduce 20 the inflammatory response in vascular wall cells (Harker et al., Am. J. Cardiol. 75:122-16B, 1995). Furthermore, at least two well-defined transcription factors, nuclear factor (NF) KB and activator protein (AP)-1, are regulated by the intracellular reduction-oxidation (redox) state. The regulation of gene 25 expression by the redox state holds promising therapeutic implications. For example, binding sites of the redox-regulated transcription factors NF-KB and AP-1 are located in the promoter region of a large variety of genes that are directly involved in the pathogenesis of diseases, such as AIDS, cancer, atherosclerosis and diabetic complications (Sen and Packer, FASEB Journal 30 10:709-720, 1996). More specifically, the binding of transcription factors such NF-KB and AP-1 to consensus sites on DNA is driven by oxidant-antioxidant homeostasis, especially by the thiol-disulfide balance. In the case of NF-KB, a physiologically relevant thiol that plays a crucial role in the regulation of NF-KB function is reduced thioredoxin or a 35 reduced thioredoxin-like protein. Thioredoxin is an important protein oxidoreductase with antioxidant functions. Thioredoxin has been found to 62 WO 2004/108731 PCT/US2003/017188 upregulate DNA binding of activated NF-KB and thus augments gene expression (Schenk et al., Proc. Natl. Acad. Sci. USA 91:1672-1676,1994). Thioredoxin has been implicated in reducing activated cytosolic NF-KB (specifically reduction of cys-62), which may thus contribute to its nuclear 5 translocation and DNA binding (Hayashi et at., J. Biol. Chem. 268:11380 11388, 1993). DNA binding activity of Fos and Jun in the AP-1 complex has also been found to be regulated by the redox state (Abate et al., Science 249:1157-1162, 1990). Each protein contains a single conserved cysteine 10 (flanked by lysine and arginine) in its DNA binding domain. This thiol does not appear to be part of a disulfide bond and may exist as a sulfenic or sulfinic acid in its oxidized form. Ref-1, a bifunctional nuclear protein also possessing endonuclease DNA repair activity, stimulates AP-1 DNA binding by reduction of this regulatory cysteine. A Fos mutant in which the critical cysteine was 15 replaced with serine elicited a three-fold increase in AP-1 DNA binding activity and was no longer subject to redox control (Okuno et al., Oncogene 8:695 701, 1993). Hence, since at least four members of the fos family, 3 of the jun family, and at least 4 of the ATF/CREB family of transcription factors all contain this conserved cysteine, redox control of transcription factors appears 20 widespread. As mentioned above, the regulation of transcription factors such as NF-KB and AP-1 have important therapeutic implications. For example, AP-1 is an important mediator of tumor production (Yoshioka et al., Proc. Natl. Acad. Sci. USA 92:4972-4976,1995). Thus, compounds that repress AP-1 25 transcriptional activity have utility in the treatment of cancer. Furthermore, due to its direct role in regulating responses to inflammatory cytokines and endotoxins, the activation of NF-KB plays an important role in the development of chronic diseases such as rheumatoid arthritis and acute conditions such as septic shock. Autoimmune diseases, such as systemic lupus erythromatus 30 (SLE), and Alzheimer's disease are also believed involved in activation of NF KB. Similarly, NF-KB plays an important role in the activation of HIV gene expression. Further conditions which are believed to involve NF-KB include the flu, atherosclerosis, oncogenesis and ataxia telangiectasia (AT). 63 WO 2004/108731 PCT/US2003/017188 Oxidoreductase Inhibition With respect to regulation of transcription factors, the compounds of this invention regulate transcription factors whose ability to bind to DNA is controlled by reduction of a cysteine residue by a cellular oxidoreductase. In 5 one embodiment, the transcription factor is NF-KB. In this embodiment, the compounds of this invention have activity as mediators of immune and/or inflammatory responses, or serve to control cell growth. In another embodiment, the transcription factor is AP-1, and the cellular oxidoreductase is Ref-1. In this embodiment, the compounds of this invention have activity as 10 anti-inflammatory and/or anticancer agents. In yet further embodiments, the transcription factor is selected from Myb and glucocorticoid receptor. Other transcription factors that may be regulated within the context of this invention also include: those of the NF-KB family, such as Rel-A, c-Rel, Rel-B, p50 and p52; those of the AP-1 family, such as Fos, FosB, Fra-1, Fra-2, Jun, JunB and 15 JunD; ATF; CREB; STAT-1, -2, -3, -4, -5 and -6; NFAT-1, -2 and -4; MAF; Thyroid Factor; IRF; Oct-1 and -2; NF-Y; Egr-1; and USF-43. Accordingly, in one aspect the present invention provides a method for inhibiting an oxidoreductase in a warm-blooded animal, comprising administering to the animal an amount of a compound of the present invention, 20 where the amount is effective to inhibit the oxidoreductase. Inhibition of oxidoreductase activity can be used as a means to regulate transcription. CAAX Inhibition In another aspect, the present invention provides a method for CAAX inhibition in a warm-blooded animal. The method comprises 25 administering to the animal an amount of a compound of the present invention as described herein. The amount is effective to provide CAAX inhibition in the animal. Ras, the protein product of the ras oncogene, is a membrane bound protein involved in signal transduction regulating cell division and 30 growth. Mutations in the ras gene are among the most common genetic abnormalities associated with human cancers (Barbacid, M. Annu Rev Biochem 56:779-827, 1987). These mutations result in a growth signal that is always "on," leading to a cancerous cell. In order to localize to the cell membrane, Ras requires prenylation of the cysteine within its C-terminal CAAX 35 sequence by farnesyl transferase (FTase) where, in the sequence CAAX, "a" is 64 WO 2004/108731 PCT/US2003/017188 defined as an amino acid with a hydrophobic side chain and "X" is another amino acid. This post-translational modification is crucial to its activity. Peptidyl inhibitors of FTase with the sequence CaaX have been shown to block or slow the growth of tumors in cell culture and in whole animals (Kohl et 5 al., Science 260:1934-1937, 1993; Buss and Marsters, Chemistry and Biology 2:787-791, 1995). Methods to screen for the activity of a compound to inhibit CAAX activity are known in the art. See, e.g., U.S. Patent No. 6,391,574, which describes a method of identifying a compound which inhibits the proteolytic 10 removal of an AAX tripeptide of a CAAX protein in a cell. See also U.S. Patent No. 5,990,277, which discloses several suitable assays, and references Gibbs et al., Cell 77:175,1994; Gibbs, Cell 65:1, 1991; Maltese, FASEB J. 4:3319, 1990; Moores et al., J. Biol. Chem. 266:14603, 1991; Goldstein et al., J. Biol. Chem. 266:15575, 1991; European Patent 0 461 869 A2; Casey, J. 15 Lipid Res. 33:1731-1740, 1992; Coxet al., Curr. Opin. Cell Biol. 4:1008-1016. 1992; Garcia et al., J. Biol. Chem. 268:18415-18418,1993; Vogt et al., J. Biol. Chem. 270:660-664, 1995; Kohl et al., Science, 260:1934-1937, 1993; Garcia et al., J. Biol. Chem., 268:18415-18418, 1993; and Vogt et al., J. Biol. Chem. 270:660-664, 1995). 20 MHC Molecules In another aspect, the present invention provides a method for inhibiting the binding of an antigenic peptide to either a class one or class two MHC molecule. The method comprises contacting a compound according to the present invention with a composition comprising an antigenic peptide and 25 either a class one or class two MHC molecule. The compound is contacted with the antigen/molecule in an amount effective to reduce the binding affinity between the two species. An important aspect of the immune system is the T cell response. This response requires that T cells recognize and interact with complexes of 30 cell surface molecules, referred to as human leukocyte antigens ("HLA"), or major histocompatibility complexes ("MHCs"), and peptides (see, e.g., Male et al., Advanced Immunology (J. P. Lipincott Company, 1987). Antigens mobilize an immune response, at least in part, by being ingested by an antigen-presenting cell (APC) which contains on its surface a Class II 35 glycoprotein encoded by a gene in the major histocompatibility complex 65 WO 2004/108731 PCT/US2003/017188 (MHC). The antigen is then presented to a specific T helper cell in the context of the surface bound MHC glycoprotein, and by interaction of the antigen specific T cell receptor with the antigen --MHC complex, the T helper cell is stimulated to mediate the antigen-specific immune response, including 5 induction of cytotoxic T cell function, induction of B cell function, and secretion of a number of factors aiding and abetting this response. In one aspect of the invention, the MHC molecule is HLA-A2.1, HLA-A1 or HLA-A3.1, or any other HLA allele that is present in melanoma patients. The ability of a compound of the present invention to bind to 10 MHC I molecules can be demonstrated essentially as described by Elliot et al., Nature 351:402-406, 1991. Similarly, the ability of a compound of the invention to bind to MHC 11 molecules can be demonstrated by the procedure of Kwok et al., J. Immunol. 155:2468-2476, 1995. Protein with 14-3-3 Domain 15 In another aspect, the present invention provides a method for inhibiting the binding of a first peptide to a second pepetide that comprises a 14-3-3 domain, where the first peptide has a binding affinity to the 14-3-3 domain of the second peptide. The method comprises contacting a compound of the present invention with a composition comprising a (first) 20 peptide that has a binding affinity to the 14-3-3 domain of the second protein. Proteins having the 14-3-3 domain, and binding partners thereof, have been described in the literature. These peptides may be used in the method of the present invention. See, e.g., Dai and Murakami, J Neurochem 2003 Jan. 84(1):23-34; Lim et al., J Biol Chem 2002 Oct. 25, 277(43):40997 25 1008; Parvaresch et al., FEBS Lett 2002 Dec. 18, 532(3):357-62; Eilers et al., Mol Cell Biol 2002 Dec. ;22(24):8514-26; Liu et al., Cancer Res 2002 Nov. 15, 62(22):6475-80; Truong et al., Proteins 2002 Nov. 15, 49(3):321-5; Birkenfeld et al., Biochem J 2003 Jan. 1, 369(Pt 1):45-54; Espejo et al., Biochem J 2002 Nov. 1, 367(Pt 3):697-702; and Benzing et al., J Biol Chem 2002 Sep 6, 30 277(36):32954-62. In the practice of the methods of this invention, a therapeutically effective amount of a compound of this invention is administered to a warm blooded animal in need thereof. For example, the compounds of this invention may be administered to a warm-blooded animal that has been 35 diagnosed with, or is at risk of developing, a condition selected from any one or 66 WO 2004/108731 PCT/US2003/017188 more of Chrohn's disease, asthma, rheumatoid arthritis, ischemia, reperfusion injury, graft versus host disease (GVHD), amyotrophic lateral sclerosis (ALS), Alzheimer's disease, allograft rejection and adult T-cell leukemia. Tuberous sclerosis complex 5 Patients having tuberous sclerosis complex (TSC) typically develop multiple focal lesions in the brain, heart, kidney and other tissues (see, e.g., Gomez, M.R. Brain Dev. 17(suppl):55-57, 1995). Studies in mammalian cells have shown that overexpression of TSC1 (which expresses hamartin) and TSC2 (which expresses tuberin) negatively regulates cell proliferation and 10 induces G 1 /S arrest (see, e.g., Miloloza, A. et al., Hum. MoL. Genet. 9:1721 1727, 2000). Other studies have shown that hamartin and tuberin function at the level of the 3 -catenin degradation complex, and more specifically that these proteins negatively regulate beta-catenin stability and activity by participating in the beta-catenin degradation complex (see, e.g., Mak, B.C., et 15 al. J. Biol. Chem. 278(8):5947-5951, 2003). Beta-catenin is a 95-kDa protein that participates in cell adhesion through its association with members of the membrane-bound cadherin family, and in cell proliferation and differentiation as a key component of the Wnt/Wingless pathway (see, e.g., Daniels, D.L., et al., Trends Biochem. Sci. 26:672-678, 2001). Disruption of this pathway has 20 been shown to be oncogenic in humans and rodents. The present invention provides compounds that modulate 13-catenin activity, and particularly its interactions with other proteins, and accordingly may be used in the treatment of TSC. The following examples are provided for purposes of illustration, 25 not limitation. EXAMPLES In the Preparation Examples and Examples, the following abbreviations are used: BMS : Boron dimethyl sulfide 30 CbzOSu : Benzyloxycarbonyl N-hydroxysuccinimide DIC : 1,3-Diisopropylcarbodiimide DIEA: N,N-Diisopropylethylamine DIPEA: N,N-Diisopropylethylamine DMAP : N,N-Dimethylaminopyridine 67 WO 2004/108731 PCT/US2003/017188 DMF: Dimethylformamide DMSO: Dimethyl sulfoxide EA: Ethyl acetate EDC: 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide. 5 hydrochloride EDCI: 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride FmocOsu : 9-Fluorenyloxycarbonyl N-hydroxysuccinimide HATU: [2-(1H-9-azabenzotriazole-1-yl)-l, 1,3,3 10 tetramethyluronium hexaflurophosphate] Hex. : Hexane HOBT: N-Hydroxybenzotriazole MC: Methylene chloride MeOH : Methanol 15 -OBn : -O-benzyl PPTS : Pyridinium p-toluenesulfonate PyBOP : Benzotriazole-1-yl-oxy-tris-pyrrolidino-phosphonium hexafluorophosphate p-TsOH : p-Toluenesulfonic acid 20 THF : Tetrahydrofuron TLC : Thin Layer Chromatography PREPARATIVE EXAMPLE 1 (1) PREPARATION OF NAPHTHALENE-2-CARBOXYLIC ACID AMIDE 0 0NH2 25 To a solution of 2-naphthoic acid (25 g, 0.145 mol) in MC (200 ml), oxalyl chloride (38 ml, 0.4356 mol) and a catalytic amount of DMF were added and stirred at room temperature for 2hrs. After the solvent was evaporated, the crude acyl chloride was diluted with MC (200 ml), to which a solution of ammonium hydroxide in water (160 ml) was dropwise added at an 30 ice bath temperature. After stirring for 1hr, the precipitated product was collected by suction filtration, triturated in hexane and dried to obtain the title compound, which was used next step without further purification. 68 WO 2004/108731 PCT/US2003/017188 (2) PREPARATION OF NAPHTHALENE-2-YL-METHYLAMINE NH 2 To a solution of the crude amide obtained in the above step (1) in THF (100 ml), BMS (27.5 ml, 0.2904 mol) was slowly added at 0 OC. The 5 resulted reaction mixture was heated to 60 oC for 3hrs, quenched with 5% HCI at 0 oC, extracted with EA and washed with 5% HCI. The aqueous layers were combined and basified with 1N NaOH, and again extracted with EA. The organic layers were combined and concentrated to give the title compound (13 g) as white solid. 10 TLC System 1 : MC/MeOH =90:10 v/v Rf=0.23 'H-NMR (300 MHz, CDCI 3 ) 8 ppm: 4.07(s, 2H), 7.48(m, 3H), 7.79(m, 4H) PREPARATIVE EXAMPLE 2 (1) PREPARATION OF I H-INDOLE-2-CARBOXYLIC ACID AMIDE N NH 2 15 H To a solution of indole-2-carboxylic acid (1 g, 6.21 mmol) in MC (30 ml), oxalyl chloride (1.64 ml, 0.18.62 mmol) and a catalytic amount of DMF were added and stirred at room temperature for 2hrs. After the solvent was evaporated, the crude acyl chloride was diluted with MC (20 ml), to which a 20 solution of ammonium hydroxide in water (7 ml) was dropwise added with cooling in an ice bath. After stirring for 1 hr, the precipitated product was collected by suction filtration, triturated in hexane and dried to give the title compound, which was used in the next step without further purification. 69 WO 2004/108731 PCT/US2003/017188 (2) PREPARATION OF (1H-INDOL-2-YL)-METHYLAMINE N NH 2 H To a solution of the crude amide obtained in the above step (1) in THF (30 ml), BMS (1.18 ml, 12.42 mmol) was slowly added at 0 OC. The 5 resulting reaction mixture was heated to 60 oC for 3hrs, quenched with 5% HCI at 0 0C, extracted with EA, and washed with 5% HCI. The aqueous layers were combined and basified with 1N NaOH, and again extracted with EA. The organic layers were combined and concentrated to obtain the title compound (0.28 g) as yellow oil. 10 TLC System 1 : MC/MeOH =90:10 v/v Rf=0.15 1 H-NMR (300MHz, CDC 3 ) 8 ppm: 3.98(s, 2H), 7.08(m, 3H), 7.26(m, 1H), 7.58(d, 1H), 9.10(br s, 1H) PREPARATIVE EXAMPLE 3 (1) PREPARATION OF 2-BENZYLOXYCARBONYLAMINO-4-OXO-BUTYRIC ACID BENZYL 15 ESTER O NHCbz H OBn O 0 To a solution of Z-Asp-OBn (10 g, 0.028 mol) in MC (200 ml), oxalyl chloride (2.93 ml, 0.0336 mol) and a catalytic amount of DMF were added at 0 oC and stirred at room temperature for 2hrs. After the solvent was 20 evaporated, the crude acyl chloride was dissolved in benzene (400 ml), and tributyltin hydride (15.1 ml, 0.056 mol) and a catalytic amount of Pd(0) were added slowly at 0 oC and stirred at room temperature overnight. After the solvent was evaporated, ether (100 ml)/10% KF in water (100 ml) was added and stirred at room temperature for 2hrs, followed by filtration to give a 25 biphasic solution. The organic layer was separated and concentrated to give a crude product, which was purified by column chromatography to obtain the title compound, Z-Asp-OBn aldehyde (6 g) as pale-yellow oil. Rf: 0.29 in Hexane/EA (2/1) 70 WO 2004/108731 PCT/US2003/017188 (2) PREPARATION OF 2-BENZYLOXYCARBONYLAMINO-4,4-DIMETHOXY-BUTYRIC ACID BENZYL ESTER O NHCbz O OBn O 0 To a solution of Z-Asp-OBn aldehyde (6 g, 17.58 mmol) obtained 5 in the above step (1) in MeOH (100 ml), a catalytic amount of p-TsOH was added and stirred at room temperature for 5hrs. After the reaction was complete, the solvent was evaporated to give a crude product, which was purified by column chromatography to obtain the title compound, Z-Asp-OBn acetal, (5 g) as pale-yellow oil. 10 Rf: 0.32 in Hex./EA (2/1) (3) PREPARATION OF 2-BENZYLOXYCARBONYLAMINO-4,4-DIMETHOXY-BUTYRIC ACID O NHCbz ~OH O 0 The Z-Asp-OBn acetal (0.5 g, 1.29 mmol) obtained in the above step (2) was dissolved in THF (20 ml)/NaOH (0.11 g, 2.1 mmol) in water (20 15 ml) and stirred at room temperature for 30min. After the starting material had disappeared completely, the reaction mixture was concentrated by evaporation and then diluted with water/EA. The aqueous layer was separated, acidified very carefully to pH 4-5 with 1N HCI at 0 oC, and again extracted with EA. The organic layers were combined and concentrated to obtain the title 20 compound, Z-Asp-OBn acetal, (0.27 g) as pale-yellow oil. TLC System 1 : Hexane/EA =20:1Ov/v Rf=0.10 1 H-NMR (300MHz, CDCI 3 ) 8 ppm: 2.20(s, 2H), 3.35(d, 6H), 4.52(m, 2H), 5.19(t, 2H), 5.80(d, 1H), 7.37(br s, 5H) 71 WO 2004/108731 PCT/US2003/017188 (4) PREPARATION OF 2-AMINO-4,4-DIMETHOXY-BUTYRIC ACID O NH 2 O-- OH OH 0 In a reaction vessel equipped with a hydrogen gas balloon, a solution of the Z-Asp-OBn acetal (2.22 g, 5.73 mmol) obtained in the above 5 step (3) in acetic acid (20 ml) and Pearlman's catalyst was added and stirred at room temperature overnight. The resulting reaction mixture was filtered, concentrated and lyophilized to give a crude product, which was used in the next step without further purification. (5) PREPARATION OF 2-(9H-FLUOREN-9-YLMETHOXYCARBONYLAMINO)-4,4 10 DIMETHOXY-BUTYRIC ACID O HN, Fmoc -O, OH O 0 To a solution of the crude Asp-OH acetal obtained in the above step (4) in THF (100 ml)/water (100 ml), FmocOsu (2.13 g, 6.3 mmol)/sodium bicarbonate (1.93 g, 22.92 mmol) was added and stirred at room temperature 15 overnight. The resulting reaction mixture was concentrated and diluted with water/EA. The aqueous layer was separated, acidified very carefully to pH 4 5 with 1N HCI at 0 oC, and again extracted with EA. The organic layers were combined and concentrated to give a crude product, which was purified by column chromatography to obtain the title compound (1.5 g) as a foamy solid. 20 Rf : 0.15 in Hex./EA (2/1) 72 WO 2004/108731 PCT/US2003/017188 PREPARATIVE EXAMPLE 4 (1) PREPARATION OF 2-BENZYLOXYCARBONYLAMINO-PENTADIOIC ACID 0 0 HO OH NHCbz To a solution of L-glutamic acid (20 g, 136 mmol) in water/THF 5 (1/1, 400 ml), sodium bicarbonate (45.7 g, 544 mmol) was added and cooled to 0 OC in an ice bath. To the reaction mixture, CbzOSu (37.3 g, 150 mmol) was added and stirred overnight at room temperature. After the reaction was completed, the resulting reaction mixture was extracted with EA. The aqueous layer was separated, acidified to pH 2 with conc. HCI at 0 oC, and 10 again extracted with EA (4 times). The organic layers were concentrated to give a crude product, which was purified by column chromatography to obtain the title compound (16 g) as colorless oil. Rf: 0.2 in MC/MeOH (9/1) (2) PREPARATION OF 4-(2-CARBOXY-ETHYL)-5-OXO-OXAZOLIDINE-3-CARBOXYLIC 15 ACID BENZYL ESTER O 0 HO o N-_I Cbz In a Dean-Stark apparatus, N-Cbz-L-glutamic acid (4 g, 14.22 mmol) obtained in the above step (1), paraformaldehyde (5 g), a catalytic amount of pTsOH, molecular sieves (5 g), and toluene (100 ml) were placed 20 and refluxed until the starting material disappeared. The resultant reaction mixture was cooled to room temperature, filtered and concentrated to give a crude product, which was purified by column chromatography to obtain the title compound (2 g) as colorless oil. Rf: 0.45 in only EA 73 WO 2004/108731 PCT/US2003/017188 (3) PREPARATION OF 5-Oxo-5-(3-Oxo-PROPYL)-OXAZOLIDINE -3-CARBOXYLIC ACID BENZYL ESTER 0 o H 1 'o Cbz To a solution of the di-protected glutamic acid (2 g, 6.82 mmol) 5 obtained in the above step (2) in MC (200 ml), oxalyl chloride (0.7 ml, 7.5 mmol) and a catalytic amount of DMF were added at 0 oC and stirred at room temperature for 2hrs. After the solvent was evaporated, the resultant crude acyl chloride was dissolved in THF (400 ml), to which tributyltin hydride (3.86 ml, 14.34 mmol) and a catalytic amount of Pd (0) were slowly added at 0 OC 10 and stirred at room temperature overnight. After the solvent had been evaporated, ether (100 ml)/10% KF in water (100 ml) was added and stirred at room temperature for 2hrs, followed by filtration to give a biphasic solution. The organic layer was separated and concentrated to give a crude product, which was purified by column chromatography to obtain the title compound 15 (0.7 g) as colorless oil. Rf : 0.23 in hexane/EA (4/1) (4) PREPARATION OF 4-(3,3-DIMETHOXY-PROPYL)5-OXO-OXAZOLIDINE-3 CARBOXYLIC ACID BENZYL ESTER O o Cbz 20 To a solution of di-protected aldehyde (0.7 g, 2.53 mmol) obtained in the above step (3) in MeOH (30 ml), a catalytic amount of pTsOH was added and stirred at room temperature for 7hrs. After the reaction was complete, the reaction mixture was concentrated by evaporation of solvent to give a crude product, which was purified by column chromatography to obtain 25 the title compound (0.5 g) as colorless oil. Rf: 0.33 in Hexane/EA (4/1) 74 WO 2004/108731 PCT/US2003/017188 (5) PREPARATION OF 2-BENZYLOXYCARBONYLAMINO-5,5-DIMETHOXY-PENTANOIC ACID 0 0 OOH O N OH Cbz N H The diprotected acetal (0.456 g, 1.411 mmol) obtained in the 5 above step (4) was dissolved in MeOH (20 ml)/1N NaOH (10 ml) and stirred at room temperature overnight. After the starting material had disappeared completely, the reaction mixture was concentrated by evaporation of solvent and diluted with water/EA. The aqueous layer was separated, acidified very carefully to pH 4-5 with 1N HCI at 0 oC, and again extracted with EA. The 10 organic layers were combined and concentrated to obtain the title compound (0.35 g) as colorless oil. Rf : 0.1 in Hex./EA (1/1) (6) PREPARATION OF 2-AMINO-5,5-DIMETHOXY-PENTANOIC ACID OOH O OH NH 2 15 In a reaction vessel equipped with a hydrogen gas balloon, a solution of the Cbz-acetal (0.35 g, 1.13 mmol) obtained in the above step (5) in MeOH (10 ml) and a catalytic amount of 10%Pd/C was added and stirred at room temperature overnight. The resultant reaction mixture was filtered and concentrated to give a crude product (0.2 g) as colorless oil, which was used in 20 the next step without further purification. Rf : 0.01 in Hex./EA (1/1) 75 WO 2004/108731 PCT/US2003/017188 (7) PREPARATION OF 2-(9H-FLUOREN-9-YLMETHOXYCARBONYLAMINO)-5,5 DIMETHOXY-PENTANOIC ACID o o O OH Fmoc, NH To a solution of the crude Glu-OH acetal obtained in the above 5 step (6) in THF (10 ml)/water (10 ml), FmocOsu (0.42 g, 1.24 mmol)/sodium bicarbonate (0.5 g, 5.9 mmol) was added and stirred at room temperature overnight. After solvent was evaporated, the resultant reaction mixture was diluted with water/EA. The aqueous layer was separated and acidified very carefully to pH 4-5 with 1N HCI at 0 oC, and again extracted with EA. The 10 organic layers were combined and concentrated to obtain the title compound (0.19 g) as colorless oil. TLC System 1 : only EA Rf=0.25 1 H-NMR (300MHz, CDCI 3 ) 8 ppm: 1.75(br m, 4H), 3.28(d, 6H), 3.43(q, 1H), 4.20(t, 1H), 4.38(m, 3H), 5.62(d, 1 H), 7.31(m, 4H), 7.65(d, 2H), 15 7.75(d, 2H) PREPARATIVE EXAMPLE 5 (1) PREPARATION OF 2-TERT-BUTOXYCARBONYLAMINO-4-METHOXYCARBONYL AMINO-BUTYRIC ACID O HN 0 BocHN OH 0 20 To a solution of Boc-Dab-OH (3 g, 13.75 mmol) in H 2 0 (50mL), NaOH (2.75 g, 68.75 mmol, 5 equiv.) was slowly added until pH >11, to which methyl chloroformate (2.6 g, 27.5 mmol, 2 equiv.) in toluene (50 mL) was added. The resultant reaction mixture was stirred for 2hrs. For the TLC checking, a small amount of aqueous phase was taken out and acidified with 25 1N HCI. After confirming the reaction completion by TLC, the organic phase was separated and the aqueous phase was acidified with 10% HCI solution 76 WO 2004/108731 PCT/US2003/017188 and extracted by EA (5 mL X 2). The organic phases were combined, dried over anhydrous Na 2 SO 4 and concentrated in vacuo to give a crude product (3.277 g, 11.86 mmol, 86%) as a colorless oil. TLC System: EA Rf = 0.2 5 'H-NMR (300MHz, CDCl 3 ) 8 ppm: 1.30 ~ 1.50 (bs, 9H), 2.00 2.30 (m, 2H), 3.10 ~ 3.30 (m, 2H), 3.70 (bs, 3H), 4.35 (m, 1 H), 5.40 (m, 1 H), 5.65 (bs, 1H). (2) PREPARATION OF (1 -BENZYLCARBAMOYL-3-METHOXYCARBONYLAMINO-PROPYL) CARBAMIC ACID TERT-BUTYL ESTER O HN 0O H BocHN N 10 o To a solution of 2-tert-Butoxycarbonylamino-4 methoxycarbonylamino-butyric acid (1.1 g, 3.98 mmol) obtained in the above step (1) in DMF (20mL), EDCl (763mg, 3.98 mmol, 1 equiv.), HOBT (538mg, 3.98 mmol, 1 equiv.) and DIEA (1.4mL, 7.96 mmol, 2 equiv.) were added at 15 5 oC and stirred for 1 day. After the confirming the reaction completion by the TLC checking, the reaction solution was acidified by 10% HCI at 5 oC (until pH ~ 4) and extracted by EA (20mL). The organic phases were combined and washed with sat NaHCO 3 and brine, dried over anhydrous Na 2 SO 4 and concentrated in vacuum to give a residue, which was solidified by adding EA 20 and n-Hexane and purified by column chromatography to obtain the title compound (620 mg, 1.7 mmol, 43%) as a white solid. Rf = 0.7 (EA) 'H-NMR (300MHz, CDCi 3 ) 6 ppm: 1.45 (bs, 9H), 1.75 ~ 2.10 (m, 2H), 3.05 (m, 1H), 3.45 (m, 1H), 3.65 (s, 3H), 4.25 (m, 1H), 4.45 (d, 2H, J = 5.7 25 Hz), 5.45 (m, 1H), 7..05 (m, 1H), 7.20 - 7.45 (m, 5H). 77 WO 2004/108731 PCT/US2003/017188 (3) PREPARATION OF (3-AMINO-3-BENZYLCARBONYL-PROPYL)-CARBAMIC ACID TERT BUTYL ESTER HYDROCHLORIDE O HN-A 0 H HCI H 2 0 To a solution of (1-Benzylcarbamoyl-3-methoxycarbonylamino 5 propyl)-carbamic acid tert-butyl ester (1 g, 2.7 mmol) obtained in the above step (2) in 1,4-dioxane (10mL), 4N HCI in 1,4-dioxane (6.8mL, 27 mmol) were added and stirred for 2 hours. After the confirming the reaction completion by the TLC checking, the reaction solution was concentrated and dried in vacuum to afford the title compound as a white solid. 10 EXAMPLE 1 1-BENZYL-7-METHYL-6-THIOXO-HEXAHYDRO-PYRIMIDO[1,6-A]PYRIMIDIN-2-ONE (A) Preparation of N-benzyl-3-3-(2-[1,31dioxolan-2-yl-ethyl)-3methyl thioureidol-propionamide 0 S 0 O N N N | H H 15 A suspension of 13-alanine benzylamido hydrochloride (1.0 eq) and N-methylmorpholine (2.2 eq) in dichloromethane was treated with thiophosgene (1.2 eq) at 0°C for 10 min. The reaction mixture was allowed to warm to room temperature and stirred for additional 2 hrs. The clear solution was diluted with ethyl acetate and washed with 10% KHSO 4 solution, distilled 20 water, and sat. NaCl solution. The organic layer was dried over Na 2 SO 4 and concentrated to give an oily residue. This product was dissolved in dichloromethane and treated with 2-(N-methyl-2aminoethyl)-1,3-dioxolane (0.9 eq) at 00C for 10 min. The reaction mixture was allowed to warm to room temperature and stirred for 25 additional 4 hrs. The reaction was diluted with ethyl acetate and washed with 10% KHSO 4 solution, distilled water, sat. NaHCO 3 solution, distilled water, and 78 WO 2004/108731 PCT/US2003/017188 sat. NaCI solution. The organic layer was dried over Na 2 SO 4 and concentrated to give an oily residue. This crude product was purified by column chromatography (silica gel, ethyl acetate/hexane = 5/2) to give the pure product. 5 ' 1 H-NMR (500 MHz, CDCI 3 ) 8 ppm; 2.02 (mn, 2H), 2.60(m, 2H), 3.18(s, 3H), 3.82(m, 2H), 3.88(m, 2H), 4.03(m, 4H), 4.44(m, 2H), 4.91(m, 1H), 6.84(br.s, 1H), 7.25-7.38(m, 5H); MS (m/z, ESI), 352(MH ) (B) Preparation of 1-benzyl-7-methyl-6-thioxo-hexahydro-pyrimidof1,6 10 ajpyrimidin-2-one /N O N N S Amide obtained in the above step (A) was treated with formic acid at 601C for 4 days. After evaporation of formic acid under reduced pressure, the residue was purified by preparative TLC (silica gel, ethyl 15 acetate/methanol = 5/1) to give the pure title product. 'H-NMR (500 MHz, CDCI 3 ) 8 ppm: 2.05(m, 1H), 2.36(m, 1H), 2.64(d, 1H), 2.96(m, 1H), 3.30(m, 3H), 3.44(s, 3H), 4.42(d, 1H), 4.86(br.s, 1H), 5.08(d, 1H), 5.49(m, 1H) 7.25-7.38(m, 5H); MS (mlz, ESI), 290(MH*), 311(M Na) 79 WO 2004/108731 PCT/US2003/017188 EXAMPLE 2 1,7-DIBENZYL-6-THIOXO-HEXAHYDRO-PYRIMIDO[1,6-A]PYRIMIDIN-2-ONE (A) Preparation of N-benzyl-3-[3-benzyl-(3,3-diethoxy-propyl)-thioureido] propionamide O S 0 N--N N H H 5 & A suspension of 3-alanine benzylamido hydrochloride (1.0 eq) and N-methylmorpholine (2.2 eq) in dichloromethane was treated with thiophosgene (1.2 eq) at 0oC for 10 min. The reaction mixture was allowed to warm to room temperature and stirred for additional 2 hrs. The clear solution 10 was diluted with ethyl acetate and washed with 10% KHSO 4 solution, distilled water, and sat. NaCI solution. The organic layer was dried over Na 2 SO 4 and concentrated to give an oily residue. This product was dissolved in dichloromethane and treated with 2-(N-benzyl-l-amino-3,3-diethoxy propane (0.9 eq) at 0 0 C for 10 min. The reaction mixture was allowed to warm to room 15 temperature and stirred for additional 6 hrs. The resulting reaction mixture was diluted with ethyl acetate and washed with 10% KHSO 4 solution, distilled water, sat. NaHCO 3 solution, distilled water, and sat. NaCI solution. The organic layer was dried over Na 2 SO 4 and concentrated to give an oily residue. This crude product was purified by column chromatography (silica gel, ethyl 20 acetate/hexane = 2/1) to give the pure title product. 1 H-NMR (400 MHz, CDCl 3 ) 8 ppm; 1.22(t, 6H), 1.95(m, 2H), 2.60(m, 2H), 3.46(m, 2H), 3.60(br. t, 2H), 3.63(m, 2H), 3.97(m, 2H), 4.38(m, 2H), 4.52(m, 1H), 5.07((br.s, 2H), 6.16(br.s, 1H), 6.98(br.s, 1H), 7.25-7.38(m, 1 0OH); 25 MS (m/z, ESI), 458(MH') 80 WO 2004/108731 PCT/US2003/017188 (B) Preparation of 1,7-dibenzyI-6-thioxo-hexahydro-pyrimido[1,6 alpyrimidin-2-one NNO N YN S Amide obtained in the above step (A) was treated with formic 5 acid at 60 0 C for 4 days. After evaporation of formic acid under reduced pressure, the residue was purified by preparative TLC (silica gel, ethyl acetate/methanol = 5/1) to give the pure product. 'H-NMR (500MHz, CDCI 3 ) 8 ppm: 1.94(m, 1H), 2.24(m, 1H), 2.62(m, 1H), 3.01(m, 1H), 3.18(m, 1H), 3.43(m, 1H), 3.62(m, 1H), 4.39(d, 1H), 10 4.51(m, 1H), 4.91(m, 1H), 5.02(d, 1H), 5.26(d, 1H), 5.53(m, 1H), 7.25-7.40(m, 1 OH); MS (m/z, APCI), 366(MH') EXAMPLE 3 1,7-DIBENZYL-HEXAHYDRO-PYRIMIDO[1,6-A]PYRIMIDIN-2,6-DIONE 15 (A) Preparation of N-benzyl-3-[3-benzyl-(3,3-diethoxy-propyl)-thioureidol propionamide O O O0 H H A suspension of 13-alanine benzylamido hydrochloride (1.0 eq) and N-methylmorpholine (3.2 eq) in dichloromethane was treated with 20 triphosgene (0.7 eq) at 0 0 C for 10 min. The reaction mixture was allowed to warm to room temperature and stirred for additional 2 hrs. The clear solution was diluted with ethyl acetate and washed with 10% KHSO 4 solution, distilled 81 WO 2004/108731 PCT/US2003/017188 water, and sat. NaCI solution. The organic layer was dried over Na 2 SO 4 and concentrated to give an oily residue. This product was dissolved in dichloromethane and treated with 2-(N-benzyl-1l-amino3,3-diethoxy propane(0.9 eq) at 0 0 C for 10 min. The reaction mixture was allowed to warm 5 to room temperature and stirred for additional 4 hrs. The resulted reaction mixture was diluted with ethyl acetate and washed with 10% KHSO 4 solution, distilled water, sat. NaHCO 3 solution, distilled water, and sat. NaCI solution. The organic layer was dried over Na 2 SO 4 and concentrated to give an oily residue. This crude product was purified by column chromatography (silica 10 gel, ethyl acetate/hexane = 2/1) to give the pure title product. 'H-NMR (400 MHz, CDCl 3 ) 8 ppm: 1.23(t, 6H), 1.87(m, 2H), 2.55(m, 2H), 3.24(m, 2H), 3.49(m, 2H), 3.59(m, 2H), 3.65(m, 2H), 4.45-4.58(m, 5H), 5.62(br.s, 1H), 6.57(br.s, 1H), 7.25-7.48(m, 10H); MS (m/z, ESI), 442(MH') 15 (B) Preparation of 1,7-dibenzyl-hexahydro-pyrimidof 1,6-alpyrimidin-2,6 dione N 0 N 0 Amide obtained in the above (A) was treated with formic acid at 60 0 C for 4 days. After evaporation of formic acid under reduced pressure, the 20 residue was purified by preparative TLC (silica gel, ethyl acetate) to give the titled compound. 1 H-NMR (400MHz, CDCi 3 ) 8 ppm; 1.89(m, 1H), 2.19(m, 1H), 2.58(m, 1H), 2.75(m, 1H), 3.02(m, 3H), 4.42(d, J=12.4Hz, 1H), 4.55(d, J=2.4Hz, 2H), 4.65(m, 1H), 4.78(m, 1H), 4.98(d, J=12.4Hz, 1H), 7.25-7.38(m, 25 10OH); MS (mlz, ESI), 350(MH +) 82 WO 2004/108731 PCT/US2003/017188 EXAMPLE 4 1, 7 -DIBENZYL-6-OXO-OCTAHYDRO-PYRIMIDO[1,6-A]PYRIMIDIN-2-ONE (A) Preparation of (3-Bromo-l-methoxypronpan-l-oxy)-Linked ArqoGel Resin OMe 5 0 Br 5 A suspension of dry ArgoGel resin and pyridinium para toluensulphonate (240 mg, 0.96 mmol) in 1,2-dichloroethane (15 mL) was heated to reflux while continuously removing the solvent and traces of water. After removing about 5mL of the distillate, a solution of 3-bromo-1,1 10 dimethoxypropane (700 mg, 3.84 mmol) in 1,2-dichloroethane (5mL) was added and the mixture was kept at reflux for 4h with continuous removal of EtOH/EDC, after which the resin was washed with DMF and dioxane followed by lyophilization to give the desired product. (B) Preparation of (3-benzylamino-1l-methoxypropan-1l-oxy)-linked ArqoGel 15 resin OCH 3 H A solution of benzyl amine (520 mg, 4.85 mmol) in DMSO (4 mL) was added to the bromoacetal resin (1 g, 0.48 mmol) and the suspension was shaken at 60 0 C for 15 hrs. The resulted resin was filtered, washed with 20 DMSO, MeOH and MC, and dried in vacuo overnight. The secondary amine was detected by chloranil test. 83 WO 2004/108731 PCT/US2003/017188 (C) Preparation of -alanine benzyl amine urea OCH 3 Q o N NHBn 0 To a solution of 3-alanine benzyl amide HCI (80 mg, 0.36 mmol) in N-methyl morpholine (120 pl) and MC (2 mL), triphosgene (0.72 mmol) was 5 added at room temperature. After 10 minutes, the resulted isocyanate solution was added to a suspension of the secondary amine resin obtained in above step (2) (100 mg, 0.048 mmol) and kept shaking for 3 hrs at room temperature. The resin was washed with DMF, MeOH and MC, and the completion of reaction was checked with chloranil test. 10 (D) Preparation of 1,7-dibenzyl-6-oxo-octahydro-pyrimido[1,6-alpyrimidin-2 one Bn I N"/. N 0 Bn O The thiourea group-containing resin of step (C) was treated with formic acid and kept shaking for 15 hrs. The resin was filtered off and the 15 filtrate was concentrated and purified by chromatography (silica gel) to obtain the title compound. 1 H-NMR (500MHz, CDCI 3 ) 8 ppm: 1.94(m, 1H), 2.24(m, 1H), 2.62(m, 1H), 3.01(m, 1H), 3.18(m, 1H), 3.43(m, 1H), 3.62(m, 1H), 4.39(d, 1H), 4.51(m, 1H), 4.91(m, 1H), 5.02(d, 1H), 5.26(d, 1H), 5.53(m, 1H), 7.25-7.40(m, 20 10H); MS (m/z, APCI), 366(MH ) 84 WO 2004/108731 PCT/US2003/017188 EXAMPLE 5 1,7-DIBENZYL-2-oxo-6-THIOXO-OCTAHYDRO-PYRIMIDO[1,6-A]PYRIMIDINE-4 CARBOXYLIC ACID BENZYL ESTER (A) Preparation of 2-isothiocyanato-succinic acid 1-benzyl ester 4-(9H 5 fluoren-9-ylmethyl) ester CO 2 Bno SCN OFm To a solution of 2-tert-butoxycarbonylamino-succinic acid 1 benzyl ester (1 g, 3.09 mmol) in MC, DIC (532 pl, 1.1 eq), DMAP (188 mg, 0.5 eq) and fluorenyl methanol (635 mg, 1.05 eq) were added. After the 10 reaction was completed, the resultant reaction mixture was washed with 1 N HCI and sat. NaHCO 3 solution, and purified by column chromatography (silica gel) to obtain the fluorenyl methyl ester (400 mg). This ester was diluted in dioxane (10 ml) and a 4N HCI solution of dioxane was added and kept stirring for 2 hrs to remove the Boc protection 15 group. After completion of the reaction, the solution was evaporated to dryness. The HCI salt of the amine was diluted with MC and N-methyl morpholine, and thiophosgene (1.2 eq) was added at ca. 0 0 C. After the reaction was complete, the mixture was washed with 10% KHSO 4 solution, distilled water, sat. NaHCO 3 solution, distilled water, and sat. NaCI solution. 20 The organic layer was dried over Na 2 SO 4 and concentrated to give an oily residue. This crude product was purified by column chromatography (silica gel, ethyl acetate/hexane = 111) to give the pure title product. (C) Aspartic acid benzyl,fluorenyl ester thiourea 0 ,-Ph OY-NH BnO 2 C OFm 0 25 A MC solution of the isocyanate (0.5 rmmol) obtained in the above step (A) with N-methyl morpholine was added to a suspension of the 85 WO 2004/108731 PCT/US2003/017188 secondary amine resin (200 mg, 0.04 mmol) as obtained in step (B) of Example 4 and kept shaking for 3 hrs at room temperature. The resultant resin was washed with DMF, MeOH and MC, and the completion of reaction was checked with chloranil test. 5 (C) Aspartic acid thiourea benzylamide I _ /--Ph O/.. N s-NH BnO 2 C -ONHBn The resin obtained in the above step (C) was swelled for 30 min in DMF (4 mL), and a 25% piperidine solution was added to cleave the fluorenyl methyl protection. The resultant resin was washed with DMF, MeOH 10 and MC. The resin was dried under reduced pressure and swelled again, to which DIC (8 pL, 0.05 mmol), HOBt (8 mg, 0.05 mmol) and DIEA (18 lpL, 0.1 mmol) were added to activate the acid. After shaking for 30 min, benzyl amine was added and kept shaking overnight to obtain the desired benzyl amide resin. 15 (D) Preparation of 1,7-dibenzyl-2-oxo-6-thioxo-octahydro-pyrimidofl,6 alpyrimidine-4-carboxylic acid benzyl ester Bn N N Bn S CO 2 Bn The resin obtained in the above step (C) was swelled in MC (4 mL), to which PPTS (10 mg) was added and heated for 4 hrs at 60C0 to 20 obtain the title compound. MS (m/z, ESI), 500 (MH ). 86 WO 2004/108731 PCT/US2003/017188 EXAMPLE 6 7-BENZYL-6-THIOXO-HEXAHYDRO-PYRIMIDO[1,6-A]PYRIMIDINE-1 -CARBOXYLIC ACID BENZYL ESTER (A) Preparation of {3-[3-benzyl-3-(3,3-diethoxy-propyl)-thioureidol-propyll} 5 carbamic acid benzyl ester OEt "'O]Et 'S SN---NHCbz H A suspension of Cbz-diamino propane HCI (1.0 eq) and N methylmorpholine (2.2 eq) in MC was treated with thiophosgene (1.2 eq) at 0 0 C for 10 min. The resulting solution was allowed to warm to room 10 temperature and stirred for additional 2 hrs. The resulting clear solution was diluted with ethyl acetate and washed with 10% KHSO 4 , water, and sat. NaCI. The organic layer was dried over Na 2 SO 4 and concentrated to give an oily residue, which was dissolved in MC and treated with N-benzyl-1-amino-3,3 diethoxy propane (0.9 eq) at 00C for 10 min, and then allowed to warm to room 15 temperature and stirred for additional 6 hrs. The resulting reaction mixture was diluted with ethyl acetate and washed with 10% KHSO 4 solution, water, sat. NaHCO 3 , water, and sat. NaCl. The organic layer was dried over Na 2 SO 4 and concentrated to give an oily residue, which was then purified by column chromatography (silica gel, ethyl acetate/hexane, 2/1) to give the title 20 compound. 1 H-NMR (400 MHz, CDCI 3 ) 6 ppm: 1.17 (t, 6H), 1.5 (bs, 2H), 1.75(t, 2H), 1.92(m, 2H),3.20 (q, 2H), 3.45 (m, 2H), 3.60 (m, 4H), 3.75 (q, 2H), 4.51 (t, 1H), 5.06 (s, 4H), 6.75 (br.s, 1H), 7.25-7.38 (m, 10H); MS (m/z, ESI), 442 (M-OEt*). 87 WO 2004/108731 PCT/US2003/017188 (B) Preparation of 7bBenzvl-6-thioxo-hexahydro-pyrimidorl 1 ,6-alp y rimid in e 1-carboxylic acid benzyl ester Cbz I N N N S To a solution of the amide obtained in the above step (A) in MC, 5 PPTS was added and stirred at 70 0 C overnight. The resulting reaction mixture was concentrated under a reduced pressure to give a residue, which was purified by preparative TLC (ethyl acetate only) to obtain the title compound. 1 H-NMR (400MHz, CDCI 3 ) 8 ppm: 1.89(m, 2H), 1.95(m, 1H), 10 2.63(m, 1H), 2.80(m, 1H), 3.10(m, 1H), 3.45(m, 1H), 3.89(m, 1H), 4.01(m, 1H), 4.39(d, 1H), 4.51(m, 1H), 4.92(m, 2H), 5.10(m, 2H), 7.16- 7.4(m, 10OH); MS (m/z, ESI): 396(MH') EXAMPLE 7 8-ACETYL-6-OXO-HEXAHYDRO-PYRAZINO[1,2-A]PYRIMIDINE-1 15 CARBOXYLIC ACID BENZYL ESTER (A) Preparation of [acetyl-(2,2-dimethoxy-ethyl)-aminol-acetic acid 0= -0 N OH -0 0 To a solution of benzyl glycine HCI salt (1 eq) in MeOH, dimethoxy acetaldehyde (1.05 eq) and then NaCNBH 3 (1.2 eq) were added at 20 room temperature and stirred for 5 hrs. The resulting reaction mixture was concentrated under reduced pressure to give an oily residue, which was dissolved in MC and washed with sat. NaHCO 3 solution, water, and sat. NaCI solution. The organic layer was dried over Na 2 SO 4 and concentrated to give an oily residue, which was dissolved in MC and treated with triethyl amine 25 (3eq) and acetyl chloride (1.leq) at 0 0 C. 88 WO 2004/108731 PCT/US2003/017188 After the reaction was complete, the resulting reaction mixture was washed with sat. NaHCO 3 solution, water, and sat. NaCl solution. The organic layer was dried over Na 2 SO 4 and concentrated to give an oily residue, which was purified by column chromatography (silica gel, ethyl acetate) to give 5 the pure product. This product was hydrogenolyzed with 10% Pd/C and an H 2 -containing balloon to obtain the title compound, which was used in the next step without further purification. 1 H-NMR (400MHz, CDCI 3 ) 6 ppm: 2.09(s, 1H), 2.20 (s, 2H), 3.40 (d, 6H), 3.48 (d, 2H), 4.16 (s, 2H), 4.44 (m, 1H) 10 (B) Preparation of (3-{2-[acetyl-(2,2-dimethoxy-ethyl)-aminol-acetylamino} propyl)-carbamic acid benzyl ester 0 -0 N NH -0 0 NHCbz To a solution of the acid (1 eq) obtained in the above step (A) in MC, HATU (1 eq), DIPEA (3 eq) and Cbz-diamino propane HCI (1.0 eq) were 15 added and stirred for 3 hrs at room temperature. The reaction mixture was concentrated under a reduced pressure to give an oily residue, which was purified by preparative TLC to obtain the title compound. 'H-NMR (400MHz, CDCi 3 ) 8 ppm: 1.60(m, 2H), 2.01(s, 1H), 2.20 (s, 2H), 3.20(d, 2H), 3.24(m, 2H), 3.40 (d, 6H), 3.50(d, 2H), 4.06 (s, 2H), 4.44 20 (m, 1H), 5.08(s, 2H), 5.18(d, 1H), 6.91(brd, 1H), 7.16(br s, 5H); MS (mlz, ESI): 396(MH +) (C) Preparation of 8-acetyl-6-oxo-hexahydro-pyrazinol[1,2-alpvrimidine-1 carboxylic acid benzyl ester 0 Cbz O N 0 25 To a solution of the Cbz protected amide precursor obtained in the above step (B) in MC, PPTS (1 eq.) was added at room temperature and 89 WO 2004/108731 PCT/US2003/017188 heated to 70 0 C for 5 hrs. The resulting reaction mixture was concentrated to give a residue, which was characterized as follows. 'H-NMR (400 MHz, CDCI 3 ) 8 ppm: 1.90(m, 2H), 2.10(s, 1H), 2.30 (s, 2H), 2.61(m, 1H), 2.82(m, 1H), 3.15(m, 1H) 3.50(m, 1H), 3.9(m, 1H), 4.0 5 (m, 1H), 4.2(m, 1H), 4.3(s, 1H), 4.47 (m, 1H), 5.08-5.18(m, 2H), 5.28(br s, 1H), 7.16(br s, 5H); MS (m/z, ESI): 332(MH ) EXAMPLE 8 7-BENZOYLAMINO-4-BENZYLCARBAMOYL-6-OXO-HEXAHYDRO 10 PYRROLO[1,2-AiPYRIMIDINE-1 -CARBOXYLIC ACID METHYL ESTER (A) Preparation of [1-(1-benzylcarbamoyl-3-methoxycarbonylamino propylcarbamoyl)-3,3-dimethoxy-propyll-carbamic acid benzyl ester 0 HN AOCH 3 /O 00 N CONHBn NHCdz To a solution of the Cbz protected amino acid acetal (100mg, 15 1.3eq) obtained in the Preparative Example 3(3) in MC, PyBOP (1 eq to acid), DIPEA (6 eq to acid) and HOBt (1.3 eq) were added and stirred for 30 min. To the reaction mixture, amino benzyl amide HCI salt (71 mg, 0.27 mmol) was added and stirred for 7 hrs. The resulting reaction mixture was washed with sat. NaHCO 3 , water, and sat. NaCI. The organic layer was dried over MgSO 4 20 and concentrated to give an oily residue, which was purified by column chromatography (silica gel, ethyl acetate) to obtain the title compound (50mg, yield: 35%). 1 H-NMR (300MHz, CDCI 3 ) 8 ppm: 2.1(t, 2H), 3.05(m, 1H), 3.50(ss, 6H), 3.45(m, 1H), 3.75(s, 3H), 4.25(q, 1H), 4.41(m, 2H), 4.55(m, 1H), 25 5.0(q, 2H), 5.3(m, 1H), 5.95(m, 1H), 7.2 - 7.4(m, 10OH) 90 WO 2004/108731 PCT/US2003/017188 (B) Preparation of 4-benzylcarbamoyl-7-benzyloxycarbonylamino-6-oxo hexahydro-pyrrolol[1,2-alpyrimidine-l-carboxylic acid methyl ester O0 OCH 3 CbzHN N O O NHBn The acetal amide cyclization precursor (5mg, 0.009 mmol) 5 obtained in the above step (A) was dissolved in formic acid (1 mL) and stirred overnight. The resulting reaction mixture was concentrated to dryness, which is used in the next step without further purification. 1 H-NMR (300MHz, CDCI 3 ) 8 ppm: 2.25(m, 2H), 2.61(t, 2H), 3.24(m, 1H), 3.50(s, 3H), 3.55(m, 1H), 3.95(m, 1H), 4.45(m, 2H), 4.65(d, 1H), 10 4.8(m, 2H), 5.3(m, 1H), 5.7(d, 1H), 7.15-7.4(m, 10H), 7.85(m, 1H) (C) Preparation of 7-benzoylamino-4-benzylcarbamoyl-6-oxo-hexahydro pyrrolo[1,2-alpyrimidine-l-carboxylic acid methyl ester O OCH 3 BzHNj N+ O o NHBn In a reaction vessel equipped with a hydrogen gas balloon, a 15 solution of the Cbz bicyclic ring compound obtained in the above step (B) in MeOH and Pd/C (1 mg) were placed at room temperature and stirred for 2 hrs. After the reaction was complete, the reaction mixture was filtered by celite filter to remove Pd/C and the solvent was evaporated under reduced pressure. The resulting oily residue was dissolved in MC, to which a solution of benzoic 20 acid (1.1 eq) in MC and PyBOP (1.1 eq), HOBt (1.1 eq) and DIPEA (3 eq) were added and stirred for 30 min. To the resulting solution of the activated acid, an amine solution was added and kept stirring for 3 hrs. The resulting reaction mixture was concentrated under reduced pressure to give oily residue, which was purified by preparative TLC to obtain the title compound. 91 WO 2004/108731 PCT/US2003/017188 1 H-NMR (300MHz, CDCI 3 ) 8 ppm: 2.25(m, 2H), 2.65(m, 2H), 3.27(m, 1H), 3.70(s, 3H), 3.6(m, 1H), 4.10(m, 1H), 4.54(m, 2H), 4.8(t, 1H), 5.45(m, 1H), 7.15-7.42(m, 10OH), 7.9(d, 1H), 8.31(t, 1H) EXAMPLE 9 5 7-BENZOYLAMINO-4-(1 -CARBOXY-ETHYLCARBAMOYL)-6-OXO-HEXAHYDRO PYRROLO[1,2-A]PYRIMIDINE-1I-CARBOXYLIC ACID METHYL ESTER A synthetic scheme showing the methodology of Example 9 is presented in Figure 3. 2-Chlorotrityl chloride resin (200 mg, 1 mmol/g) and a solution of 10 Fmoc-Alanine (1.5 equiv. commercially available) and DIEA (2 equiv.) in DCE (2 mL) were placed in vial with screw cap. The reaction mixture was shaken at room temperature for 12 hours. The resin was collected by filtration and washed with DMF, MeOH, and then DCM, to provide a first component piece. To the resin swollen by DMF before reaction was added 25% 15 piperidine in DMF. Thereafter, the reaction mixture was shaken for 30 min at room temperature. The deprotection step was repeated and then the product mixture was washed with DMF, MeOH, and then DCM. A solution of 2-(9H fluoren-9-ylmethoxycarbonylamino)-4-methoxycarbonylamino-butyric acid (1.5 equiv. 2 nd component piece), DIC (1.5 equiv.), HOBT (1.5 equiv.) in NMP was 20 added to the resin. After the reaction mixture was shaken for 12 hours at room temperature, the resin was washed with DMF, MeOH, and then DCM. To the resin swollen by DMF before reaction was added 25% piperidine in DMF. Thereafter, the reaction mixture was shaken for 30 min at room temperature. The deprotection step was repeated and the product 25 mixture was washed with DMF, MeOH, and then DCM. A solution of 2-(9H fluoren-9-ylmethoxycarbonylamino)-5,5-dimethoxy-pentanoic acid (1.5 equiv.), DIC (1.5 equiv.), HOBT (1.5 equiv.) in NMP was added to the resin. After the reaction mixture was shaken for 12 hours at room temperature, the resin was washed with DMF, MeOH, and then DCM. 30 To the resin swollen by DMF before reaction was added 25% piperidine in DMF. Thereafter, the reaction mixture was shaken for 30 min at room temperature. The deprotection step was repeated and then the product mixture was washed with DMF, MeOH, and then DCM. A solution of commercially available benzoic acid (1.5 equiv.), DIC (1.5 equiv.), HOBT (1.5 35 equiv.) in NMP was added to the resin. After the reaction mixture was shaken 92 WO 2004/108731 PCT/US2003/017188 for 12 hours at room temperature, the resin was washed with DMF, MeOH, and then DCM. The resin was treated with formic acid (1.2 mL each well) for 18 hours at room temperature. Thereafter, the resin was removed by filtration 5 and the filtrate was condensed under reduced pressure to give the product as oil. 'HNMR (300 MHz, MeOH-d 4 ) 5 1.40 (d, 3H), 1.90 (m, 1H), 2.20 (m, 1H), 2.30 ~ 2.50 (m, 2H), 3.15 (m, 1H), 3.20 (m, 1H), 3.45 (s, 3H), 3.40 ~ 3.60 (m, 1 H), 4.20 ~ 4.40 (m, 2H), 4.70 (t, 1 H), 5.40 (t, 1 H), 7.25 ~ 7.45 (m, 3H), 7.75 (d, 2H). 10 MS(m/z, ESI): 433 (MH*), 455 (MNa') EXAMPLE 10 7-BENZOYLAMINO-4-(2-CARBOXY-PROPYLCARBAMOYL)-6-OXO-HEXAHYDRO PYRROLO[1,2-A]PYRIMIDINE-1I-CARBOXYLIC ACID METHYL ESTER A synthetic scheme showing the methodology of Example 10 is 15 presented in Figure 4. 2-Chlorotrityl chloride resin (200 mg, 1 mmollg) and a solution of Fmoc-beta-alanine (1.5 equiv.) and DIEA (2 equiv.) in DCE (2 mL) were placed in a vial with screw cap. The reaction mixture was shaken at room temperature for 12 hours. The resin was collected by filtration and washed 20 with DMF, MeOH, and then DCM, to provide a first component piece. To the resin swollen by DMF before reaction was added 25% piperidine in DMF. Thereafter, the reaction mixture was shaken for 30 min at room temperature. The deprotection step was repeated and the product mixture was washed with DMF, MeOH, and then DCM. A solution of 2-(9H 25 fluoren-9-ylmethoxycarbonylamino)-4-methoxycarbonylamino-butyric acid (1.5 equiv. 2 nd component piece), DIC (1.5 equiv.), and HOBT (1.5 equiv.) in NMP was added to the resin. After the reaction mixture was shaken for 12 hours at room temperature, the resin was washed with DMF, MeOH, and then DCM. To the resin swollen by DMF before reaction was added 25% 30 piperidine in DMF. Thereafter, the reaction mixture was shaken for 30 min at room temperature. The deprotection step was repeated and then the product mixture was washed with DMF, MeOH, and then DCM. A solution of 2-(9H fluoren-9-ylmethoxycarbonylamino)-5,5-dimethoxy-pentanoic acid (1.5 equiv.), DIC (1.5 equiv.), and HOBT (1.5 equiv.) in NMP was added to the resin. After 93 WO 2004/108731 PCT/US2003/017188 the reaction mixture was shaken for 12 hours at room temperature, the resin was washed with DMF, MeOH, and then DCM. To the resin swollen by DMF before reaction was added 25% piperidine in DMF. Thereafter, the reaction mixture was shaken for 30 min at 5 room temperature. The deprotection step was repeated and then the product mixture was washed with DMF, MeOH, and then DCM. A solution of commercially available benzoic acid (1.5 equiv.), DIC (1.5 equiv.), and HOBT (1.5 equiv.) in NMP was added to the resin. After the reaction mixture was shaken for 12 hours at room temperature, the resin was washed with DMF, 10 MeOH, and then DCM. The resin was treated with formic acid (1.2 mL in each well) for 18 hours at room temperature. After the resin was removed by filtration, the filtrate was condensed under reduced pressure to give the product as oil. 1 HNMR (300 MHz, MeOH-d 4 ) 8 1.40 (d, 3H), 1.90 (m, 1H), 2.20 (m, 1H), 2.30 ~ 15 2.50 (m, 2H), 3.15 (m, 2H), 3.35 (s, 3H), 3.40 ~ 3.60 (m, 3H), 4.20 ~ 4.40 (m, 2H), 4.70 (t, 1H), 5.40 (t, 1H), 7.25 - 7.45 (m, 3H), 7.75 (d, 2H). MS(m/z, ESI): 447 (MH+), 469 (MNa +) Various references are set forth herein, which describe in detail 20 certain procedures, compounds and/or compositions, and are incorporated by reference in their entirety. It will be appreciated that, although specific embodiments of the invention have been described herein for the purposes of illustration, various modifications may be made without departing from the spirit and scope of the 25 invention. Accordingly, the invention is not limited except by the appended claims. 94
权利要求:
Claims (27) [1] 1. A compound having the structure: R1 W / z R 2 X- B N N Y L wherein A is -(CH)-, -N- or -CH 2 -N-, B is -(C=0)- or -(CH 2 ) m-, W is -(C=0)-, -Y(C=0)-, -NH(C=0)- or nothing, X is -NH-, -NH(C=O)- or nothing, Y is oxygen or sulfur, Z is oxygen or hydrogen, L is hydrogen, R 5 s, -C(=0)NHRa or its equivalents, n=0 or 1 and m=l or 2; R 1 , R 2 , R 3 , R 4 and R 5 are the same or different and independently selected from hydrogen, an amino acid side chain moiety or derivative thereof, the remainder of the molecule, a linker and a solid support, and stereoisomers thereof. [2] 2. The compound of claim 1, wherein R 1 , R 2 , R 3 , R 4 and Rs are independently selected from the group consisting of aminoC 2 - 5 alkyl, guanidinoC 2 -5alkyl, C 1 - 4 alkylguanidinoC 2 -5alkyl, diC 1 - 4 alkylguanidino-C 2 -5alkyl, amidinoC 2 -salkyl, Cl-4alkylamidinoC 2 -5alkyl, diC 1 - 4 alkylamidinoC 2 -5alkyl, Cj1 3 alkoxy, phenyl, substituted phenyl (where the substituents are independently selected from one or more of amino, amidino, guanidine, hydrazine, amidrazonyl, C1- 4 alkylamino, C 1 - 4 dialkylamino, halogen, perfluoro C1-4alkyl, C 1 4 alkyl, C 1 .- 3 alkoxy, nitro, carboxy, cyano, sulfuryl, or hydroxyl), benzyl, substituted benzyl (where the substituents on the benzyl are independently selected from one or more of amino, amidino, guanidino, hydrazino, amidrazonyl, C1-4alkylamino, C1- 4 dialkylamino, halogen, perfluoro C1-4alkyl, C1. 3 alkoxy, nitro, carboxy, cyano, sulfuryl, or hydroxyl), naphthyl, substituted naphthyl (where the substituents are independently selected from one or more of amino, amidino, guanidine, hydrazine, amidrazonyl, C1- 4 alkylamino, C 1 . 4 dialkylamino, halogen, perfluoro C 1 4 alkyl, C 1 - 4 alkyl, C 1 .- 3 alkoxy, nitro, 95 WO 2004/108731 PCT/US2003/017188 carboxy, cyano, sulfuryl, or hydroxyl), bis-phenyl methyl, substituted bis-phenyl methyl (where the substituents are independently selected from one or more of amino, amidino, guanidine, hydrazine, amidrazonyl, C 1 - 4 alkylamino, Cl- 4 dialkylamino, halogen, perfluoro C 1 - 4 alkyl, C 1 4 alkyl, CI- 3 alkoxy, nitro, carboxy, cyano, sulfuryl, or hydroxyl), pyridyl, substituted pyridyl (where the substituents are independently selected from one or more of amino amidino, guanidino, hydrazino, amidrazonyl, C 1 - 4 alkylamino, C 1 . 4 dialkylamino, halogen, perfluoro C 1 . 4 alkyl, C 14 alkyl, C 1 . 3 alkoxy, nitro, carboxy, cyano, sulfuryl, or hydroxyl), pyridylC1.4alkyl, substituted pyridylCI- 4 alkyl (where the pyridine substituents are independently selected from one or more of amino, amidino, guanidino, hydrazino, amidrazonyl, C 1 G- 4 alkylamino, C 1 . 4 dialkylamino, halogen, perfluoro C 1 - 4 alkyl, C 1 - 4 alkyl, C1- 3 alkoxy, nitro, carboxy, cyano, sulfuryl, or hydroxyl), pyrimidylC 1 - 4 alkyl, substituted pyrimidylC 1 - 4 alkyl (where the pyrimidine substituents are independently selected from one or more of amino, amidino, guanidine, hydrazine, amidrazonyl, C 1 - 4 alkylamino, C1-4dialkylamino, halogen, perfluoro C1-4alkyl, C 1 .- 4 alkyl, C 1 -3alkoxy, nitro, carboxy, cyano, sulfuryl, or hydroxyl), triazin-2-yl-C 1 - 4 alkyl, substituted triazin-2-yl-C.- 4 alkyl (where the triazine substituents are independently selected from one or more of amino, amidino, guanidine, hydrazine, amidrazonyl, C1- 4 alkylamino, C 1 - 4 dialkylamino, halogen, perfluoro C 1 - 4 alkyl, C1- 4 alkyl, Cs. 3 alkoxy, nitro, carboxy, cyano, sulfuryl, or hydroxyl), imidazoC1-4alkyl, substituted imidazol C. 4 alkyl (where the imidazole sustituents are independently selected from one or more of amino, amidino, guanidine, hydrazine, amidrazonyl, C1- 4 alkylamino, C 1 - 4 dialkylamino, halogen, perfluoro C 1 - 4 alkyl, C 1 -4alkyl, C1- 3 alkoxy, nitro, carboxy, cyano, sulfuryl, or hydroxyl), imidazolinylC 1 .- 4 alkyl, N-amidino piperazinyl-N-CO- 4 alkyl, hydroxyC 2 - 5 alkyl, C 15 -alkylaminoC 2 -5alkyl, C 1 s 5 dialkyl aminoC 2 - 5 alkyl, N-amidinopiperidinylC 1 - 4 alkyl and 4-aminocyclohexylCo- 2 alkyl. 96 WO 2004/108731 PCT/US2003/017188 [3] 3. The compound of claim 1 wherein X is absent, A is N, B is -(C=O)- and L is -C(O)NHR 3 , and the compound has the structure: R1 O W /z N R 2 -N N R Y O N R 3 O N R H [4] 4. The compound of claim 3 wherein W is absent and Z is oxygen. [5] 5. The compound of claim 1 wherein X is absent, A is N, B is -(CH 2 ) m- and L is -C(O)NHR 3 , and the compound has the structure: RI w I/ z M N N n n Y H [6] 6. The compound of claim 5 wherein W is absent and Z is oxygen. 97 WO 2004/108731 PCT/US2003/017188 [7] 7. The compound of claim 1 wherein X is -NH-, A is -(CH)-, B is -(CH 2 )m- and L is -C(O)NHR 3 , and the compound has the structure: R 1 W / z Rm N/ N H N R 2q1 n Y 1 NR3 H [8] 8. The compound of claim 7 wherein Z is oxygen and W is absent. [9] 9. The compound of claim 1 wherein A is -CH 2 -N-, B is - (CH 2 ) m- and L is -C(O)NHR 3 , and the compound has the structure: R1 W X ( )m R2N ( )n R4 Y N /R3 H [10] 10. The compound of claim 9 wherein Y is oxygen. [11] 11. The compound of claim 10 wherein W is absent and Z is oxygen. [12] 12. The compound of claim 9 wherein W is absent and Z is oxygen. 98 WO 2004/108731 PCT/US2003/017188 [13] 13. The compound of claim 1 wherein A is -(CH)-, B is -(CH 2 ) m- with m = 1, W is -(C=0)- or nothing, X is-NH(C=0)-, Y is oxygen, Z is hydrogen so that C=Z represents CH 2 , L is -C(=0)NHR 3 , n=0, R 4 is hydrogen, and R 1 , R 2 , and R 3 are the same or different and are independently selected from an amino acid side chain moiety or derivative thereof, the remainder of the molecule, a linker and a solid support, and stereoisomers thereof. [14] 14. The compound of claim 13 wherein R 1 , R 2 , and R 3 are independently selected from organic groups having molecular weights of between 15 and 1,000 g/mol; and at least one of R 1 , R 2 , and R 3 represents an amino acid side chain or derivative thereof. [15] 15. The compound of any one of claims 1-14 wherein R 3 is joined to a solid support or solid support derivatives. [16] 16. A library of compounds, comprising at least one compound of any one of claims 1-14. [17] 17. A pharmaceutical composition comprising a compound of claims 1-14 and pharmaceutically acceptable carrier. [18] 18. A method of identifying a biologically active compound, comprising contacting the library of claim 17 with a target to detect or screen the biologically active compound. [19] 19. A method for inhibiting a kinase in a warm-blooded animal, comprising administering to the animal an amount of a compound of any of claims 1-14 effective to inhibit the kinase. [20] 20. A method for CAAX inhibition in a warm-blooded animal, comprising administering to the animal an amount of a compound of any of claims 1-14 effective to inhibit CAAX. 99 WO 2004/108731 PCT/US2003/017188 [21] 21. A method for inhibiting a protease in a warm-blooded animal comprising administering to the animal an amount of a compound of any of claims 1-14 effective to inhibit the protease. [22] 22. A method for inhibiting the binding of an antigenic peptide to either a class one or class two MHC molecule, comprising contacting an amount of a compound of any of claims 1-14 with a composition comprising an antigenic peptide and either a class one or class two MHC molecule, where the amount is effective to inhibit the binding. [23] 23. A method for inhibiting the binding of a phosphotyrosine residue present in a first peptide, to an SH2 domain in a second peptide, comprising contacting an amount of a compound of any of claims 1-14 with a composition comprising the first peptide and the second peptide, where the amount is effective to inhibit the binding. [24] 24. A method for inhibiting the binding of a proline-rich region of a first peptide to a SH3 domain in a second peptide, comprising contacting an amount of a compound of any of claims 1-14 with a composition comprising the first peptide and the second peptide, where the amount is effective to inhibit the binding. [25] 25. A method for inhibiting an oxidoreductase in a warm blooded animal, comprising administering to the animal an amount of a compound of any of claims 1-14, where the amount is effective to inhibit the oxidoreductase. [26] 26. A method for inhibiting a phosphatase in a warm-blooded animal, comprising administering to the animal an amount of a compound of any of claims 1-14, where the amount is effective to inhibit the phosphatase. [27] 27. A method for inhibiting the binding of a first peptide to a 14-3-3 domain in a second peptide, comprising contacting a compound of any of claims 1-14 with an amount of a composition comprising the first peptide that has a binding affinity to a 14-3-3 domain of a second peptide, and a second peptide having a 14-3-3 domain, where the amount is effective to inhibit the binding. 100
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公开号 | 公开日 AT428713T|2009-05-15| AU2003249672B2|2008-03-06| CN1802378A|2006-07-12| KR20060056896A|2006-05-25| JP4546923B2|2010-09-22| ES2323172T3|2009-07-08| NZ543790A|2009-09-25| CA2527375A1|2004-12-16| BRPI0318325B8|2021-05-25| CA2527375C|2010-08-10| DE60327263D1|2009-05-28| KR101018453B1|2011-03-02| EP1646633A1|2006-04-19| BRPI0318325B1|2019-06-04| EP1646633B1|2009-04-15| WO2004108731A1|2004-12-16| BR0318325A|2006-07-18| JP2006526568A|2006-11-24|
引用文献:
公开号 | 申请日 | 公开日 | 申请人 | 专利标题 DE2948535A1|1979-12-03|1981-06-25|Basf Ag, 6700 Ludwigshafen|DICHLORACETAMIDES, HERBICIDE AGENTS THAT CONTAIN ACETANILIDES AS HERBICIDAL ACTIVE SUBSTANCES AND THESE DICHLORACETAMIDES AS AN ANTAGONISTIC AGENTS, AND THEIR USE FOR CONTROLLING UNWANTED PLANT GROWTH| DE3120859A1|1981-05-26|1982-12-23|Basf Ag, 6700 Ludwigshafen|DIHALOGEN ACETAMIDES, METHOD FOR THE PRODUCTION THEREOF AND HERBICIDAL AGENTS THAT CONTAIN ACETANILIDES AS AN HERBICIDE ACTIVE SUBSTANCES AND THESE DIHALOGEN ACETAMIDES AS AN ANTAGONISTIC AGENTS| AU2002303779A1|2001-05-16|2002-11-25|Molecumetics, Ltd.|Reverse-turn mimetics and methods relating thereto|WO2005077040A2|2004-02-11|2005-08-25|Rensselaer Polytechnic Institute|Compositions and methods for treating amyotrophic lateral sclerosis | US20120296067A1|2010-02-03|2012-11-22|Prism BioLab Co., Ltd.|Compound capable of binding to naturally occurring denatured protein, and method for screening for the compound| EP3560932A4|2016-12-21|2020-10-28|Japan Tobacco, Inc.|Method for producing 7h-pyrrolo[2,3-d]pyrimidine derivative and synthetic intermediate of same|
法律状态:
2008-07-03| FGA| Letters patent sealed or granted (standard patent)|
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